Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Dailylog

From 2013.igem.org

(Difference between revisions)

Revision as of 21:32, 3 June 2013

Small Phage May - June Notebook: May 27 - June 9 Daily Log

5/28/13

- Started three 8mL E coli BL21 liquid culture at around 4pm.

5/29/13

- Continued 5.20 Mutagen Concentration Experiment

- Prepared sample for sequencing. This is done as part of 5.20 T7 Minor Capsid Protein PCR

- Dr. Studier responded to our email today! We also received the E coli stains containing plasmids with clone T7 genes. WE CAN START SITE DIRECTED MUTAGENESIS SOON!

5/30/13

- Plates from yesterday are taken out of incubation at around 4:00pm

5/31/13

- Discussed results for 5.20 Mutagen Concentration Experiment -> will need to try this one more time with minor adjustment to experimental design: plate -4 dilution using x6 and x8 top agar.

- Discussed plans for next week.

- Made new LB and x6 top agar.

6/2/13

- Made about 20ml of BL21 overnight

6/3/13

- Made new LB plates

- Plated -4 titer on x6 and x8 plates for 5.20 Mutagen Concentration Experiment

5/19/13

- Started two 5mL of E coli BL21 overnight

- Designed procedure for applying mutagen and selecting for T7

5/20/13

- Performed T7 Mutagen Concentration Test

5.20 Mutagen Concentration Experiment

- Performed T7 Minor Capsid Protein PCR

5.20 T7 Minor Capsid Protein PCR

5/21/13

- Started two 5mL E coli BL21 overnight at around 7:00pm

5/22/13

- Performed spot test for 5.20 Mutagen Concentration Experiment

- Ran agarose gel to confirm PCR product

5.20 T7 Minor Capsid Protein PCR

5/23/13

- Started two 25mL E coli BL21 liquid culture over night at around 6:00pm

5/24/13

- Proceeded with 5.20 Mutagen Concentration Experiment by performing preliminary selection using x8 top agar

5/25/13

- Took pictures in preparation for Progress Report

5/31/13

- Worked on transferring our notebook over to the iGEM wiki.

@@ Line 62: / Line 62: @@
 <br>
-<font size="4"> '''5/17/13''' </font>
+<font size="4"> '''6/2/13''' </font>
-- Determined that all LB plates from 5.8 had contamination
+- Made about 20ml of BL21 overnight
-- Poured new LB plates
-- Made x8 top agar
 <br>
-<font size="4"> '''5/18/13''' </font>
+<font size="4"> '''6/3/13''' </font>
-- Stacked up the LB plates made yesterday. No obvious sign of contamination seen.
+- Made new LB plates
-- Threw away the 5.8 LB plates (the ones with contamination).
+- Plated -4 titer on x6 and x8 plates for [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Exp/5.20 Mutagen Concentration Experiment|5.20 Mutagen Concentration Experiment]]
 <br>