29/08/13

From 2013.igem.org

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(Created page with "==Make 2% agarose gel== ==Run Tod operon ligation genes on the gel== ==Isolate the DNA from gel== *DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol")
 
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<div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;">
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{| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center"
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!align="center"|[[Team:Leicester|Home]]
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!align="center"|[[Team:Leicester/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]
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!align="center"|[[Team:Leicester/Project|Project]]
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!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Leicester/Modeling|Modeling]]
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!align="center"|[[Team:Leicester/Notebook|Notebook]]
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!align="center"|[[Team:Leicester/Safety|Safety]]
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!align="center"|[[Team:Leicester/Attributions|Attributions]]
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|}
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</div>
==Make 2% agarose gel==
==Make 2% agarose gel==
==Run Tod operon ligation genes on the gel==
==Run Tod operon ligation genes on the gel==
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*1.5h at 70V
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==Isolate the DNA from gel==
==Isolate the DNA from gel==
*DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol
*DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol
 +
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==Measuring concentration of DNA using nanodrop==
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{|border=1
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|Sample||Volume ul||Concentration ng/ul||260/280||260/230
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|-
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|A1||30||14.7||1.81||1.41
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|-
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|A2||31||13||1.71||1.43
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|-
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|B1||30||23.6||1.93||1.02
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|-
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|B2||29||21.9||1.88||1.33
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|-
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|C1||29||14.7||1.83||0.63
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|-
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|C2||30||15.5||1.86||0.64
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|}

Latest revision as of 11:29, 2 September 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions

Contents

Make 2% agarose gel

Run Tod operon ligation genes on the gel

  • 1.5h at 70V

Isolate the DNA from gel

  • DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol

Measuring concentration of DNA using nanodrop

SampleVolume ulConcentration ng/ul260/280260/230
A13014.71.811.41
A231131.711.43
B13023.61.931.02
B22921.91.881.33
C12914.71.830.63
C23015.51.860.64