Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Exp/5.20 T7 Minor Capsid Protein PCR
From 2013.igem.org
(Difference between revisions)
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: - Visualize the gel in an Alphaimager. | : - Visualize the gel in an Alphaimager. | ||
- | 4) Sequencing | + | 4) Sequencing (5.29) |
- | + | : - Two PCR tubes were prepared | |
+ | :: iGem T7 F: 2uL of "5" PCR product + 1uL of forward primer (BI257) | ||
+ | :: iGem T7 R: 2uL of "5" PCR product + 1uL of reverse primer (BI258) | ||
'''V) Results''' | '''V) Results''' |
Revision as of 23:30, 29 May 2013
Small Phage Spring Notebook: Experiments
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5.20 T7 Minor Capsid Protein PCR
II) Expected Outcome
III) Reagents Used
IV) Procedure 1) Isolating DNA (5.20)
2) PCR (5.20)
3) Check with Agarose Electrophoresis (5.22)
4) Sequencing (5.29)
V) Results
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