Team:UNITN-Trento

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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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<a href="https://2013.igem.org/Team:UNITN-Trento/Extra/Judging" class="news">We got gold and 'Best Poster of the World' Award!! <img class="photo" src="https://static.igem.org/mediawiki/2013/b/b3/Tn-2013-Gold_award.png" /></a>
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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    <span class="silhouette">
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|[[Image:UNITN-Trento_logo.png|200px|right|frame]]
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        <img class="photo-1" src="https://static.igem.org/mediawiki/2013/a/a5/Tn-2013-silhouette.png">
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''Tell us more about your project. Give us background. Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:UNITN-Trento_team.png|right|frame|Your team picture]]
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            <p>Hi everybody, UniTN-Trento team is proud to introduce you <i>B. fruity</i>!</p>
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|align="center"|[[Team:UNITN-Trento | Team UNITN-Trento]]
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            <p><i>B. fruity</i> envisions a new environmental friendly way to control fruit ripening by exploiting an engineered, light-regulated strain of <i>B. subtilis</i>. The system works by synthesizing ethylene or methyl salicylate (MeSA) upon photoinduction.</p>
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<!--- The Mission, Experiments --->
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            <p><b>Ethylene</b> is a plant hormone widely used to ripen fruit. However its synthesis, handling and storage is expensive and dangerous. In contrast, <i>B. fruity</i> produces ethylene from inexpensive material by a metabolic intermediate: 2-oxoglutarate. Our system converts this metabolite to ethylene through the activity of an ethylene forming enzyme (EFE) from <i>Pseudomonas syringae</i>.</p>
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            <p><i>B. fruity</i> does not just accelerate ripening, but can also slow the process down, when desired, through the incorporation of a <b>methyl salicylate</b> (MeSA) synthesis pathway. MeSA was previously shown to inhibit the ripening of kiwifruit and tomatoes. The explored MeSA pathway builds upon the 2006 MIT iGEM project “<i>Eau de coli</i>”.</p>
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!align="center"|[[Team:UNITN-Trento|Home]]
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!align="center"|[[Team:UNITN-Trento/Team|Team]]
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            <p>As a proof of concept, we engineered <i>E. coli</i> with the above system plus the YF1/FixJ blue light receptor device.</p>
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=UNITN-Trento Official Team Profile]
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!align="center"|[[Team:UNITN-Trento/Project|Project]]
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            <p>We are hopeful that <i>B. fruity</i> will simplify the process of bringing fresh fruit from the field to the consumer.</p>
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!align="center"|[[Team:UNITN-Trento/Parts|Parts Submitted to the Registry]]
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        </div>
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!align="center"|[[Team:UNITN-Trento/Modeling|Modeling]]
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!align="center"|[[Team:UNITN-Trento/Notebook|Notebook]]
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!align="center"|[[Team:UNITN-Trento/Safety|Safety]]
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!align="center"|[[Team:UNITN-Trento/Attributions|Attributions]]
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<a href="https://2013.igem.org/Team:UNITN-Trento/Project/Ethylene#newGC" class="news-2">Check out our brand new results!!!</a>
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<a href="https://2013.igem.org/Team:UNITN-Trento/Project/Introduction#tour"></a>
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<a href="https://2013.igem.org/Team:UNITN-Trento/Project/Fruit%20ripening"></a>
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        <span class="tn-title">Achievements</span>
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        <img class="plasmid" src="https://static.igem.org/mediawiki/2013/b/b6/Tn-2013-home-ach-Plasmids.png" />
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<ul class="plasmid">
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<li>Built and submitted 16 New BioBricks;</li>
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<li>Successfully characterized 9 BioBricks;</li>
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<li>Improved 4 parts;</li>
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<img class="human" src="https://static.igem.org/mediawiki/2013/f/f9/Tn-2013-home-ach-Human-Practice_150.png" />
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<li>Hosted a high school student in the lab for the summer;</li>
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<li>Asked the opinion of local fruit consumers on our project;</li>
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<img class="product" src="https://static.igem.org/mediawiki/2013/f/fb/Tn-2013-home-ach-Products.png" />
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<li>Designed a fruit Vending Machine and a Home Edition <i>B. fruity</i> market product;</li>
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<img class="fruit" src="https://static.igem.org/mediawiki/2013/3/30/Tn-2013-home-ach-Fruit.png" />
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<ul class="fruit">
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<li>Ripened 5 bananas, 5 tomatoes, 3 kiwis, 2 plums, 2 apples;</li>
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<li>Eaten one banana bread, one plums crumble and one apple strudel. </li>
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<a href="https://2013.igem.org/Team:UNITN-Trento/Project/Vending%20Machine"></a>
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<a href="https://2013.igem.org/Team:UNITN-Trento/Extra/Fruit%20Info"></a>
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== Pages ==
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=== Templates ===
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Latest revision as of 11:32, 15 December 2013


We got gold and 'Best Poster of the World' Award!!

Hi everybody, UniTN-Trento team is proud to introduce you B. fruity!

B. fruity envisions a new environmental friendly way to control fruit ripening by exploiting an engineered, light-regulated strain of B. subtilis. The system works by synthesizing ethylene or methyl salicylate (MeSA) upon photoinduction.

Ethylene is a plant hormone widely used to ripen fruit. However its synthesis, handling and storage is expensive and dangerous. In contrast, B. fruity produces ethylene from inexpensive material by a metabolic intermediate: 2-oxoglutarate. Our system converts this metabolite to ethylene through the activity of an ethylene forming enzyme (EFE) from Pseudomonas syringae.

B. fruity does not just accelerate ripening, but can also slow the process down, when desired, through the incorporation of a methyl salicylate (MeSA) synthesis pathway. MeSA was previously shown to inhibit the ripening of kiwifruit and tomatoes. The explored MeSA pathway builds upon the 2006 MIT iGEM project “Eau de coli”.

As a proof of concept, we engineered E. coli with the above system plus the YF1/FixJ blue light receptor device.

We are hopeful that B. fruity will simplify the process of bringing fresh fruit from the field to the consumer.

Check out our brand new results!!!
Achievements
  • Built and submitted 16 New BioBricks;
  • Successfully characterized 9 BioBricks;
  • Improved 4 parts;
  • Hosted a high school student in the lab for the summer;
  • Asked the opinion of local fruit consumers on our project;
  • Designed a fruit Vending Machine and a Home Edition B. fruity market product;
  • Ripened 5 bananas, 5 tomatoes, 3 kiwis, 2 plums, 2 apples;
  • Eaten one banana bread, one plums crumble and one apple strudel.
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