Team:DTU-Denmark/Notebook/8 August 2013

From 2013.igem.org

(Difference between revisions)
(Gel on ON PCR samples)
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===Gel on ON PCR samples===
===Gel on ON PCR samples===
-
*Ladder
+
*1kb ladder
*Nir1
*Nir1
-
*Nir1 DM
+
*Nir1 5%DMSO
-
*Nir1 B1
+
*Nir1 1M Betaine
-
*Nir1 B3
+
*Nir1 3M Betaine
-
*Nir2 B3 U
+
*Nir2 3M Betaine U
-
*Nir2 B1 U
+
*Nir2 1M Betaine U
-
*Nir2 DM U
+
*Nir2 5%DMSO U
*Nir2 U
*Nir2 U
*Neg (Nir)
*Neg (Nir)
-
*Ref 0%
+
*Ref 0%DMSO
-
*Ref 2%
+
*Ref 2%DMSO
-
*Ref 5%
+
*Ref 5%DMSO
*AMO U
*AMO U
-
*AMO 2% U
+
*AMO 2%DMSO U
*Neg (AMO)
*Neg (AMO)
-
*NirG DMSO
+
*NirG 5%DMSO
*NirG
*NirG
-
*Ladder
+
*1kb ladder
[[File:2013-08-08 Nir.jpg| 600px]]
[[File:2013-08-08 Nir.jpg| 600px]]

Revision as of 20:45, 8 August 2013

8 August 2013

Contents

lab 208


Main purpose


Who was in the lab


Kristian, Julia, Henrike

Procedure


Results



Gel on yesterdays PCR

  • 1kb ladder
  • Yesterdays sample 1
  • Yesterdays sample 2
  • Yesterdays sample 3
  • Yesterdays sample 4
  • Neg.
  • Neg.
  • Nir2
  • cycAX Histag
  • 1kb ladder

2013-08-08 unkown gel.jpg


Gel on ON PCR samples

  • 1kb ladder
  • Nir1
  • Nir1 5%DMSO
  • Nir1 1M Betaine
  • Nir1 3M Betaine
  • Nir2 3M Betaine U
  • Nir2 1M Betaine U
  • Nir2 5%DMSO U
  • Nir2 U
  • Neg (Nir)
  • Ref 0%DMSO
  • Ref 2%DMSO
  • Ref 5%DMSO
  • AMO U
  • AMO 2%DMSO U
  • Neg (AMO)
  • NirG 5%DMSO
  • NirG
  • 1kb ladder

2013-08-08 Nir.jpg

Conclusion


1% agarose gel

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