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From 2013.igem.org

Revision as of 22:25, 21 May 2013 (view source) Xiuqili (Talk | contribs) (→May) ← Older edit		Revision as of 22:28, 21 May 2013 (view source) Xiuqili (Talk | contribs) (→May) Newer edit →
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	- Went over procedure for applying mutagen and PCR with Dr. Grose		- Went over procedure for applying mutagen and PCR with Dr. Grose
		+
	- Performed spot tests under [[5.6 T7+ Liquid Culture Phage Concentration Test]]		- Performed spot tests under [[5.6 T7+ Liquid Culture Phage Concentration Test]]
		+
	- Started two 5mL BL21 overnights		- Started two 5mL BL21 overnights
		+
	- Learned how to create LB plates		- Learned how to create LB plates
		+
		+	===5/9/13===
		+
		+	- Practiced with ×6 and ×8 top agar
		+
		+	: [[5.9 T7 selection method test #2]]
		+
		+	- Started [[5.9 T7+ Liquid Culture Phage Concentration Test #2]]
		+
		+	- Discussed plans and schedule for next week.
	===5/20/13===		===5/20/13===

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Revision as of 22:28, 21 May 2013

• Home
• Team
  • Overview
  • Official Team Profile
  • Team Video
  • Undergraduates
  • Mentors
• Project
  • Overview
  • Background
  • Experimental Design
• Notebook
  • Overview
  • Phage Team
  • Cholera Team
• Results
  • Overview
  • Judging Criteria
  • Experimental
  • Modeling
  • Parts Submitted to the Registry
  • A Taste of Toronto
• Human Practices
  • Overview
  • Orem Public Library Visit
  • The Adventure of Baxter Bacteria
• Safety
• Attributions & Collaborations
  • Attributions & Acknowledgements
  • Collaborations
• iGEM 2013

Contents 1 March 2 April 3 May 3.1 5/1/13 3.2 5/2/13 3.3 5/3/13 3.4 5/4/13 3.5 5/5/13 3.6 5/6/13 3.7 5/7/13 3.8 5/8/13 3.9 5/9/13 3.10 5/20/13 4 June 5 July 6 August 7 September

March

April

May

5/1/13

LP, XL

- Commencement of spring!!!

- Discussed goals and outlined plans for spring term

5/2/13

LP, XL

- Designed primers for amplifying and sequencing phage capsid protein

- Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly

5/3/13

LP, XL

- Performed agar test, focusing primarily on ×8

5.3 T7 phage selection method test

- Processed phage amplification

5.3 T7 phage amplification/purification

5/4/13

XL

- Performed dilution series using stock 5.3 (-1 through -11)

- Started two 5mL overnights of BL21

5/5/13

XL

- Spot test using stock 5.3 and its dilution series (from 5.4)

5.3 T7 phage amplification/purification

- Started liquid culture for purification team (at around noon)

1mL of BL21 overnight + 4mL of LB + 100μL -2 phage (received stock)

- Started 5.5 amplification from a plaque test

5.5 Amplification from a plaque test

5/6/13

LP, XL

- Continued 5.3 T7 phage amplification/purification

- Performed liquid culture phage concentration test

5.6 T7+ Liquid Culture Phage Concentration Test

5/7/13

XL

- Started two 5mL of E coli BL21 overnight

- Designed procedure for applying mutagen and selecting for T7

5/8/13

- Went over procedure for applying mutagen and PCR with Dr. Grose

- Performed spot tests under 5.6 T7+ Liquid Culture Phage Concentration Test

- Started two 5mL BL21 overnights

- Learned how to create LB plates

5/9/13

- Practiced with ×6 and ×8 top agar

5.9 T7 selection method test #2

- Started 5.9 T7+ Liquid Culture Phage Concentration Test #2

- Discussed plans and schedule for next week.

5/20/13

LP, XL

- We performed T7 Mutagen Concentration Test

5.20 Mutagen Concentration Experiment

- We performed T7 Minor Capsid Protein PCR

5.20 T7 Minor Capsid Protein PCR

June

July

August

September