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| - | <li ><a href="/Team:SJTU-BioX-Shanghai/Project">Overview</a>
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| - | <li>
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| - | < li><a href="/ Team:SJTU-BioX-Shanghai/ Parts"> Parts</a ></li> | + | <font size=5><stong>?</strong></font> How to regulate an <strong>entire metabolic pathway</strong> <font style="font-style:italic;">in vivo</font>, delicately, <strong>accurately and conveniently</strong>, simultaneously controlling the expression of <strong>several</strong> genes? |
| - | <li><a href="/ Team:SJTU-BioX-Shanghai/ Prospect"> Prospect</a> | + | <br> |
| - | | + | <font size=5><stong>?</strong></font> And how to <strong>optimize metabolic fluxes so as to maximize desired products</strong>? |
| - | </li>
| + | <br><br> |
| - | <li><a href="/ Team:SJTU-BioX-Shanghai/ Team"> Team</a> | + | For decades, these questions have haunted “bioengineers”(especially synthetic biologists), who would like to have certain metabolites produced in living cells. |
| - | <ul>
| + | <br> |
| - | <li><a href="/ Team:SJTU-BioX-Shanghai/ Team/ members"> Members</a ></li> | + | The difficulty is again raised up when target genes endogenously reside on the <strong>genome</strong>, or have been implemented into the genome. |
| - | <li><a href="/ Team:SJTU-BioX-Shanghai/ Team"> Attributions</a></ li> | + | <br> |
| - | </ul>
| + | <br> |
| - | </li>
| + | So this year, our team, SJTU-BioX-Shanghai, is dedicated to solve the problem. We offer our product, the METABOLIC GEAR BOX, as a versatile BLACK BOX that can be conveniently applied in the optimization of whatever pathways. See <a href=" http://igem.bio-x.cn/Team:SJTU-BioX-Shanghai/ Project/ Applications/ Versatile#Tutorial/General_Protocol"> Tutorial/General Protocol (How to Use the Box)</a> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Notebook/protocol">Notebook</a>
| + | <br> |
| - | <ul>
| + | <br> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Notebook/protocol">Protocol</a>
| + | Inside the BOX, we integrate Luminous System, Light Sensors and CRISPRi. Luminous system and light sensors are used to address the problem of multiple gene regulation, while CRISPRi addresses the problem of genomic regulation. See <a href=" http://igem.bio-x.cn/Team:SJTU-BioX-Shanghai/ Project/ Overview#Motivation"> Problems</a> and <a href=" http://igem.bio-x.cn/Team:SJTU-BioX-Shanghai/ Project/ Overview#Inside_the_BOX"> Our Solutions</a> |
| - | </li>
| + | <br> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Notebook/Lab_log/July">Lablog</a>
| + | </div> |
| - | </li>
| + | </section> |
| - | </ul>
| + | <section class="row-padded"><div class="grid-container"><div class="grid-row row-4 "><div class="grid-unit col-3"><div id="pivot-1" class="pivot " bi:type="pivot"><h2 class="heading" bi:titleflag="t1" bi:title="t1">DIY！ ( *・ω・)✄</h2><div class="grid-row row-3 features"><div class="grid-unit"> |
| - | </li>
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| - | <li><a href="/Team:SJTU-BioX-Shanghai/Consideration/human">Human Practice</a>
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| - | <ul><li><a href="/Team:SJTU-BioX-Shanghai/Consideration/human">BioCraft</a></li>
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| - | < li><a href="/Team:SJTU-BioX-Shanghai/ Consideration/ human/ CC"> Communications on Campus</a>< /li> | + | <img bi:parenttitle="t1" src="/wiki/images/e/e7/13sjtudiy1.JPG" alt="How to make the box?" bi:mimiclink="true" bi:type="img" /> |
| - | < li><a href="/Team:SJTU-BioX-Shanghai/ Consideration/ human/RSB"> Reserch on Synthetic Biology</a> </li> | + | </div> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/ Consideration/human/ Cooperation"> Cooperation</a>< /li> | + | <div class=" highlight-text"> |
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| - | <li><a href="/Team:SJTU-BioX-Shanghai/Safety">Safety</a>
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| - | <ul><li><a href="/Team:SJTU-BioX-Shanghai/Safety">Safety Form</a></li>
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| - | <li><a href="/Team:SJTU-BioX-Shanghai/Safety/Security">Security</a></li>
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| - | < li><a href="/Team:SJTU-BioX-Shanghai/ Safety/ Environment"> Environment< /a></li> | + | |
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| - | <p><br>How to regulate an <strong>entire metabolic pathway</strong> <font style="font-style:italic;">in vivo</font>, delicately, <strong>accurately and conveniently</strong>, simultaneously controlling the expression of <strong>several</strong> genes? And how to <strong>optimize metabolic fluxes so as to maximize desired products</strong>? For decades, these questions remain haunting to “bioengineers”(especially synthetic biologists) who would like to have certain metabolites produced in live cells. The difficulty is again raised up when target genes endogenously reside on the <strong>genome</strong>, or have been implemented into the genome.<br></p>
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| - | <p><br>So this year, our team, SJTU-BioX-Shanghai, is dedicated to solve the problem. By integrating <strong>CRISPRi</strong>, the newly developed expression interference tool, with three <strong>light sensors</strong> (namely <font color=red>red light sensor</font>, <font color=green>green light sensor</font> and <font color=blue>blue light sensor</font>), we provide an <strong>easy-to-use BLACK BOX</strong>, a <strong>versatile</strong> platform on which researchers are able to quantitatively adjust the expression of any three enzymes in whatever pathway – to <strong>change the target, simply change the small guide RNA</strong> (sgRNA)!<br></p>
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| - | <p><br>In addition, with the <strong>luminous device and accompanying software</strong>, one could simply enter metabolomics data (catalytic rates of related enzymes) into our User Interface. The software will automatically conduct <strong>flux balance analysis (FBA)</strong>, giving out suggestions for optimal expression value of different enzymes. Then, the expression value would be converted into <strong>intensity of lights</strong> that are finally to be exerted on cell cultures. It is -- just convenient! :)<br></p>
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| - | <div id="entry-cover" style="background-image:url(/wiki/images/0/02/Luminous_System.png);">
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| - | <a href="/Team:SJTU-BioX-Shanghai/Project/Luminous_device/Design">
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| - | <p>The Luminous System produces red, green and blue lights of adjustable intensity into cell cultures.
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| - | <a href="/Team:SJTU-BioX-Shanghai/Project/Light_sensor">
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| - | <p><br><br/>Light Sensors transform optical signals into biological/chemical signals. </p>
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| - | <a href="/Team:SJTU-BioX-Shanghai/Project/Regulator">
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| - | <p><br/>CRISPRi receives biological/chemical signals from Light Sensors and responds by exerting proper interrogation on multiple gene targets</p></div></a
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| - | <div id="achievements-title"><p><strong style="color:rgb(102,102,255); font-size:60px;">A</strong>chievements</p></div>
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| - | <br><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4"><font face="Wingdings " size="4"></font><font face="Cambria, serif " size="4"> The <strong>BREAKTHROUGH</strong> we made:<strong> an easy-to-use BLACK BOX, a versatile platform</strong> for <strong>in vivo optimization of metabolic pathways</strong>.
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| - | <br><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The <strong>System</strong> we built: An wonderful <strong>Integration of Computer, Microcontroller Unit, Light Sources and their corresponding Sensors, and CRISPRi</strong>, a versatile gene interrogation tool. From </strong>digital to optical and finally to biological</strong>!
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| - | <br><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The Teams we Helped:</font></strong> Shared our parts with Zhejiang University and the Chinese University of Hong Kong. Helped with the modeling of NTU-Taida and NTU Taiwan. Assisted Shandong University establish their own team.
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| - | <br><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The Parts we submitted:</font></strong> CRISPRi and Light Sensors. Besides, we shared in our DIY section how to "manufacture" our luminous device, and we open-sourced our User Interface software.
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| - | <br><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The Application we proposed:</font></strong> Cooperate with companies, attempting to apply our project into biological industry.
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| - | <br><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> A Flaw we found:</font></strong> We helped the author of a JMB paper discover a mistaken unit for light intensity. <br><br>
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