Team:IIT Madras

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{{:Team:IIT Madras/Templates/head}}<html>
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{{:Team:IIT Madras/Templates/Header}}<html>
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<div class="container marketing">
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      <div id="myCarousel" class="carousel slide">
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      <ol class="carousel-indicators">
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        <li data-target="#myCarousel" data-slide-to="0" class="active"></li>
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          <img src="https://static.igem.org/mediawiki/2013/d/db/Iitm13_Slide_1.jpg" alt="Second slide" style="height: 500px; width: 100%; display: block;">
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          <div class="container">
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            <div class="carousel-caption">
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              <!--<h1>The Team</h1>
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              <p>Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p>
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              <p><a class="btn btn-large btn-primary" href="#">More info</a></p>-->
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<marquee behavior="scroll" direction="left" style="
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    color: black;
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    font-weight: bold;
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    font-size: 52px;
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">COMBATING SHIGA TOXIN</marquee>
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          <img src="https://static.igem.org/mediawiki/2013/f/f5/Iitm13_Slide_2.jpg" alt="Third slide" style="height: 500px; width: 100%; display: block;">
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              <h3>
 
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                Project Description
 
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              <p>
 
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                IIT Madras has had a very successful history at previous iGEM competitions. Shiga toxin is a deadly toxin secreted by Shigella dysenteriae and other shigatoxigenic bacteria. These bacteria inhabit the gastro-intestinal (GI) tract of cattle and produce a biofilm on the walls of the tract which is essential for the secretion of the toxin. According to statistics, more than 1 million people have been killed by the lethal toxin and many more have been infected. There is no established cure for this harmful toxin till date and this motivated us to come up with a feasible solution to this global problem using synthetic biology. Our idea mainly focuses on: Inhibiting biofilm production. Neutralizing the already produced toxin. For the first part, we have identified a compound, indole-3-acetaldehyde (I3A), which is a potent biofilm inhibitor.
 
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              <a href="#" class="button green more-info">
 
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                Read More
 
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          <img src="https://static.igem.org/mediawiki/2013/a/a7/Iitm13_Slide_3.jpg" alt="Third slide" style="height: 500px; width: 100%; display: block;">
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                <img src="http://www.advantagekarnataka.com/images/investment-sector/Inner-banners_Biotech.jpg">
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                <img src="https://fbcdn-sphotos-e-a.akamaihd.net/hphotos-ak-ash3/945977_532291323509340_604491180_n.jpg" alt="The Sort team">
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              Team!
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              Something about the iGEM IITM Team.
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              Branding??
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            <h2>
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              Updates
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            <marquee  behavior="scroll" direction="up">Am I going up?</marquee>
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          Shiga toxin, a worldwide menace, has killed over 1 million people to date and continues to afflict almost 150 million people each year. Currently, there is no treatment for Shiga toxicosis and it leads to complications in the human system like hemolytic uremic syndrome (HUS) and renal failure. Here, we propose a two-fold, novel synthetic biology approach to combat the lethal effect of the toxin. We aim to neutralize the already produced toxin through a nine amino acid Gb3 mimic peptide. We have engineered the Gb3 mimic along with a cellular export signal (ompF) downstream of AHL(quorum sensing molecule) inducible promoter (pLuxR). We also plan to prevent further toxin production by inhibiting the biofilm formation of shigatoxigenic E.coli using indole-3-acetaldehyde (I3A). We expect to validate our approach through functional assays and in silico modelling. Our findings can potentially initiate a new perspective of tackling Shiga toxicosis using synthetic biology tools.
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<img src="https://static.igem.org/mediawiki/2013/6/61/Iitm13_team_pic.JPG" width="100%" style="
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</html>{{:Team:IIT Madras/Templates/foot}}
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Latest revision as of 02:47, 28 September 2013

Indian Institute of Technology Madras
Shiga toxin, a worldwide menace, has killed over 1 million people to date and continues to afflict almost 150 million people each year. Currently, there is no treatment for Shiga toxicosis and it leads to complications in the human system like hemolytic uremic syndrome (HUS) and renal failure. Here, we propose a two-fold, novel synthetic biology approach to combat the lethal effect of the toxin. We aim to neutralize the already produced toxin through a nine amino acid Gb3 mimic peptide. We have engineered the Gb3 mimic along with a cellular export signal (ompF) downstream of AHL(quorum sensing molecule) inducible promoter (pLuxR). We also plan to prevent further toxin production by inhibiting the biofilm formation of shigatoxigenic E.coli using indole-3-acetaldehyde (I3A). We expect to validate our approach through functional assays and in silico modelling. Our findings can potentially initiate a new perspective of tackling Shiga toxicosis using synthetic biology tools.

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