Team:NJU China/Parts

From 2013.igem.org

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This is a template page. READ THESE INSTRUCTIONS.
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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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<div style="float:left; position:static; margin-top:-15px;margin-left:-60px; z-index:1; display:block; ">
 +
</br></br>
 +
<a href="https://2013.igem.org"><img src="https://static.igem.org/mediawiki/2013/8/80/NJU-Miniminiminilogocolored.png" ></a>
</div>
</div>
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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-
You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified.   PLEASE keep all of your pages within your teams namespace.
+
<div class="container">
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</div>
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<!--Start NavBar-->
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</div>
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<ul id="nav">
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</html>
+
    <li><a href="https://2013.igem.org/Team:NJU_China">Home</a></li>
 +
 +
    <li><a href="https://2013.igem.org/Team:NJU_China/Project">Project</a>
 +
        <ul>
 +
  <li><a href="https://2013.igem.org/Team:NJU_China/Project">Overview</a></li>  
 +
          <li><a href="https://2013.igem.org/Team:NJU_China/Project">Chassis</a></li>
 +
          <li><a href="https://2013.igem.org/Team:NJU_China/Project">Targeting Module</a></li>
 +
          <li><a href="https://2013.igem.org/Team:NJU_China/Project">Killing Module</a></li>
 +
          <li><a href="https://2013.igem.org/Team:NJU_China/Project">Achievement</a></li>
 +
        </ul>
 +
    </li>
 +
    <li><a href="https://2013.igem.org/Team:NJU_China/Modeling">Modeling</a>
 +
    </li>
 +
 +
    <li><a href="https://2013.igem.org/Team:NJU_China/Human Practice">Human Practice</a>
 +
        <ul>
 +
  <li><a href="https://2013.igem.org/Team:NJU_China/Human Practice">Free debate</a></li>
 +
          <li><a href="https://2013.igem.org/Team:NJU_China/Human Practice">Reach the unreached</a></li>
 +
          <li><a href="https://2013.igem.org/Team:NJU_China/Human Practice">Workshop</a></li>
 +
          <li><a href="https://2013.igem.org/Team:NJU_China/Human Practice">Collaboration</a></li>  
 +
        </ul>
 +
    </li>
-
<!-- *** End of the alert box *** -->
+
    <li><a href="https://2013.igem.org/Team:NJU_China/Wet lab">Wet lab</a>
 +
        <ul>
 +
  <li><a href="https://2013.igem.org/Team:NJU_China/Protocol">Protocol</a></li>
 +
          <li><a href="https://2013.igem.org/Team:NJU_China/Notebook">Lab notes</a></li>
 +
          <li><a href="https://2013.igem.org/Team:NJU_China/Data Page">Data Page</a></li>
 +
          <li><a href="https://2013.igem.org/Team:NJU_China/Parts">Parts</a></li>
 +
        </ul>
 +
    </li>
 +
   
 +
    <li><a href="https://2013.igem.org/Team:NJU_China/Safety">Safety</a>
 +
        <ul>
 +
  <li><a href="https://2013.igem.org/Team:NJU_China/Safety">Safety form</a></li>
 +
          <li><a href="https://2013.igem.org/Team:NJU_China/Virus-related_safety">Virus-related safety</a></li>
 +
        </ul>
 +
    </li>  
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
    <li><a href="https://2013.igem.org/Team:NJU_China/Team">Team</a></li>
-
!align="center"|[[Team:NJU_China|Home]]
+
-
!align="center"|[[Team:NJU_China/Team|Team]]
+
-
!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=NJU_China Official Team Profile]
+
-
!align="center"|[[Team:NJU_China/Project|Project]]
+
-
!align="center"|[[Team:NJU_China/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:NJU_China/Modeling|Modeling]]
+
-
!align="center"|[[Team:NJU_China/Notebook|Notebook]]
+
-
!align="center"|[[Team:NJU_China/Safety|Safety]]
+
-
!align="center"|[[Team:NJU_China/Attributions|Attributions]]
+
-
|}
+
 +
    <li><a href="https://2013.igem.org/Team:NJU_China/Extras">Extras</a>
 +
            <ul>
 +
  <li><a href="https://igem.org/2013_Judging_Form?id=1180">Judging criteria</a></li>
 +
          <li><a href="https://2013.igem.org/Team:NJU_China/Attributions">Attribution</a></li>
 +
          <li><a href="https://2013.igem.org/Team:NJU_China/Acknowledgement">Acknowledgement</a></li>
 +
        </ul>
 +
    </li>
 +
   
 +
</br>    <li><a href="https://2013.igem.org/Team:NJU_China/Notebook">Notebook</a>
 +
<li><a href="https://2013.igem.org/Team:NJU_China/Protocol">Protocol</a>
 +
<li><a href="https://2013.igem.org/Team:NJU_China/Data_Page">Data Page</a>
 +
   
 +
</ul>
 +
<!--End NavBar-->
 +
</br></br></br>
-
An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will place them in the [http://partsregistry.org Registry of Standard Biological Parts]. The iGEM software provides an easy way to present the parts your team has created . The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
+
                        <header>
 +
<h1>parts</h1>
 +
</header>
 +
-
Remember that the goal of proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for  users who wish to know more.
+
<section>
 +
<p>
 +
This year our team iGEM-China constructed altogether 3 parts.<br><br>
 +
 
 +
 
 +
For the killing module, we constructed plasmid encoding anti-HBV siRNA 467(<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1180000">BBa_K1180000</a>) and cloned this into standardized backbone pSB1C3. The function of this part was extensively validated by us and it worked as we expected. The expression level of this plasmid in the chassis HEK 293T cells is high and it can silence the viral gene HBsAG significantly.<br><br>
 +
 
 +
For the targeting module, we constructed <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1180002">BBa_K1180002</a> (brain targeting) and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1180003">BBa_K1180003</a> (liver targeting). The function of was extensively confirmed by both in vitro and in vivo test. After its expression, the RVG peptide can lead the exosome to the brain.<br>
 +
 
 +
</p>
 +
 
 +
</section>
 +
</div><!-- /container -->
 +
 
 +
</body>
 +
 
 +
</html>
<groupparts>iGEM013 NJU_China</groupparts>
<groupparts>iGEM013 NJU_China</groupparts>

Latest revision as of 09:11, 28 October 2013

<!DOCTYPE html> NJU_China






parts

This year our team iGEM-China constructed altogether 3 parts.

For the killing module, we constructed plasmid encoding anti-HBV siRNA 467(BBa_K1180000) and cloned this into standardized backbone pSB1C3. The function of this part was extensively validated by us and it worked as we expected. The expression level of this plasmid in the chassis HEK 293T cells is high and it can silence the viral gene HBsAG significantly.

For the targeting module, we constructed BBa_K1180002 (brain targeting) and BBa_K1180003 (liver targeting). The function of was extensively confirmed by both in vitro and in vivo test. After its expression, the RVG peptide can lead the exosome to the brain.

<groupparts>iGEM013 NJU_China</groupparts>

Retrieved from "http://2013.igem.org/Team:NJU_China/Parts"