Team:BYU Provo/Notebook/Phage Purification/Springexp/Period3/Dailylog

From 2013.igem.org

(Difference between revisions)
 
(8 intermediate revisions not shown)
Line 4: Line 4:
{| width="100%"
{| width="100%"
-
| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification May - June Notebook: May 27 - June 9 Daily Log'''</font>
+
| colspan="3" | <font color="#333399" size="5" font face="Calibri">'''Phage Purification May - June Notebook: May 27 - June 9 Daily Log'''</font>
<br>
<br>
Line 10: Line 10:
|- valign="top"
|- valign="top"
-
| style="width: 18%; background-color: transparent;"|
+
| style="width: 20%; background-color: transparent;"|
<font color="#333399" size="3" font face="Calibri">
<font color="#333399" size="3" font face="Calibri">
-
: [[Team:BYU_Provo/Phage_Purification|Overview]]
+
<font size = "4">
 +
 
 +
: <u> '''Phage Purification''' </u> </font>
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
Line 26: Line 28:
</font>
</font>
-
| style="width: 82%; background-color: transparent;"|
+
 
 +
 
 +
| style="width: 80%; background-color: transparent;"|
<font face="Calibri" size="3">
<font face="Calibri" size="3">
 +
 +
<br>
 +
<html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period2/Dailylog"><< Previous</a>
 +
 +
<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period4/Dailylog" style="display:block;float:right;">Next >></a>
 +
</html>
 +
 +
<br>
<font size="4"> '''5/29/13''' </font>
<font size="4"> '''5/29/13''' </font>
Line 66: Line 78:
<br>
<br>
-
<font size="4"> '''3/20/13''' </font>
+
<font size="4"> '''6/7/13''' </font>
 +
- Made 1 L of 10 X phage suspension buffer.
 +
: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.7 Phage Suspension Buffer Preparation|6.7 Phage Suspension Buffer Preparation]]
-
<br>
+
</font>
-
<font size="4"> '''3/22/13''' </font>
+
<html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period2/Dailylog"><< Previous</a>
 +
 +
<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period4/Dailylog" style="display:block;float:right;">Next >></a>
 +
</html>
-
<br>
 
-
 
-
<font size="4"> '''3/25/13''' </font>
 
-
 
-
 
-
<br>
 
-
 
-
<font size="4"> '''3/27/13''' </font>
 
-
 
-
 
-
<br>
 
-
 
-
<font size="4"> '''3/29/13''' </font>
 
-
 
-
 
-
<br>
 
-
 
-
</font>
 
|}
|}
{{TeamBYUProvoFooter}}
{{TeamBYUProvoFooter}}

Latest revision as of 00:03, 28 September 2013


Phage Purification May - June Notebook: May 27 - June 9 Daily Log



Phage Purification
March-April
May-June
July-August
September-October



<< Previous Next >>


5/29/13

- Finished the PEG purification.

5.26 PEG Purification


5/31/13

- Worked on setting up our notebook online.


6/3/13

- Ran the CsCl purification step.

6.3 CsCl Gradient Phage Purification

- Next class we will attempt extracting the phage from the gradient.


6/5/13

- Extracted the phage from 6.3 CsCl Gradient Phage Purification using a syringe and a needle.

Only extracted T4 because the T7 gradient was destroyed in the refrigerator.
Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed.
Placed these in the refrigerator.

- Prepared stocks of bacteria for future use.

6.5 Bacteria Cultures


6/7/13

- Made 1 L of 10 X phage suspension buffer.

6.7 Phage Suspension Buffer Preparation

<< Previous Next >>


Retrieved from "http://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Dailylog"