Team:Paris Bettencourt/YonatanTest
From 2013.igem.org
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- | <p style="margin-top:-60px;font-size:14px"> | + | <p style="margin-top:-60px;font-size:14px">CRISPR anti-Kan plasmids target kanamycin resistant E. coli. WT (blue bars) and a kanamycin resistant strain (KanR, red bars) were co-transformed with a plasmid carrying the Cas9 construct, and a second plasmid carrying the anti-Kanamycin gRNA. WT E. coli was transformed with one and both plasmids. KanR E. coli couldn’t be tranformed with both plasmids because of Cas9-induced cleavage of the chromosome specifically at the KanR cassette, with about 99% efficiency. |
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Revision as of 12:08, 25 October 2013
![](https://static.igem.org/mediawiki/2013/d/d9/PB_achievementsbanenr.png)
Detect
Background
CRISPR/Cas systems generate site-specific double strand breaks and have recently been used for genome editing.
Aims
Building a genotype sensor based on CRISPR/Cas that reports existance of an antibiotic resistance gene.
Results
- Successfully cloned gRNA anti-KAN, crRNA anti-KAN, tracrRNA-Cas9 and pRecA-LacZ into Biobrick backbones and therefore generated four new BioBricks.
- Testing the new assembly standard for our cloning.
![](https://static.igem.org/mediawiki/2013/5/55/PB_Bb_target.png)
![](https://static.igem.org/mediawiki/2013/9/99/PB_fig_target.png)
CRISPR anti-Kan plasmids target kanamycin resistant E. coli. WT (blue bars) and a kanamycin resistant strain (KanR, red bars) were co-transformed with a plasmid carrying the Cas9 construct, and a second plasmid carrying the anti-Kanamycin gRNA. WT E. coli was transformed with one and both plasmids. KanR E. coli couldn’t be tranformed with both plasmids because of Cas9-induced cleavage of the chromosome specifically at the KanR cassette, with about 99% efficiency.