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Exeter/10 July 2013
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== Morning == | == Morning == | ||
| - | - BBa_K322122 (cyan pigment) comes with the machinery to produce our pigment, albeit in low concentrations. 1ml of | + | - BBa_K322122 (cyan pigment) comes with the machinery to produce our pigment, albeit in low concentrations. 1ml of the BBa_K322122 liquid cultures from yesterday were transferred to eppendorf tubes and span down into a pellet at 13000 rpm for 5 minutes. All pellets were white, meaning further investigation is required to produce a colour. |
MiniPrep of the following Parts: | MiniPrep of the following Parts: | ||
Revision as of 10:24, 19 July 2013
Morning
- BBa_K322122 (cyan pigment) comes with the machinery to produce our pigment, albeit in low concentrations. 1ml of the BBa_K322122 liquid cultures from yesterday were transferred to eppendorf tubes and span down into a pellet at 13000 rpm for 5 minutes. All pellets were white, meaning further investigation is required to produce a colour.
MiniPrep of the following Parts:
- BBa_K322122, cyan pigment
- BBa_K592012, magenta pigment
- BBa_K592010, yellow pigment
Plasmids extracted are now in storage at -20°C.
Afternoon
Liquid cultures of the blue light module and magenta pigment
- BBa_K592010, yellow pigment
- BBa_K592012, magenta pigment
- BBa_B0015, a terminator
- BBa_K592005, FixJ protein intermediate
- BBa_K592004, blue light sensor
Retry of BBa_B0034 transformation
Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates!
(Next day, results of BBa_B0034 transformation)
We made two plates of BBa_B0034, each plate had 7 colonies. Our RFP control had 137 colonies.
