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17/07/13
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| + | ==Isolating RFP plasmid== | ||
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| + | *Add 1.5ml of overnight culture to epindorph | ||
| + | *Centrifuge for 2mins | ||
| + | *Remove supernatent | ||
| + | *Repeat | ||
| + | *Use thermo scientific mini kit | ||
| + | *Measure concentration using nanodrop | ||
Revision as of 10:47, 14 August 2013
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Isolating RFP plasmid
- Add 1.5ml of overnight culture to epindorph
- Centrifuge for 2mins
- Remove supernatent
- Repeat
- Use thermo scientific mini kit
- Measure concentration using nanodrop
