Team:NJU China/Parts

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<h1>Acknowledgement</h1>
 
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     <li><a href="https://2013.igem.org/Team:NJU_China/Project">Project</a>  
     <li><a href="https://2013.igem.org/Team:NJU_China/Project">Project</a>  
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  <li><a href="http://https://2013.igem.org/Team:NJU_China/Project#Overview">Overview</a></li>  
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  <li><a href="https://2013.igem.org/Team:NJU_China/Project">Overview</a></li>  
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           <li><a https://2013.igem.org/Team:NJU_China/Project#Chassis">Chassis</a></li>  
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           <li><a href="https://2013.igem.org/Team:NJU_China/Project">Chassis</a></li>  
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           <li><a https://2013.igem.org/Team:NJU_China/Project#Targeting Module">Targeting Module</a></li>  
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           <li><a href="https://2013.igem.org/Team:NJU_China/Project">Targeting Module</a></li>  
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           <li><a https://2013.igem.org/Team:NJU_China/Project#Killing Module">Killing Module</a></li>  
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           <li><a href="https://2013.igem.org/Team:NJU_China/Project">Killing Module</a></li>  
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           <li><a https://2013.igem.org/Team:NJU_China/Project#Achievement">Achievement</a></li>
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           <li><a href="https://2013.igem.org/Team:NJU_China/Project">Achievement</a></li>
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     <li><a href="https://2013.igem.org/Team:NJU_China/Human Practice">Human Practice</a>
     <li><a href="https://2013.igem.org/Team:NJU_China/Human Practice">Human Practice</a>
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  <li><a href="http://https://2013.igem.org/Team:NJU_China/Project#Free debate">Free debate</a></li>  
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  <li><a href="https://2013.igem.org/Team:NJU_China/Human Practice">Free debate</a></li>  
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           <li><a https://2013.igem.org/Team:NJU_China/Project#Reach the unreached">Reach the unreached</a></li>  
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           <li><a href="https://2013.igem.org/Team:NJU_China/Human Practice">Reach the unreached</a></li>  
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           <li><a https://2013.igem.org/Team:NJU_China/Project#Workshop">Workshop</a></li>  
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           <li><a href="https://2013.igem.org/Team:NJU_China/Human Practice">Workshop</a></li>  
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           <li><a https://2013.igem.org/Team:NJU_China/Project#Collaboration">Collaboration</a></li>  
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           <li><a href="https://2013.igem.org/Team:NJU_China/Human Practice">Collaboration</a></li>  
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     <li><a href="https://2013.igem.org/Team:NJU_China/Wet lab">Wet lab</a>
     <li><a href="https://2013.igem.org/Team:NJU_China/Wet lab">Wet lab</a>
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  <li><a href="http://https://2013.igem.org/Team:NJU_China/Project#Protocol">Protocol</a></li>  
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  <li><a href="https://2013.igem.org/Team:NJU_China/Protocol">Protocol</a></li>  
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           <li><a https://2013.igem.org/Team:NJU_China/Project#Reach the unreached">Lab notes</a></li>  
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           <li><a href="https://2013.igem.org/Team:NJU_China/Notebook">Lab notes</a></li>  
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           <li><a https://2013.igem.org/Team:NJU_China/Project#Workshop">Data Page</a></li>  
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           <li><a href="https://2013.igem.org/Team:NJU_China/Data Page">Data Page</a></li>  
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           <li><a https://2013.igem.org/Team:NJU_China/Project#Collaboration">Parts</a></li>  
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           <li><a href="https://2013.igem.org/Team:NJU_China/Parts">Parts</a></li>  
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     <li><a href="https://2013.igem.org/Team:NJU_China/Safety">Safety</a>
     <li><a href="https://2013.igem.org/Team:NJU_China/Safety">Safety</a>
         <ul>  
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  <li><a href="http://https://2013.igem.org/Team:NJU_China/Project#Protocol">Safety form</a></li>  
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  <li><a href="https://2013.igem.org/Team:NJU_China/Safety">Safety form</a></li>  
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           <li><a https://2013.igem.org/Team:NJU_China/Project#Reach the unreached">Virus-related safety</a></li>  
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           <li><a href="https://2013.igem.org/Team:NJU_China/Virus-related_safety">Virus-related safety</a></li>  
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     <li><a href="https://2013.igem.org/Team:NJU_China/Extras">Extras</a>
     <li><a href="https://2013.igem.org/Team:NJU_China/Extras">Extras</a>
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  <li><a href="http://https://2013.igem.org/Team:NJU_China/Project#Protocol">Judging criteria</a></li>  
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  <li><a href="https://igem.org/2013_Judging_Form?id=1180">Judging criteria</a></li>  
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           <li><a https://2013.igem.org/Team:NJU_China/Project#Reach the unreached">Attribution</a></li>  
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           <li><a href="https://2013.igem.org/Team:NJU_China/Attributions">Attribution</a></li>  
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           <li><a https://2013.igem.org/Team:NJU_China/Project#Reach the unreached">Acknowledgement</a></li>
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           <li><a href="https://2013.igem.org/Team:NJU_China/Acknowledgement">Acknowledgement</a></li>
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</br>    <li><a href="https://2013.igem.org/Team:NJU_China/Safety">Safety form</a>
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</br>    <li><a href="https://2013.igem.org/Team:NJU_China/Notebook">Notebook</a>
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<li><a href="https://2013.igem.org/Team:NJU_China/Protocol">Protocol</a>
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<li><a href="https://2013.igem.org/Team:NJU_China/Data_Page">Data Page</a>
    
    
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<h1>parts</h1>
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During the past seven months, we received help from various sources. Especially our instructor Prof. Chen Xi gives us a lot of help <br>when we carry out our project. <br>
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This year our team iGEM-China constructed altogether 3 parts.<br><br>
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We thank all the person and institution where we received help: <br>
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Prof. Zhang Chenyu<br>
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Prof. Chen Xi<br>
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Prof. Kong Lingdong<br>
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Wang Xueliang<br>
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Liu Yanqing<br>
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Liang Hongwei<br>
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Wu Xin<br>
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Qiu Ruosang<br>
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Zhang Sitao<br>
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Chen Shuobing<br>
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Sponsor:<br>
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<img src="https://static.igem.org/mediawiki/2012/0/0a/M3-Acknowledgement111.png"/><br><br><br>
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<img src="https://static.igem.org/mediawiki/2012/5/50/M3-Acknowledgement11.png"/><br><br><br>
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<img src="https://static.igem.org/mediawiki/2012/2/28/M3-Acknowledgement1.png"/><br><br><br>
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For the killing module, we constructed plasmid encoding anti-HBV siRNA 467(<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1180000">BBa_K1180000</a>) and cloned this into standardized backbone pSB1C3. The function of this part was extensively validated by us and it worked as we expected. The expression level of this plasmid in the chassis HEK 293T cells is high and it can silence the viral gene HBsAG significantly.<br><br>
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For the targeting module, we constructed <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1180002">BBa_K1180002</a> (brain targeting) and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1180003">BBa_K1180003</a> (liver targeting). The function of was extensively confirmed by both in vitro and in vivo test. After its expression, the RVG peptide can lead the exosome to the brain.<br>
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:NJU_China|Home]]
 
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!align="center"|[[Team:NJU_China/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=NJU_China Official Team Profile]
 
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!align="center"|[[Team:NJU_China/Project|Project]]
 
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!align="center"|[[Team:NJU_China/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:NJU_China/Modeling|Modeling]]
 
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!align="center"|[[Team:NJU_China/Notebook|Notebook]]
 
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!align="center"|[[Team:NJU_China/Safety|Safety]]
 
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!align="center"|[[Team:NJU_China/Attributions|Attributions]]
 
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An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will place them in the [http://partsregistry.org Registry of Standard Biological Parts]. The iGEM software provides an easy way to present the parts your team has created . The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
 
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Remember that the goal of proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for  users who wish to know more.
 
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<groupparts>iGEM013 NJU_China</groupparts>
<groupparts>iGEM013 NJU_China</groupparts>

Latest revision as of 09:11, 28 October 2013

<!DOCTYPE html> NJU_China






parts

This year our team iGEM-China constructed altogether 3 parts.

For the killing module, we constructed plasmid encoding anti-HBV siRNA 467(BBa_K1180000) and cloned this into standardized backbone pSB1C3. The function of this part was extensively validated by us and it worked as we expected. The expression level of this plasmid in the chassis HEK 293T cells is high and it can silence the viral gene HBsAG significantly.

For the targeting module, we constructed BBa_K1180002 (brain targeting) and BBa_K1180003 (liver targeting). The function of was extensively confirmed by both in vitro and in vivo test. After its expression, the RVG peptide can lead the exosome to the brain.

<groupparts>iGEM013 NJU_China</groupparts>

Retrieved from "http://2013.igem.org/Team:NJU_China/Parts"