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Team:DTU-Denmark/Notebook/7 July 2013
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==Main purpose== | ==Main purpose== | ||
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Make colony PCR on ''Nitrosomonas Europaea'' to extract the three AMO subunits. | Make colony PCR on ''Nitrosomonas Europaea'' to extract the three AMO subunits. | ||
Test which volume of liquid culture is the best. | Test which volume of liquid culture is the best. | ||
==Who was in the lab== | ==Who was in the lab== | ||
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Kristian | Kristian | ||
==Procedure== | ==Procedure== | ||
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*AMO1 = 1uL liquid culture | *AMO1 = 1uL liquid culture | ||
*AMO10 = 10uL liquid culture | *AMO10 = 10uL liquid culture | ||
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==Results== | ==Results== | ||
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See [[Team:DTU-Denmark/Notebook/8_July_2013#Pictures_from_gels|tomorrows gel]]. | See [[Team:DTU-Denmark/Notebook/8_July_2013#Pictures_from_gels|tomorrows gel]]. | ||
Revision as of 10:15, 26 July 2013
Contents |
208
Main purpose
Make colony PCR on Nitrosomonas Europaea to extract the three AMO subunits. Test which volume of liquid culture is the best.
Who was in the lab
Kristian
Procedure
- AMO1 = 1uL liquid culture
- AMO10 = 10uL liquid culture
- AMO20 = 20uL liquid culture
All reactions where made in triplicates 50uL reactions and mastermix was made as described in methods just with less MQ if template volume was over 1uL.
All tubes were run on standard PCR-program with 56°C annealing temp. and 2:00 extension time.
Results
See tomorrows gel.
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