Team:Hong Kong HKUST/results

From 2013.igem.org

(Difference between revisions)

Latest revision as of 12:53, 28 October 2013

Result

Experiment 1: Glyoxylate Shunt

Introduce glyoxylate enzymes responsible for the shunt

Experiment 2: Protein Trafficking

Target ACE proteins into mitochondria

Experiment 3: Fatty Acid Sensing Mechanism

Introduce inducible system that allows tunable fatty acid uptake by sensing fatty acid concentration

Experiment 4: Cell Viability & Fatty Acid Quantification

Measuring cell viability at different fatty acid concentration & measure fatty acid uptake rate

Our project was first proposed in mid-March and after a series of improvement and elaboration, we started our wet lab work from mid-June to late-September. After 3 months of memorable diligent work, we are proud to claim that we have achieved the results below:

Successfully constructed two parts carrying mitochondrial leader sequence (MLS) compatible with RFC 10 and RFC 25 assembly standards respectively, (BBa_K1119000, BBa_K1119001).
Successfully constructed parts containing the aceA and aceB elements of the glyoxylate shunt (BBa_K1119002, BBa_K1119003).
Successfully constructed a part containing the constitutive CMV promoter (BBa_K1119006).
Successfully characterized the constitutive CMV promoter in HEK293FT cells (BBa_K1119008).
Successfully constructed a part containing the constitutive regulatory promoter of Human Elongation Factor 1-alpha (BBa_K1119010).
Successfully characterized the constitutive EF-1alpha promoter in HEK293FT cells (BBa_K1119011).
Successfully characterized the mitochondrial leader sequence (MLS) in HEK293FT cells (BBa_K1119009).
Successfully tested HepG2 cell viability in different sodium palmitate concentrations.

@@ Line 120: / Line 120: @@
 }
 #slide{width:96%;height:auto;background-color:#F4DFC0;margin-top:80px;border:2px solid;
+border-radius:15px;padding-right:10px;margin-left:15px;font-family:"Trebuchet MS",Helvetica,sans-serif;float:left;}
+#slide1{width:96%;height:auto;background-color:#F4DFC0;margin-top:20px;border:2px solid;
 border-radius:15px;padding-right:10px;margin-left:15px;font-family:"Trebuchet MS",Helvetica,sans-serif;float:left;}
 .center
@@ Line 129: / Line 131: @@
 #interview,#presentation,#article, #interview1, #presentation1,#article1
 {margin-top:20px;width:23%;margin-right:10px;float:left;height:230px; border:2px solid;
-border-radius:15px;font-family:"Trebuchet MS", Helvetica, sans-serif;margin-bottom:25px;}
+border-radius:15px;font-family:"Trebuchet MS", Helvetica, sans-serif;}
 #interview
 {margin-left:0px;background:#e32e51;}
@@ Line 143: / Line 145: @@
 #art-desc-text,#pre-desc-text,#int-desc-text{float:right;}
 h3,h1,#yo{margin-left:10px;font-family:"Trebuchet MS", Helvetica, sans-serif;}
-h3.title{font-size:35px;}
+h3.title{font-size:38px;}
+#iGEM_Logo{
+width:100px;
+height:80px;
+position:absolute;
+right:10px;
+top:60px;
+z-index:+15;
+}
+#hkust_Logo{
+width:60px;
+height:80px;
+position:absolute;
+right:110px;
+top:60px;
+z-index:+15;
+}
 </style>
 </head>
 <body>
+           <a href="https://2013.igem.org/Main_Page"><img id="iGEM_Logo" src="https://static.igem.org/mediawiki/2013/4/46/Igem_qgem_logo.png"></a>
+<a href="http://www.ust.hk/eng/index.htm"><img id="hkust_Logo" src="https://static.igem.org/mediawiki/2013/5/55/Hkust_logo.gif"></a>
 <a href=https://2013.igem.org/Team:Hong_Kong_HKUST><center><div id="kepala"><img src="https://static.igem.org/mediawiki/igem.org/c/c7/BANNER1_%281%29.png" style="height:121px;width:100%;"></div></center></a>
@@ Line 179: / Line 204: @@
 <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Wetlab">Wetlab</a>
 <ul>
-<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment">Experiments</a></li>
 <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/notebook">Notebook</a></li>
 <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/protocols">Protocols</a></li>
@@ Line 209: / Line 233: @@
 </div>
 <br><br><br>
-<div id="slide"><center><br><h3 class="title">Result</h3></center><br>
+<div id="slide"><center><br><h3 class="title">Result</h3></center><br></div>
 <div id="interview1"><h3>Experiment 1: Glyoxylate Shunt</h3><div id="art-img"><br><img src="https://static.igem.org/mediawiki/2013/c/c0/Expt3.png" style="padding-left:5px;width:90%;padding-top:5px;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment/exp4"><img src="http://w1ggroup.com/devere/img/more_off.png" style="width:80%;height:40px;padding-top:10px;"></a></center></div><div id="art-desc"><p id="art-desc-text"><br>Introduce glyoxylate enzymes responsible for the shunt</p></div></div>
@@ Line 216: / Line 240: @@
 <div id="presentation"><h3>Experiment 3: Fatty Acid Sensing Mechanism</h3><div id="pre-img"><br><img src="https://static.igem.org/mediawiki/2013/4/4f/Expt1.png" style="padding-left:5px;padding-top:5px;width:90%;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment/exp2"><img src="http://w1ggroup.com/devere/img/more_off.png" style="width:80%;height:40px;padding-top:10px;"></a></center></div><div id="pre-desc"><p id="pre-desc-text"><br>Introduce inducible system that allows tunable fatty acid uptake by sensing fatty acid concentration</p></div></div>
-<div id="article"><h3>Experiment 4: Cell Viability & Fatty Acid Quantification</h3><div id="pre-img"><img src="https://static.igem.org/mediawiki/2013/f/fa/Expt0.png" style="padding-left:2px;padding-top:5px;width:90%;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment/exp1"><img src="http://w1ggroup.com/devere/img/more_off.png" style="width:80%;height:40px;padding-top:10px;"></a></center></div><div id="pre-desc"><p id="pre-desc-text">Measuring cell viability at different fatty acid concentration & measure fatty acid uptake rate</p></div></div>
+<div id="article"><h3>Experiment 4: Cell Viability & Fatty Acid Quantification</h3><div id="pre-img"><br><img src="https://static.igem.org/mediawiki/2013/f/fa/Expt0.png" style="padding-left:2px;padding-top:5px;width:90%;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment/exp1"><img src="http://w1ggroup.com/devere/img/more_off.png" style="width:80%;height:40px;padding-top:10px;"></a></center></div><div id="pre-desc"><br><p id="pre-desc-text">Measuring cell viability at different fatty acid concentration & measure fatty acid uptake rate</p></div></div>
+<div id="slide1">
 <p id="yo">Our project was first proposed in mid-March and after a series of improvement and elaboration, we started our wet lab work from mid-June to late-September. After 3 months of memorable diligent work, we are proud to claim that we have achieved the results below:
 <ul>
    <li>Successfully constructed two parts</a> carrying mitochondrial leader sequence (MLS) compatible with RFC 10 and RFC 25 assembly standards respectively, (<a href="http://parts.igem.org/Part:BBa_K1119000">BBa_K1119000</a>, <a href="http://parts.igem.org/Part:BBa_K1119001">BBa_K1119001</a>).</li>
-   <li>Successfully constructed </a>parts containing the<i>aceA</i> and <i>aceB</i> elements of the glyoxylate shunt (<a href="http://parts.igem.org/Part:BBa_K1119002">BBa_K1119002</a>, <a href="http://parts.igem.org/Part:BBa_K1119003">BBa_K1119003</a>). </li>
+   <li>Successfully constructed </a>parts containing the <i>aceA</i> and <i>aceB</i> elements of the glyoxylate shunt (<a href="http://parts.igem.org/Part:BBa_K1119002">BBa_K1119002</a>, <a href="http://parts.igem.org/Part:BBa_K1119003">BBa_K1119003</a>). </li>
-   <li>Successfully constructed a part containing the constitutive regulatory promoter of Human Elongation Factor 1-alpha (<a href="http://parts.igem.org/Part:BBa_K1119004">BBa_K1119004</a>).</li>
+   <li>Successfully constructed a part containing the constitutive CMV promoter (<a href="http://parts.igem.org/Part:BBa_K1119006">BBa_K1119006</a>).</li>
-   <li>Successfully constructed a part containing the constitutive P<i>cmv</i> promoter (<a href="http://parts.igem.org/Part:BBa_K1119006">BBa_K1119006</a>).</li>
+   <li>Successfully characterized the constitutive CMV promoter in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119008">BBa_K1119008</a>).</li>
-  <li>Successfully characterized the constitutive promoter P<i>cmv</i> in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119008">BBa_K1119008</a>).</li>
+ <li>Successfully constructed a part containing the constitutive regulatory promoter of Human Elongation Factor 1-alpha (<a href="http://parts.igem.org/Part:BBa_K1119010">BBa_K1119010</a>).</li>
+<li>Successfully characterized the constitutive EF-1alpha promoter in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119011">BBa_K1119011</a>).</li>
    <li>Successfully characterized the mitochondrial leader sequence (MLS) in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119009">BBa_K1119009</a>).</li>
    <li>Successfully tested HepG2 cell viability in different sodium palmitate concentrations. </li>