15/07/13

From 2013.igem.org

(Difference between revisions)
(Created page with "<div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;"> {| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border=...")
 
(2 intermediate revisions not shown)
Line 12: Line 12:
|}
|}
</div>
</div>
 +
 +
==Count colonies on agar plates==
 +
 +
{|border=1
 +
|-bgcolor=grey
 +
|Plate||Colonies
 +
|-
 +
|Viability control||Covered
 +
|-
 +
|Negative control||1 contaminant
 +
|-
 +
|20ul of 50pg/ul||135
 +
|-
 +
|20ul of 10pg/ul||96
 +
|-
 +
|200ul of 50pg/ul||~1600
 +
|-
 +
|200ul of 10pg/ul||944
 +
|}
 +
 +
Therefore there was 1.2x10^8 cfu/ug
 +
 +
 +
==Replate to get individual colonies==
 +
 +
*Take an individual colonies
 +
*Streak out onto new plate
 +
*Sterilize loop using bunsen burner
 +
*Incubate at 37 overnight

Latest revision as of 10:30, 14 August 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions

Count colonies on agar plates

PlateColonies
Viability controlCovered
Negative control1 contaminant
20ul of 50pg/ul135
20ul of 10pg/ul96
200ul of 50pg/ul~1600
200ul of 10pg/ul944

Therefore there was 1.2x10^8 cfu/ug


Replate to get individual colonies

  • Take an individual colonies
  • Streak out onto new plate
  • Sterilize loop using bunsen burner
  • Incubate at 37 overnight
Retrieved from "http://2013.igem.org/15/07/13"