"

From 2013.igem.org

Revision as of 11:47, 31 July 2013 (view source) Batou (Talk | contribs) ← Older edit		Revision as of 11:53, 31 July 2013 (view source) Batou (Talk | contribs) Newer edit →
Line 10:		Line 10:
	<h2> Antibiotics </h2>		<h2> Antibiotics </h2>
		+	<b>Carbenicillin:</b><br>
		+
		+	<b>Kanamycin:</b><br>
		+
		+	<b>Chloramphenicol:</b><br>
		+
	<h2> LB and LB Agar </h2>		<h2> LB and LB Agar </h2>

---

Revision as of 11:53, 31 July 2013

Solutions, Media and Petri Dishes

Antibiotics

Carbenicillin:
Kanamycin:
Chloramphenicol:

LB and LB Agar

Mix 20 g of LB Broth in 1L of distilled water in a bottle of 1L, then put in autoclave.
Mix 17,5 g of LB Agar and in 500 mL of distilled water in a bottle of 1L, then put in autoclave.

CaCl2 and CaCl2+glycerol

Tris HCl

Disolve 4,6g of Trisbase in 30 mL of distilled water.
Adjust pH with concentrated HCl(~4 mL) until pH=7,5.
Add distilled water until 50 mL then put in autoclave.
If the solution has a yellow coloration, do the preparation again with better Trisbase.
Tris HCl is used to store DNA.