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Small Phage
From 2013.igem.org
(Difference between revisions)
(→May) |
(→May) |
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| Line 15: | Line 15: | ||
- Commencement of spring!!! | - Commencement of spring!!! | ||
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- Discussed goals and outlined plans for spring term | - Discussed goals and outlined plans for spring term | ||
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: [[5.3 T7 phage amplification/purification]] | : [[5.3 T7 phage amplification/purification]] | ||
| - | + | ===5/4/13=== | |
| + | |||
| + | - Performed dilution series using stock 5.3 (-1 through -11) | ||
- Started two 5mL overnights of BL21 | - Started two 5mL overnights of BL21 | ||
| + | |||
| + | ===5/5/13=== | ||
| + | |||
| + | - Spot test using stock 5.3 and its dilution series (from 5.4) | ||
| + | |||
| + | - Started liquid culture for purification team (at around noon) | ||
| + | |||
| + | : 1mL of BL21 overnight + 4mL of LB + 100μL -2 phage (received stock) | ||
| + | |||
| + | - Started 5.5 amplification from a plaque test | ||
===5/20/13=== | ===5/20/13=== | ||
Revision as of 21:40, 21 May 2013
Contents |
March
April
May
5/1/13
LP, XL
- Commencement of spring!!!
- Discussed goals and outlined plans for spring term
5/2/13
LP, XL
- Designed primers for amplifying and sequencing phage capsid protein
- Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly
5/3/13
LP, XL
- Performed agar test, focusing primarily on ×8
- Processed phage amplification
5/4/13
- Performed dilution series using stock 5.3 (-1 through -11)
- Started two 5mL overnights of BL21
5/5/13
- Spot test using stock 5.3 and its dilution series (from 5.4)
- Started liquid culture for purification team (at around noon)
- 1mL of BL21 overnight + 4mL of LB + 100μL -2 phage (received stock)
- Started 5.5 amplification from a plaque test
5/20/13
LP, XL
- We performed T7 Mutagen Concentration Test
- We performed T7 Minor Capsid Protein PCR
June
July
August
September
