"

From 2013.igem.org

Revision as of 12:22, 31 July 2013 (view source) Batou (Talk | contribs) (u) ← Older edit		Revision as of 12:23, 31 July 2013 (view source) Batou (Talk | contribs) Newer edit →
Line 10:		Line 10:
	<h2> Antibiotics </h2>		<h2> Antibiotics </h2>
-	<b>Carbenicillin:</b><br>
-	<b>Kanamycin:</b><br>
-
-	<b>Chloramphenicol:</b><br>
-
-
-
-	<h2> Antibiotiques </h2>
	<b>Carbenicillin:</b> Put 150 mg of Carbenicilline in 15 mL of distilled water (10 mg per mL).<br>		<b>Carbenicillin:</b> Put 150 mg of Carbenicilline in 15 mL of distilled water (10 mg per mL).<br>
	Filter with a 0,22 µm millipore,divide in 2 mL tubes and store at -20 °C.<br>		Filter with a 0,22 µm millipore,divide in 2 mL tubes and store at -20 °C.<br>
Line 26:		Line 18:
	Filter with a 0,22 µm millipore,divide in 2 mL tubes and store at -20 °C.<br>		Filter with a 0,22 µm millipore,divide in 2 mL tubes and store at -20 °C.<br>
	Use 1 µL of Kanamycin for 1 mL of solution.<br><br>		Use 1 µL of Kanamycin for 1 mL of solution.<br><br>
-
	<b>Chloramphenicol:</b> Put 525 mg of Kanamycin in 15 mL of ethanol (35 mg per mL).<br>		<b>Chloramphenicol:</b> Put 525 mg of Kanamycin in 15 mL of ethanol (35 mg per mL).<br>

---

Revision as of 12:23, 31 July 2013

Solutions, Media and Petri Dishes

Antibiotics

Carbenicillin: Put 150 mg of Carbenicilline in 15 mL of distilled water (10 mg per mL).
Filter with a 0,22 µm millipore,divide in 2 mL tubes and store at -20 °C.
Use 1 µL of Carbenicillin for 1 mL of solution.

Kanamycin: Put 750 mg of Kanamycin in 15 mL of distilled water (50 mg per mL).
Filter with a 0,22 µm millipore,divide in 2 mL tubes and store at -20 °C.
Use 1 µL of Kanamycin for 1 mL of solution.

Chloramphenicol: Put 525 mg of Kanamycin in 15 mL of ethanol (35 mg per mL).
Filter with a 0,22 µm millipore,divide in 2 mL tubes and store at -20 °C.
Use 25 µL of Chloramphenicol for 1 mL of solution.

LB and LB Agar

Mix 20 g of LB Broth in 1L of distilled water in a bottle of 1L, then put in autoclave.
Mix 17,5 g of LB Agar and in 500 mL of distilled water in a bottle of 1L, then put in autoclave.

CaCl2 and CaCl2+glycerol

Tris HCl

Disolve 4,6g of Trisbase in 30 mL of distilled water.
Adjust pH with concentrated HCl(~4 mL) until pH=7,5.
Add distilled water until 50 mL then put in autoclave.
If the solution has a yellow coloration, do the preparation again with better Trisbase.
Tris HCl is used to store DNA.