Team:BYU Provo/Notebook/Phage Purification/Springexp

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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage May - June Notebook'''</font>
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: '''Phage Purification May - June Notebook'''</font>
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: [[Team:BYU_Provo/Small_Phage|Overview]]
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: <u> '''Phage Purification''' </u> </font>
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
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<font size="5" font face="Calibri"> '''May 1 - May 12''' </font>
<font size="5" font face="Calibri"> '''May 1 - May 12''' </font>
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<font size="3" font face="Calibri"> This is the start of Spring Term at BYU! The weather is finally getting better: no more snowing. And we are ready to do some serious lab work! While we are waiting for our mutgen, 5-bromodeoxyuridine, to arrive, we decided to start this term with establishing the basic procedures for our direct evolution method. Specifically, this involves making sure that we can (1) grow and amplify T7 phage in liquid culture, (2) select for smaller phage using concentrated top agar, and (3) amplify phage from a single plaque. During these two weeks, we also designed primers for cloning and sequencing T7 capsid proteins as well as mapped out detailed procedures for both cloning T7 capsid proteins and applying mutagen. </font>
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<font size="3" font face="Calibri"> This semester we will are perfecting our procedures of purifying T4 and T7 bacteriophage through use of PEG and a CsCl gradient. During this week we began growing cultures to use and infect with phage to begin purification. We were held back waiting for materials, but were able to plan for our future procedures. </font>
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<font size="3" font face="Calibri"> In these two weeks, we performed our first round of mutagenesis and first PCR. Although we are still testing the results to optimize our protocol, we are optimistic about the outcome!</font>
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<font size="3" font face="Calibri"> We began performing titers to test the viability of phage after purification. We also ran a CsCl gradient, and started another round of PEG purification on T7 and T4 phage. </font>
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<font size="3" font face="Calibri"> We finished our CsCl gradient and have started a new round of phage purification to run through the CsCl gradient again. </font>
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<font size="3" font face="Calibri"> In these weeks, the CsCl gradients that we ran gave us an idea of the location of bands for both phage types, so that when mutated phage becomes available, we will be able to purify and extract it.We have also started a new round of PEG phage purification to run through the CsCl gradient again. </font>
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/PR| Progress Report]] </font>
 
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<font size="3" font face="Calibri"> In these two weeks, we worked on perfecting our techniques and preparing new samples to test. We purified more T7 phage using PEG purification, and it will be ready to run through CsCl gradients in the next two weeks.</font>
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period4/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period4/PR| Progress Report]] </font>
 
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<font size="3" font face="Calibri"> This week we finished a CsCl gradient of T7 phage. We successfully banded and extracted the phage. We performed a titer on the extracted phage and had a concentration high enough for an Electron Microscopy sample. We also prepared new bacteria to use with the large phage group. </font>
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period5/Explist|Experiment Listing]]
[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period5/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period5/PR| Progress Report]] </font>
 
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Latest revision as of 01:20, 28 September 2013


Phage Purification May - June Notebook



Phage Purification
March-April
May-June
July-August
September-October

<< Previous Next >>

May 1 - May 12


This semester we will are perfecting our procedures of purifying T4 and T7 bacteriophage through use of PEG and a CsCl gradient. During this week we began growing cultures to use and infect with phage to begin purification. We were held back waiting for materials, but were able to plan for our future procedures.


Daily log

Experiment Listing

Progress Report


BYUPPSpringIcon1.JPG


May 13 - May 26


We began performing titers to test the viability of phage after purification. We also ran a CsCl gradient, and started another round of PEG purification on T7 and T4 phage.


Daily log

Experiment Listing

Progress Report



Spring2.JPG


May 27 - June 9


In these weeks, the CsCl gradients that we ran gave us an idea of the location of bands for both phage types, so that when mutated phage becomes available, we will be able to purify and extract it.We have also started a new round of PEG phage purification to run through the CsCl gradient again.


Daily log

Experiment Listing



Archesutah4.jpg


June 10 - June 23


In these two weeks, we worked on perfecting our techniques and preparing new samples to test. We purified more T7 phage using PEG purification, and it will be ready to run through CsCl gradients in the next two weeks.


Daily log

Experiment Listing



Spring4.JPG
June 24 - June 30


This week we finished a CsCl gradient of T7 phage. We successfully banded and extracted the phage. We performed a titer on the extracted phage and had a concentration high enough for an Electron Microscopy sample. We also prepared new bacteria to use with the large phage group.


Daily log

Experiment Listing


<< Previous Next >>