Team:Peking/Project

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<h1 id="ProjectName">AROMATICS BUSTED</h1>
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                 <h1 id="ProjectSubname">A FAST, EASY AND ACCURATE METHOD TO DETECT TOXIC AROMATIC COMPOUNDS</h1>
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            <p id="AbstractContent">As aromatic pollution levels rise, and along with it the environmental and health hazards that it presents, finding a better way of detecting these aromatic compounds becomes more and more important. Some prokaryotes, including <I>Escherichia coli</I> and <I>Pseudomonas putida</I>, naturally produce proteins capable of both detecting toxic aromatic molecules and regulating the transcription of corresponding catabolic genes. However, these naturally existing biosensors are limited by their detection range, expression leakage, and induction ratio.<br/><br/>Using these proteins, our team designed a series of improved aromatic sensors and combined them with related enzymes from their original catabolic pathways to increase the molecule detection range of these sensors. In order to semi-quantitatively measure the concentrations of target compounds, we constructed a biological band-pass circuit, enabling our sensors to detect the concentrations of target compounds within a certain range. In sum, we have been working on a fast, easy and accurate way to detect toxic aromatic compounds.</p>
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Aromatic pollutants are becoming a worldwide concern. Monitoring aromatics in the environment, however, remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed <b>a comprehensive biosensors toolkit</b> to detect aromatics in the environment.
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Transcriptional regulators sensing each general class of aromatic compounds were first <a href="https://2013.igem.org/Team:Peking/Project/SensorMining"><b>bioinformatically determined</b></a> using the genomic data from prokaryotes, and then utilized to build <a href="https://2013.igem.org/Team:Peking/Project/BioSensors"><b>biosensor circuits</b></a> in living cells. Genetic tailoring such as promoter engineering was performed to tune their properties functionally. Most of these novel biosensors proved to be capable of faithfully sensing a specific group of aromatics. Furthermore, the <a href="https://2013.igem.org/Team:Peking/Project/BioSensors/MulticomponentAnalysis">synergistic/antagonistic effects</a> between their inducers was carefully examined; this allowed the combination of these biosensors to profile aromatics for the ease of practical applications.  
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In order to expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were gleaned from natural metabolic pathways, working as <a href="https://2013.igem.org/Team:Peking/Project/Plugins"><b>Adaptors</b></a> to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits. Additionally, for the ease of practical analysis, we have constructed a genetic device called the "<a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter"><b>Band-pass Filter</b></a>" to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter are able to robustly quantify the aromatics in environmental samples.
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In conclusion, Peking iGEM has <b>significantly enriched the repertoire of biosensors for aromatic compounds</b>. These novel biosensors, together with the Adaptors and the Band-pass Filter, will serve as intriguing synthetic biological tools for diverse practical applications, including pollution monitoring, bioremediation, and metabolic process control.
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Latest revision as of 18:12, 28 October 2013

Project Overview

Aromatic pollutants are becoming a worldwide concern. Monitoring aromatics in the environment, however, remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed a comprehensive biosensors toolkit to detect aromatics in the environment.

Transcriptional regulators sensing each general class of aromatic compounds were first bioinformatically determined using the genomic data from prokaryotes, and then utilized to build biosensor circuits in living cells. Genetic tailoring such as promoter engineering was performed to tune their properties functionally. Most of these novel biosensors proved to be capable of faithfully sensing a specific group of aromatics. Furthermore, the synergistic/antagonistic effects between their inducers was carefully examined; this allowed the combination of these biosensors to profile aromatics for the ease of practical applications.

In order to expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were gleaned from natural metabolic pathways, working as Adaptors to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits. Additionally, for the ease of practical analysis, we have constructed a genetic device called the "Band-pass Filter" to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter are able to robustly quantify the aromatics in environmental samples.

In conclusion, Peking iGEM has significantly enriched the repertoire of biosensors for aromatic compounds. These novel biosensors, together with the Adaptors and the Band-pass Filter, will serve as intriguing synthetic biological tools for diverse practical applications, including pollution monitoring, bioremediation, and metabolic process control.

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