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15/08/13
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| + | ==Serial dilution of herring sperm DNA== | ||
| + | |||
| + | *100ul DNA and 900ul TE to make master mix | ||
| + | *5 independent samples 10ul each were taken from master mix | ||
| + | **Each independent sample was diluted twice in 90ul of TE | ||
| + | *20 dilution samples were then nanodropped to check dilution accuracy | ||
| + | {|border=1 | ||
| + | |Sample nr||Concentration (ng/ul)||260/280||260/230 | ||
| + | |- | ||
| + | |nr. 1/1||529.9||1.81||2.26 | ||
| + | |- | ||
| + | |nr. 1/2||52.1||1.86||2.32 | ||
| + | |- | ||
| + | |nr. 2/1||454.3||1.81||2.28 | ||
| + | |- | ||
| + | |nr. 2/2||46||1.82||2.23 | ||
| + | |- | ||
| + | |nr. 3/1||457.3||1.83||2.28 | ||
| + | |- | ||
| + | |nr. 3/2||45.1||1.85||2.38 | ||
| + | |- | ||
| + | |nr. 4/1||522.8||1.82||2.27 | ||
| + | |- | ||
| + | |nr. 4/2||47.3||1.87||2.26 | ||
| + | |- | ||
| + | |nr. 5/1||481.4||1.82||2.28 | ||
| + | |- | ||
| + | |nr. 5/2||40.8||1.84||2.35 | ||
| + | |} | ||
| + | |||
| + | *Dilutions show accuracy | ||
| + | *Master dilution is sonicated and every 30mins a 30ul sample is taken to dilute twice (10ul of DNA in 90ul of TE buffer) | ||
| + | *6 30ul samples are taken | ||
| + | *Samples are then run on a gel | ||
Latest revision as of 13:29, 15 August 2013
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Serial dilution of herring sperm DNA
- 100ul DNA and 900ul TE to make master mix
- 5 independent samples 10ul each were taken from master mix
- Each independent sample was diluted twice in 90ul of TE
- 20 dilution samples were then nanodropped to check dilution accuracy
| Sample nr | Concentration (ng/ul) | 260/280 | 260/230 |
| nr. 1/1 | 529.9 | 1.81 | 2.26 |
| nr. 1/2 | 52.1 | 1.86 | 2.32 |
| nr. 2/1 | 454.3 | 1.81 | 2.28 |
| nr. 2/2 | 46 | 1.82 | 2.23 |
| nr. 3/1 | 457.3 | 1.83 | 2.28 |
| nr. 3/2 | 45.1 | 1.85 | 2.38 |
| nr. 4/1 | 522.8 | 1.82 | 2.27 |
| nr. 4/2 | 47.3 | 1.87 | 2.26 |
| nr. 5/1 | 481.4 | 1.82 | 2.28 |
| nr. 5/2 | 40.8 | 1.84 | 2.35 |
- Dilutions show accuracy
- Master dilution is sonicated and every 30mins a 30ul sample is taken to dilute twice (10ul of DNA in 90ul of TE buffer)
- 6 30ul samples are taken
- Samples are then run on a gel
