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Team:UNITN-Trento
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| + | Hi everybody!<br/> | ||
| + | |||
| + | Our team is proud to introduce to you <i>B. fruity</i>, a new way to control the maturation of climateric and non-climateric fruit by engineering <i>Bacillus subtilis</i> with a light regulated genetic circuit that produces ethylene upon activation of inactive spores.<br/> | ||
| + | |||
| + | <b>Ethylene</b> is a plant hormone that is widely used to ripen fruit, such as bananas, kiwi, apples, and others. However, the chemical synthesis of ethylene is expensive and the handling and storage of the molecule is difficult and dangerous. <i>B. fruity</i> solves these problems by producing ethylene from simple, inexpensive starting material by hijacking a natural metabolic intermediate, 2-oxoglutarate from the TCA cycle, and converting this metabolite to ethylene through the activity of EFE (2-oxoglutarate oxygenase/decarboxylase), an enzyme from <i>Pseudomonas syringae</i>.<br/> | ||
| + | |||
| + | Moreover, <i>B. fruity</i> does not just accelerate maturation, <i>B. fruity</i> can also slow the process down, when desired, through the incorporation of a <b>methyl salicylate</b> (MeSA) synthesis pathway. MeSA was previously shown to inhibit the ripening of kiwi and tomato. The exploited MeSA pathway builds upon the xxxx (insert year) MIT iGEM project "<i>Eau de coli</i>".<br/> | ||
| + | |||
| + | We plan to build all of the needed pathways in both <i>E. coli</i> and <i>B. subtilis</i>. We are hopeful that <i>B. fruity</i> will simplify the process of bringing fresh fruit from the field to the consumer. | ||
| + | </html>}}<html> | ||
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Revision as of 13:46, 28 July 2013
This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
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Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs) | File:UNITN-Trento team.png Your team picture |
| Team UNITN-Trento |
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
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Hi everybody!
Our team is proud to introduce to you B. fruity, a new way to control the maturation of climateric and non-climateric fruit by engineering Bacillus subtilis with a light regulated genetic circuit that produces ethylene upon activation of inactive spores.
Ethylene is a plant hormone that is widely used to ripen fruit, such as bananas, kiwi, apples, and others. However, the chemical synthesis of ethylene is expensive and the handling and storage of the molecule is difficult and dangerous. B. fruity solves these problems by producing ethylene from simple, inexpensive starting material by hijacking a natural metabolic intermediate, 2-oxoglutarate from the TCA cycle, and converting this metabolite to ethylene through the activity of EFE (2-oxoglutarate oxygenase/decarboxylase), an enzyme from Pseudomonas syringae.
Moreover, B. fruity does not just accelerate maturation, B. fruity can also slow the process down, when desired, through the incorporation of a methyl salicylate (MeSA) synthesis pathway. MeSA was previously shown to inhibit the ripening of kiwi and tomato. The exploited MeSA pathway builds upon the xxxx (insert year) MIT iGEM project "Eau de coli".
We plan to build all of the needed pathways in both E. coli and B. subtilis. We are hopeful that B. fruity will simplify the process of bringing fresh fruit from the field to the consumer.
Our team is proud to introduce to you B. fruity, a new way to control the maturation of climateric and non-climateric fruit by engineering Bacillus subtilis with a light regulated genetic circuit that produces ethylene upon activation of inactive spores.
Ethylene is a plant hormone that is widely used to ripen fruit, such as bananas, kiwi, apples, and others. However, the chemical synthesis of ethylene is expensive and the handling and storage of the molecule is difficult and dangerous. B. fruity solves these problems by producing ethylene from simple, inexpensive starting material by hijacking a natural metabolic intermediate, 2-oxoglutarate from the TCA cycle, and converting this metabolite to ethylene through the activity of EFE (2-oxoglutarate oxygenase/decarboxylase), an enzyme from Pseudomonas syringae.
Moreover, B. fruity does not just accelerate maturation, B. fruity can also slow the process down, when desired, through the incorporation of a methyl salicylate (MeSA) synthesis pathway. MeSA was previously shown to inhibit the ripening of kiwi and tomato. The exploited MeSA pathway builds upon the xxxx (insert year) MIT iGEM project "Eau de coli".
We plan to build all of the needed pathways in both E. coli and B. subtilis. We are hopeful that B. fruity will simplify the process of bringing fresh fruit from the field to the consumer.
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