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	Filter with a 0,22 µm millipore,divide in 2 mL tubes and store at -20 °C.<br>		Filter with a 0,22 µm millipore,divide in 2 mL tubes and store at -20 °C.<br>
	Use 25 µL of Chloramphenicol for 1 mL of solution.<br><br>		Use 25 µL of Chloramphenicol for 1 mL of solution.<br><br>
		+	Carbenicillin inhibit cell wall synthesis, kanamycin interfer with mRNA traduction and chloramphenicol inhibit ribosomial peptidyl transferase activity.

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Revision as of 12:32, 31 July 2013

Solutions, Media and Petri Dishes

Antibiotics

Carbenicillin: Put 150 mg of Carbenicilline in 15 mL of distilled water (10 mg per mL).
Filter with a 0,22 µm millipore,divide in 2 mL tubes and store at -20 °C.
Use 1 µL of Carbenicillin for 1 mL of solution.

Kanamycin: Put 750 mg of Kanamycin in 15 mL of distilled water (50 mg per mL).
Filter with a 0,22 µm millipore,divide in 2 mL tubes and store at -20 °C.
Use 1 µL of Kanamycin for 1 mL of solution.

Chloramphenicol: Put 525 mg of Kanamycin in 15 mL of ethanol (35 mg per mL).
Filter with a 0,22 µm millipore,divide in 2 mL tubes and store at -20 °C.
Use 25 µL of Chloramphenicol for 1 mL of solution.

Carbenicillin inhibit cell wall synthesis, kanamycin interfer with mRNA traduction and chloramphenicol inhibit ribosomial peptidyl transferase activity.

LB and LB Agar

Mix 20 g of LB Broth in 1L of distilled water in a bottle of 1L, then put in autoclave.
Mix 17,5 g of LB Agar and in 500 mL of distilled water in a bottle of 1L, then put in autoclave.

CaCl2 and CaCl2+glycerol

Tris HCl

Disolve 4,6g of Trisbase in 30 mL of distilled water.
Adjust pH with concentrated HCl(~4 mL) until pH=7,5.
Add distilled water until 50 mL then put in autoclave.
If the solution has a yellow coloration, do the preparation again with better Trisbase.
Tris HCl is used to store DNA.