Team:DTU-Denmark/Notebook/8 August 2013

From 2013.igem.org

(Difference between revisions)
(Gel on ON PCR samples)
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*1kb ladder
*1kb ladder
[[File:2013-08-08 Nir.jpg| 600px]]
[[File:2013-08-08 Nir.jpg| 600px]]
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 +
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===Gel on todays PCR samples===
 +
 +
*1kb ladder
 +
*Nir Q5 poly
 +
*Nir 2%DMSO Q5 poly
 +
*Nir 3M Betaine Q5 poly
 +
*Nir 5%DMSO Q5 poly
 +
*Nir1 Q5 poly
 +
*Nir1 2%DMSO Q5 poly
 +
*Nir1 5%DMSO Q5 poly
 +
*Nir1 3M Betaine Q5 poly
 +
*AMO USER primers
 +
*AMO +DMSO USER primers
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*AMO +Betaine USER primers
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*Nir1 DMSO
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*Nir1 Betaine
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*1kb ladder
 +
[[File:2013-08-08 nir1 AMO user.jpg| 600px]]
==Conclusion==
==Conclusion==
<hr/>
<hr/>
-
 
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1% agarose gel
 
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Revision as of 23:13, 8 August 2013

8 August 2013

Contents

lab 208


Main purpose


Who was in the lab


Kristian, Julia, Henrike

Procedure


Results



Gel on yesterdays PCR

  • 1kb ladder
  • Yesterdays sample 1
  • Yesterdays sample 2
  • Yesterdays sample 3
  • Yesterdays sample 4
  • Neg.
  • Neg.
  • Nir2
  • cycAX Histag
  • 1kb ladder

2013-08-08 unkown gel.jpg


Gel on ON PCR samples

  • 1kb ladder
  • Nir1
  • Nir1 5%DMSO
  • Nir1 1M Betaine
  • Nir1 3M Betaine
  • Nir2 3M Betaine U
  • Nir2 1M Betaine U
  • Nir2 5%DMSO U
  • Nir2 U
  • Neg (Nir)
  • Ref 0%DMSO
  • Ref 2%DMSO
  • Ref 5%DMSO
  • AMO U
  • AMO 2%DMSO U
  • Neg (AMO)
  • NirG 5%DMSO
  • NirG
  • 1kb ladder

2013-08-08 Nir.jpg


Gel on todays PCR samples

  • 1kb ladder
  • Nir Q5 poly
  • Nir 2%DMSO Q5 poly
  • Nir 3M Betaine Q5 poly
  • Nir 5%DMSO Q5 poly
  • Nir1 Q5 poly
  • Nir1 2%DMSO Q5 poly
  • Nir1 5%DMSO Q5 poly
  • Nir1 3M Betaine Q5 poly
  • AMO USER primers
  • AMO +DMSO USER primers
  • AMO +Betaine USER primers
  • Nir1 DMSO
  • Nir1 Betaine
  • 1kb ladder

2013-08-08 nir1 AMO user.jpg

Conclusion


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