Team:Goettingen/Safety

From 2013.igem.org

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===Navigation:===
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*[[Team:Goettingen/Project|Our Project]]
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**[[Team:Goettingen/Team/Reporter|Reporter Team]]
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**[[Team:Goettingen/Team/Array|Array Team]]
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**[[Team:Goettingen/Team/DAC|DAC Team]]
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*[[Team:Goettingen/Parts|Parts]]
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*<span style="color:#4a7ebb">Safety</span>
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===&nbsp;===
===&nbsp;===
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<br />
 
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<strong>Download as pdf</strong><br />
 
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<a href="https://static.igem.org/mediawiki/2013/6/65/Safety_Form_Goettingen.pdf"><img src="https://2013.igem.org/wiki/skins/common/images/icons/fileicon-pdf.png" /></a>
 
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<h2>Safety forms were approved on October 3rd, 2013 by the iGEM Safety Committee.</h2>
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<br />
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<br /><h3>Basic Safety Questions</h3>
<br /><h3>Basic Safety Questions</h3>
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== 1. Please describe the chassis organism(s) you will be using for this project. lf you will be using more than one chassis organism, provide information on each of them: ==
== 1. Please describe the chassis organism(s) you will be using for this project. lf you will be using more than one chassis organism, provide information on each of them: ==
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==3. List and describe all new or modified coding regions you will be using in your project. (If you use parts from the 2013 iGEM Distribution without modifying them, you do not need to list those parts.)==
==3. List and describe all new or modified coding regions you will be using in your project. (If you use parts from the 2013 iGEM Distribution without modifying them, you do not need to list those parts.)==
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   <td width="122" valign="top" style="width:91.75pt;border:solid black 1.0pt;
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   <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">Part number</span><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">.</span></p>
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   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
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  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
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  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
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  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
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  color:black;mso-font-kerning:0pt">Part number.<o:p></o:p></span></p>
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   <td width="165" valign="top" style="width:123.8pt;border:solid windowtext 1.0pt;
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   <td width="164" valign="top" style="width:123.1pt;border:solid black 1.0pt;
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   border-left:none;background:#99CCFF;padding:0pt 5.4pt 0pt 5.4pt">
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   mso-border-themecolor:text1;border-left:none;mso-border-left-alt:solid black .5pt;
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   <p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">Where
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  did you get the physical DNA for this part (which lab, synthesis &nbsp;company,
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  text1;background:#99CCFF;padding:0cm 5.4pt 0cm 5.4pt;height:66.3pt">
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  etc)</span></p>
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   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
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  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
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  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
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  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
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  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
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  color:black;mso-font-kerning:0pt">Where did you get the physical DNA for this
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  part (which <span class="SpellE">lab,synthesis</span> company, etc)<o:p></o:p></span></p>
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   <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">What pecies does this part
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  originally come from?</span></p>
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   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
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  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
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  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
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  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
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  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
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  color:black;mso-font-kerning:0pt">What species does this part originally come
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  from?<o:p></o:p></span></p>
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   <td width="94" valign="top" style="width:70.85pt;border:solid black 1.0pt;
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   <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">What is the Risk Group of the species?</span></p>
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   <p class="MsoNormal" align="center" style="text-align:center;line-height:115%;
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  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
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  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
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  color:black;mso-font-kerning:0pt">What is the Risk Group of the species?<o:p></o:p></span></p>
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   <td width="180" valign="top" style="width:135.0pt;border:solid windowtext 1.0pt;
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   <td width="195" valign="top" style="width:146.25pt;border:solid black 1.0pt;
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   border-left:none;background:#99CCFF;padding:0pt 5.4pt 0pt 5.4pt">
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   mso-border-themecolor:text1;border-left:none;mso-border-left-alt:solid black .5pt;
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   <p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">What
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  is the function of this part, in its parent species?</span></p>
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   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
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  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
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  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
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  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
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  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
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  color:black;mso-font-kerning:0pt">What is the function of this part, in its
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  parent species?<o:p></o:p></span></p>
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  mso-border-themecolor:text1;border-top:none;mso-border-top-alt:solid black .5pt;
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   <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">BBa_K1045000</span></p>
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   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
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  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
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  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
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  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
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  color:black;mso-font-kerning:0pt">BBa_K1045000<o:p></o:p></span></p>
   </td>
   </td>
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   <td width="165" valign="top" style="width:123.8pt;border-top:none;border-left:
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   <td width="164" valign="top" style="width:123.1pt;border-top:none;border-left:
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   none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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   none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
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   padding:0pt 5.4pt 0pt 5.4pt">
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  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
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   <p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">cloned,
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   solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
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   from purchased Mycobacterium genome</span></p>
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  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
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   <p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">&nbsp;</span></p>
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  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
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   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
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  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
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  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
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   mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
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  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
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  color:black;mso-font-kerning:0pt">Cloned from purchased <span class="SpellE">Mycobacteria</span>
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   genome<o:p></o:p></span></p>
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   <td width="110" valign="top" style="width:82.45pt;border-top:none;border-left:
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   none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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   none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
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   <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">Mycobacterium smegmatis</span></p>
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   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
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  mso-pagination:widow-orphan"><i><span lang="EN-US" style="font-size:10.0pt;
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  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
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  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
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  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
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  color:black;mso-font-kerning:0pt">Mycobacterium <span class="SpellE">smegmatis</span><o:p></o:p></span></i></p>
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   <td width="94" valign="top" style="width:70.85pt;border-top:none;border-left:
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   none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
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   padding:0pt 5.4pt 0pt 5.4pt">
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  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
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   <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">2</span></p>
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  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
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  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
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   <p class="MsoNormal" align="center" style="text-align:center;line-height:115%;
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  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
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  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
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  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
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  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
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  color:black;mso-font-kerning:0pt">2<o:p></o:p></span></p>
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   <td width="180" valign="top" style="width:135.0pt;border-top:none;border-left:
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   <td width="195" valign="top" style="width:146.25pt;border-top:none;border-left:
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   none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
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   padding:0pt 5.4pt 0pt 5.4pt">
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  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
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   <p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">code
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   regulatory protein DarR, which binds c-di-AMP.</span></p>
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  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">&nbsp;</span></p>
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  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
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   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
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   mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
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  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
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  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
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  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
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  color:black;mso-font-kerning:0pt">The operator DNA sequence that <span class="SpellE">DarR</span> binds<o:p></o:p></span></p>
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   </td>
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  <tr style="mso-yfti-irow:2;height:49.5pt">
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   <td width="122" valign="top" style="width:91.75pt;border:solid black 1.0pt;
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   padding:0pt 5.4pt 0pt 5.4pt">
+
  mso-border-themecolor:text1;border-top:none;mso-border-top-alt:solid black .5pt;
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   <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">BBa_K1045001</span></p>
+
   mso-border-top-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
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  text1;padding:0cm 5.4pt 0cm 5.4pt;height:49.5pt">
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   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
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  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
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  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
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  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
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  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
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  color:black;mso-font-kerning:0pt">BBa_K1045001<o:p></o:p></span></p>
   </td>
   </td>
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   <td width="165" valign="top" style="width:123.8pt;border-top:none;border-left:
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   <td width="164" valign="top" style="width:123.1pt;border-top:none;border-left:
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   none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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   none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
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   padding:0pt 5.4pt 0pt 5.4pt">
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  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
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   <p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">cloned,
+
   solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
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   from purchased Mycobacterium genome</span></p>
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  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
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   <p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">&nbsp;</span></p>
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  text1;padding:0cm 5.4pt 0cm 5.4pt;height:49.5pt">
 +
   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
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  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
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  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
   mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Cloned from purchased <span class="SpellE">Mycobacteria</span>
 +
   genome<o:p></o:p></span></p>
   </td>
   </td>
-
   <td width="173" valign="top" style="width:129.75pt;border-top:none;border-left:
+
   <td width="110" valign="top" style="width:82.45pt;border-top:none;border-left:
-
   none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
   none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
-
   padding:0pt 5.4pt 0pt 5.4pt">
+
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
-
   <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">Mycobacterium smegmatis</span></p>
+
   solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
-
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">&nbsp;</span></p>
+
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:49.5pt">
 +
   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><i><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Mycobacterium <span class="SpellE">smegmatis</span><o:p></o:p></span></i></p>
   </td>
   </td>
-
   <td width="91" valign="top" style="width:68.25pt;border-top:none;border-left:
+
   <td width="94" valign="top" style="width:70.85pt;border-top:none;border-left:
-
   none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
   none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
-
   padding:0pt 5.4pt 0pt 5.4pt">
+
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
-
   <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">2</span></p>
+
   solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:49.5pt">
 +
   <p class="MsoNormal" align="center" style="text-align:center;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">2<o:p></o:p></span></p>
   </td>
   </td>
-
   <td width="180" valign="top" style="width:135.0pt;border-top:none;border-left:
+
   <td width="195" valign="top" style="width:146.25pt;border-top:none;border-left:
-
   none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
   none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
-
   padding:0pt 5.4pt 0pt 5.4pt">
+
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
-
   <p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">the
+
   solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
-
   operator DNA sequence that DarR binds.</span></p>
+
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:49.5pt">
 +
   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Code regulatory protein <span class="SpellE">DarR</span>,
 +
   which binds c-<span class="SpellE">di</span>-AMP<o:p></o:p></span></p>
   </td>
   </td>
  </tr>
  </tr>
-
  <tr>
+
  <tr style="mso-yfti-irow:3;height:33.0pt">
-
   <td valign="top" style="border:solid windowtext 1.0pt;border-top:none;
+
   <td width="122" valign="top" style="width:91.75pt;border:solid black 1.0pt;
-
   padding:0pt 5.4pt 0pt 5.4pt">
+
  mso-border-themecolor:text1;border-top:none;mso-border-top-alt:solid black .5pt;
-
   <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">BBa_K1045002</span></p>
+
   mso-border-top-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">BBa_K1045002<o:p></o:p></span></p>
   </td>
   </td>
-
   <td width="165" valign="top" style="width:123.8pt;border-top:none;border-left:
+
   <td width="164" valign="top" style="width:123.1pt;border-top:none;border-left:
-
   none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
   none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
-
   padding:0pt 5.4pt 0pt 5.4pt">
+
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
-
   <p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">Cloned,
+
   solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
-
   from Bacillus genome</span></p>
+
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
   mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Cloned from Bacillus genome<o:p></o:p></span></p>
   </td>
   </td>
-
   <td width="173" valign="top" style="width:129.75pt;border-top:none;border-left:
+
   <td width="110" valign="top" style="width:82.45pt;border-top:none;border-left:
-
   none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
   none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
-
   padding:0pt 5.4pt 0pt 5.4pt">
+
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
-
   <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">Bacillus subtilis</span></p>
+
   solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><i><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Bacillus <span class="SpellE">subtilis</span><o:p></o:p></span></i></p>
   </td>
   </td>
-
   <td width="91" valign="top" style="width:68.25pt;border-top:none;border-left:
+
   <td width="94" valign="top" style="width:70.85pt;border-top:none;border-left:
-
   none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
   none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
-
   padding:0pt 5.4pt 0pt 5.4pt">
+
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
-
   <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">1</span></p>
+
   solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
   <p class="MsoNormal" align="center" style="text-align:center;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">1<o:p></o:p></span></p>
   </td>
   </td>
-
   <td width="180" valign="top" style="width:135.0pt;border-top:none;border-left:
+
   <td width="195" valign="top" style="width:146.25pt;border-top:none;border-left:
-
   none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
   none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
-
   padding:0pt 5.4pt 0pt 5.4pt">
+
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
-
   <p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">the
+
   solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
-
   c-di -AMP sensing riboswitch ydaO.</span></p>
+
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
   mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">The c-<span class="SpellE">di</span>-AMP
 +
  sensing <span class="SpellE">riboswitch</span> <span class="SpellE">YdaO</span><o:p></o:p></span></p>
   </td>
   </td>
  </tr>
  </tr>
-
  <tr>
+
  <tr style="mso-yfti-irow:4;height:49.5pt">
-
   <td valign="top" style="border:solid windowtext 1.0pt;border-top:none;
+
   <td width="122" valign="top" style="width:91.75pt;border:solid black 1.0pt;
-
   padding:0pt 5.4pt 0pt 5.4pt">
+
  mso-border-themecolor:text1;border-top:none;mso-border-top-alt:solid black .5pt;
-
   <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">BBa_K1045003</span></p>
+
   mso-border-top-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:49.5pt">
 +
   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">BBa_K1045003<o:p></o:p></span></p>
   </td>
   </td>
-
   <td width="165" valign="top" style="width:123.8pt;border-top:none;border-left:
+
   <td width="164" valign="top" style="width:123.1pt;border-top:none;border-left:
-
   none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
   none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
-
   padding:0pt 5.4pt 0pt 5.4pt">
+
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
-
   <p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">Cloned,
+
   solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
-
   from Listeria monocytogenes</span></p>
+
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:49.5pt">
 +
   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
   mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Cloned from Listeria monocytogenes genome<o:p></o:p></span></p>
   </td>
   </td>
-
   <td width="173" valign="top" style="width:129.75pt;border-top:none;border-left:
+
   <td width="110" valign="top" style="width:82.45pt;border-top:none;border-left:
-
   none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
   none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
-
   padding:0pt 5.4pt 0pt 5.4pt">
+
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
-
   <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">Listeria monocytogenes</span></p>
+
   solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:49.5pt">
 +
   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><i><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Listeria monocytogenes<o:p></o:p></span></i></p>
   </td>
   </td>
-
   <td width="91" valign="top" style="width:68.25pt;border-top:none;border-left:
+
   <td width="94" valign="top" style="width:70.85pt;border-top:none;border-left:
-
   none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
   none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
-
   padding:0pt 5.4pt 0pt 5.4pt">
+
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
-
   <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">2</span></p>
+
   solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:49.5pt">
 +
   <p class="MsoNormal" align="center" style="text-align:center;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">2<o:p></o:p></span></p>
   </td>
   </td>
-
   <td width="180" valign="top" style="width:135.0pt;border-top:none;border-left:
+
   <td width="195" valign="top" style="width:146.25pt;border-top:none;border-left:
-
   none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
   none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
-
   padding:0pt 5.4pt 0pt 5.4pt">
+
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
-
   <p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:Cambria">Diadenylate
+
   solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
-
  cyclase domain of L.monocytogenes, cdaA.</span></p>
+
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:49.5pt">
 +
   <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Diadenylate cyclase domain of L. monocytogenes,
 +
  <span class="SpellE">cdaA</span><o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:5;height:33.0pt">
 +
  <td width="122" valign="top" style="width:91.75pt;border:solid black 1.0pt;
 +
  mso-border-themecolor:text1;border-top:none;mso-border-top-alt:solid black .5pt;
 +
  mso-border-top-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">BBa_K1045004<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="164" valign="top" style="width:123.1pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Cloned from the Bacillus substilis genome<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="110" valign="top" style="width:82.45pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><i><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Bacillus substilis<o:p></o:p></span></i></p>
 +
  </td>
 +
  <td width="94" valign="top" style="width:70.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">1<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="195" valign="top" style="width:146.25pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span class="SpellE"><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:115%;
 +
  font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;mso-fareast-font-family:
 +
  &quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;mso-bidi-font-family:
 +
  &quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt">YdaO</span></span><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:
 +
  115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;
 +
  mso-fareast-font-family:&quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;
 +
  mso-bidi-font-family:&quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt">
 +
  Riboswitch with native Promoter and RBS<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:6;height:33.0pt">
 +
  <td width="122" valign="top" style="width:91.75pt;border:solid black 1.0pt;
 +
  mso-border-themecolor:text1;border-top:none;mso-border-top-alt:solid black .5pt;
 +
  mso-border-top-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">BBa_K1045005<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="164" valign="top" style="width:123.1pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Cloned from the Bacillus substilis genome<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="110" valign="top" style="width:82.45pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><i><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Bacillus substilis<o:p></o:p></span></i></p>
 +
  </td>
 +
  <td width="94" valign="top" style="width:70.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">1<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="195" valign="top" style="width:146.25pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span class="SpellE"><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:115%;
 +
  font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;mso-fareast-font-family:
 +
  &quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;mso-bidi-font-family:
 +
  &quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt">YdaO</span></span><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:
 +
  115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;
 +
  mso-fareast-font-family:&quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;
 +
  mso-bidi-font-family:&quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt">
 +
  Riboswitch with native RBS<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:7;height:33.0pt">
 +
  <td width="122" valign="top" style="width:91.75pt;border:solid black 1.0pt;
 +
  mso-border-themecolor:text1;border-top:none;mso-border-top-alt:solid black .5pt;
 +
  mso-border-top-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">BBa_K1045006<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="164" valign="top" style="width:123.1pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Cloned from the Bacillus substilis genome<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="110" valign="top" style="width:82.45pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><i><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Bacillus substilis<o:p></o:p></span></i></p>
 +
  </td>
 +
  <td width="94" valign="top" style="width:70.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">1<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="195" valign="top" style="width:146.25pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span class="SpellE"><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:115%;
 +
  font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;mso-fareast-font-family:
 +
  &quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;mso-bidi-font-family:
 +
  &quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt">YdaO</span></span><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:
 +
  115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;
 +
  mso-fareast-font-family:&quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;
 +
  mso-bidi-font-family:&quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt">
 +
  Riboswitch with native Promoter<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:8;height:33.0pt">
 +
  <td width="122" valign="top" style="width:91.75pt;border:solid black 1.0pt;
 +
  mso-border-themecolor:text1;border-top:none;mso-border-top-alt:solid black .5pt;
 +
  mso-border-top-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">BBa_K1045007<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="164" valign="top" style="width:123.1pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Cloned from the Bacillus substilis genome<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="110" valign="top" style="width:82.45pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><i><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Bacillus substilis<o:p></o:p></span></i></p>
 +
  </td>
 +
  <td width="94" valign="top" style="width:70.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">1<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="195" valign="top" style="width:146.25pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span class="SpellE"><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:115%;
 +
  font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;mso-fareast-font-family:
 +
  &quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;mso-bidi-font-family:
 +
  &quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt">YdaO</span></span><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:
 +
  115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;
 +
  mso-fareast-font-family:&quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;
 +
  mso-bidi-font-family:&quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt">
 +
  Riboswitch only<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:9;height:16.5pt">
 +
  <td width="122" valign="top" style="width:91.75pt;border:solid black 1.0pt;
 +
  mso-border-themecolor:text1;border-top:none;mso-border-top-alt:solid black .5pt;
 +
  mso-border-top-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:16.5pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">BBa_K1045009<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="164" valign="top" style="width:123.1pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:16.5pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">From 2013 distribution kit<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="110" valign="top" style="width:82.45pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:16.5pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span class="SpellE"><i><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:115%;
 +
  font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;mso-fareast-font-family:
 +
  &quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;mso-bidi-font-family:
 +
  &quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt">E.coli</span></i></span><i><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:
 +
  115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;
 +
  mso-fareast-font-family:&quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;
 +
  mso-bidi-font-family:&quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt"><o:p></o:p></span></i></p>
 +
  </td>
 +
  <td width="94" valign="top" style="width:70.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:16.5pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">1<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="195" valign="top" style="width:146.25pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:16.5pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Terminator<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:10;height:16.5pt">
 +
  <td width="122" valign="top" style="width:91.75pt;border:solid black 1.0pt;
 +
  mso-border-themecolor:text1;border-top:none;mso-border-top-alt:solid black .5pt;
 +
  mso-border-top-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:16.5pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">BBa_K1045010<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="164" valign="top" style="width:123.1pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:16.5pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">From 2013 distribution kit<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="110" valign="top" style="width:82.45pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:16.5pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span class="SpellE"><i><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:115%;
 +
  font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;mso-fareast-font-family:
 +
  &quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;mso-bidi-font-family:
 +
  &quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt">E.coli</span></i></span><i><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:
 +
  115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;
 +
  mso-fareast-font-family:&quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;
 +
  mso-bidi-font-family:&quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt"><o:p></o:p></span></i></p>
 +
  </td>
 +
  <td width="94" valign="top" style="width:70.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:16.5pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">1<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="195" valign="top" style="width:146.25pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:16.5pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span class="SpellE"><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:115%;
 +
  font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;mso-fareast-font-family:
 +
  &quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;mso-bidi-font-family:
 +
  &quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt">Ribsome</span></span><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:
 +
  115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;
 +
  mso-fareast-font-family:&quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;
 +
  mso-bidi-font-family:&quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt">
 +
  binding site<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:11;height:16.5pt">
 +
  <td width="122" valign="top" style="width:91.75pt;border:solid black 1.0pt;
 +
  mso-border-themecolor:text1;border-top:none;mso-border-top-alt:solid black .5pt;
 +
  mso-border-top-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:16.5pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">BBa_K1045011<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="164" valign="top" style="width:123.1pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:16.5pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">From 2013 distribution kit<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="110" valign="top" style="width:82.45pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:16.5pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span class="SpellE"><i><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:115%;
 +
  font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;mso-fareast-font-family:
 +
  &quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;mso-bidi-font-family:
 +
  &quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt">E.coli</span></i></span><i><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:
 +
  115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;
 +
  mso-fareast-font-family:&quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;
 +
  mso-bidi-font-family:&quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt"><o:p></o:p></span></i></p>
 +
  </td>
 +
  <td width="94" valign="top" style="width:70.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:16.5pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">1<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="195" valign="top" style="width:146.25pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:16.5pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Promoter<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:12;height:33.0pt">
 +
  <td width="122" valign="top" style="width:91.75pt;border:solid black 1.0pt;
 +
  mso-border-themecolor:text1;border-top:none;mso-border-top-alt:solid black .5pt;
 +
  mso-border-top-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">BBa_K1045012<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="164" valign="top" style="width:123.1pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Assembled with BBa_K1045000 and BBa_J23110<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="110" valign="top" style="width:82.45pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt"></td>
 +
  <td width="94" valign="top" style="width:70.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt"></td>
 +
  <td width="195" valign="top" style="width:146.25pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt"></td>
 +
</tr>
 +
<tr style="mso-yfti-irow:13;height:33.0pt">
 +
  <td width="122" valign="top" style="width:91.75pt;border:solid black 1.0pt;
 +
  mso-border-themecolor:text1;border-top:none;mso-border-top-alt:solid black .5pt;
 +
  mso-border-top-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">BBa_K1045013<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="164" valign="top" style="width:123.1pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span class="SpellE"><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:115%;
 +
  font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;mso-fareast-font-family:
 +
  &quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;mso-bidi-font-family:
 +
  &quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt">Aseembled</span></span><span lang="EN-US" style="font-size:10.0pt;mso-bidi-font-size:11.0pt;line-height:
 +
  115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;mso-ascii-theme-font:major-latin;
 +
  mso-fareast-font-family:&quot;Arial Unicode MS&quot;;mso-hansi-theme-font:major-latin;
 +
  mso-bidi-font-family:&quot;Arial Unicode MS&quot;;color:black;mso-font-kerning:0pt">
 +
  with<span style="mso-spacerun:yes">&nbsp; </span>BBa_K1045012 and BBa_E0240 <o:p></o:p></span></p>
 +
  </td>
 +
  <td width="110" valign="top" style="width:82.45pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt"></td>
 +
  <td width="94" valign="top" style="width:70.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt"></td>
 +
  <td width="195" valign="top" style="width:146.25pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt"></td>
 +
</tr>
 +
<tr style="mso-yfti-irow:14;height:33.0pt">
 +
  <td width="122" valign="top" style="width:91.75pt;border:solid black 1.0pt;
 +
  mso-border-themecolor:text1;border-top:none;mso-border-top-alt:solid black .5pt;
 +
  mso-border-top-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">BBa_K1045014<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="164" valign="top" style="width:123.1pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Assembled with BBa_K1045011 and
 +
  BBa_K1045013, <o:p></o:p></span></p>
 +
  </td>
 +
  <td width="110" valign="top" style="width:82.45pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt"></td>
 +
  <td width="94" valign="top" style="width:70.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt"></td>
 +
  <td width="195" valign="top" style="width:146.25pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt"></td>
 +
</tr>
 +
<tr style="mso-yfti-irow:15;height:33.0pt">
 +
  <td width="122" valign="top" style="width:91.75pt;border:solid black 1.0pt;
 +
  mso-border-themecolor:text1;border-top:none;mso-border-top-alt:solid black .5pt;
 +
  mso-border-top-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">BBa_K1045015<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="164" valign="top" style="width:123.1pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt">
 +
  <p class="MsoNormal" align="left" style="text-align:left;line-height:115%;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;
 +
  mso-bidi-font-size:11.0pt;line-height:115%;font-family:&quot;Cambria&quot;,&quot;serif&quot;;
 +
  mso-ascii-theme-font:major-latin;mso-fareast-font-family:&quot;Arial Unicode MS&quot;;
 +
  mso-hansi-theme-font:major-latin;mso-bidi-font-family:&quot;Arial Unicode MS&quot;;
 +
  color:black;mso-font-kerning:0pt">Assembled with<span style="mso-spacerun:yes">&nbsp; </span>BBa_K1045014, BBa_K1045010 and
 +
  BBa_E0240<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="110" valign="top" style="width:82.45pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
  solid black .5pt;mso-border-top-themecolor:text1;mso-border-left-alt:solid black .5pt;
 +
  mso-border-left-themecolor:text1;mso-border-alt:solid black .5pt;mso-border-themecolor:
 +
  text1;padding:0cm 5.4pt 0cm 5.4pt;height:33.0pt"></td>
 +
  <td width="94" valign="top" style="width:70.85pt;border-top:none;border-left:
 +
  none;border-bottom:solid black 1.0pt;mso-border-bottom-themecolor:text1;
 +
  border-right:solid black 1.0pt;mso-border-right-themecolor:text1;mso-border-top-alt:
 +
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 +
  color:black;mso-font-kerning:0pt">BBa_K1045016<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="164" valign="top" style="width:123.1pt;border-top:none;border-left:
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 +
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 +
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 +
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 +
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 +
  color:black;mso-font-kerning:0pt">Assembled with BBa_K1045001 and
 +
  BBa_K1045009, <o:p></o:p></span></p>
 +
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 +
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 +
  color:black;mso-font-kerning:0pt">BBa_K1045017<o:p></o:p></span></p>
 +
  </td>
 +
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 +
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 +
  color:black;mso-font-kerning:0pt">Assembled with BBa_K1045015 and
 +
  BBa_K1045016<o:p></o:p></span></p>
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===Satefy Questions Part 2, ''Listeria monocytogenes''===
 +
 +
Those questions are answered because we have parts originated from organisms from Risk Group 2. They are ''Listeria monocytogenes'' and ''Mycobacterium smegmatis''.
 +
 +
==1. Organism name and strain name or number.==
 +
 +
''Listeria monocytogenes''
 +
 +
==2. Organism Risk Group:==
 +
 +
2
 +
 +
==3. If you are using this organism as a chassis, write "chassis". If you are using a genetic part from the organism, give the name of the part and a brief description of what it does and why you are using it.==
 +
 +
BBa_K1045003  Diadenylate cyclase domain of L. monocytogenes, DacA (CdaA, Lmo2120) We would like to look into diadenylate cyclase(DAC) of gram-positive pathogen, as potential target for novel antibiotics.
 +
 +
==4. How did you physically acquire the organism or part?==
 +
 +
Cloned from purchased Listeria genome
 +
 +
==5. What potential safety/health risks to team members, other people at your institution, or the general public could arise from your use of this organism/part?==
 +
 +
We actually didn't work with ''Listeria monocytogenes''. Our parts were cloned from purchased genome. And the parts themselves are not hazardous nor toxic since mammals and a few gram-negative bacteria don't use c-di-AMP as a signal molecule.
 +
 +
==6. What measures do you intend to take to ensure that your project is safe for team members, other people at your institution, and the general public?==
 +
 +
We follow strictly the S1 protocol to avoid any risks.
 +
 +
==7. If you are using only a part from the organism, and you believe the part by itself is not dangerous, explain why you believe it is not dangerous.==
 +
 +
This part is non-hazardous nor toxic while mammals and gram-negative bacteria don't use c-di-AMP as a signal molecule. And this is the reason why c-di-AMP is a possible target for novel antibiotics
 +
 +
==8. Why do you need to use this organism/part? Is there an organism/part from a less dangerous Risk Group that would accomplish the same purpose?==
 +
 +
We would like to look into diadenylate cyclase(DAC) of gram-positive pathogen, as potential target for novel antibiotics. There are definitely DAC in less dangerous organism like B.substilis, but DAC from B.substilis is rather difficult to purify, and our final goal is to get a 3D structure.
 +
 +
==9. Is the organism/part listed under the Australia Group guidelines, or otherwise restricted for transport? If so, how will your team ship this part to iGEM and the Jamborees?==
 +
 +
No.
 +
 +
==10. Please describe the BioSafety Level of the lab in which the team works, or description of safety features of lab (Refer to Basic Safety form, question 8. d.). If you are using organisms with a BSL level greater than you lab, please explain any additional safety precautions you are taking.==
 +
 +
BSL1
 +
 +
 +
 +
 +
 +
===Satefy Questions Part 2, ''Mycobacterium smegmatis''===
 +
 +
==1. Organism name and strain name or number.==
 +
 +
''Mycobacterium smegmatis''
 +
 +
==2. Organism Risk Group:==
 +
 +
2
 +
 +
==3. If you are using this organism as a chassis, write "chassis". If you are using a genetic part from the organism, give the name of the part and a brief description of what it does and why you are using it.==
 +
 +
BBa_K1045000  The operator DNA sequence that DarR binds, DarR operator
 +
 +
BBa_K1045001  Code regulatory protein DarR, which binds c-di-AMP
 +
 +
DarR is reported to be a transcriptional inhibitor in Mycobacterium smegmatis and c-di-AMP stimulates its binding to its operator sequence, acting as a co-inhibitor. (Zhang ''et al.'', 2012) We have built a reporter system of c-di-AMP based on that.
 +
 +
==4. How did you physically acquire the organism or part?==
 +
 +
Cloned from purchased Mycobacteria genome
 +
 +
==5. What potential safety/health risks to team members, other people at your institution, or the general public could arise from your use of this organism/part?==
 +
 +
We actually didn't work with ''Mycrobacterium segmatic''. Our parts were cloned from purchased genome. And the parts themselves are not hazardous nor toxic.
 +
 +
==6. What measures do you intend to take to ensure that your project is safe for team members, other people at your institution, and the general public?==
 +
 +
We follow strictly the S1 protocol to avoid any risks.
 +
 +
==7. If you are using only a part from the organism, and you believe the part by itself is not dangerous, explain why you believe it is not dangerous.==
 +
 +
BBa_K1045000 is only  a 14bp palindrome operator sequence.
 +
 +
BBa_K1045001 encodes  a transcriptional inhibitor DarR. DarR has no catalytic function and is not toxic.
 +
 +
==8. Why do you need to use this organism/part? Is there an organism/part from a less dangerous Risk Group that would accomplish the same purpose?==
 +
 +
Because DarR is the very recently discovered regulatory protein which responds to c-di-AMP.There is no another protein in a less dangerous organism discovered yet.
 +
 +
==9. Is the organism/part listed under the Australia Group guidelines, or otherwise restricted for transport? If so, how will your team ship this part to iGEM and the Jamborees?==
 +
 +
No.
 +
 +
==10. Please describe the BioSafety Level of the lab in which the team works, or description of safety features of lab (Refer to Basic Safety form, question 8. d.). If you are using organisms with a BSL level greater than you lab, please explain any additional safety precautions you are taking.==
 +
 +
BSL1
==Faculty Advisor Name:==
==Faculty Advisor Name:==
-
<p>Jörg Stülke</p>
+
 
 +
Jörg Stülke
 +
 
 +
 
 +
 
 +
 
==&nbsp;==
==&nbsp;==
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Latest revision as of 12:27, 18 October 2013





The beast and its Achilles heel:

 A novel target to fight multi-resistant pathogenic bacteria



Safety forms were approved on October 3rd, 2013 by the iGEM Safety Committee.




Basic Safety Questions

1. Please describe the chassis organism(s) you will be using for this project. lf you will be using more than one chassis organism, provide information on each of them:

Species

Strain no/name

Risk Group

Risk group source link

Disease risk to humans? If so, which disease?

E.coli (K12)

XL1 blue

1

www.absa.org/riskgroups/bacteria

search.php?genus=&species=coli

Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.

E.coli (K12)

DH5 alpha

1

www.absa.org/riskgroups/bacteria

search.php?genus=&species=coli

Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.

E.coli(B)

T7 express(NEB)

1

www.absa.org/riskgroups/bacteria

search.php?genus=&species=coli

Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.

E.coli(B)

BL21

1

www.absa.org/riskgroups/bacteria

search.php?genus=&species=coli

Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.

2. Highest Risk Group Listed:

Risk group 1

3. List and describe all new or modified coding regions you will be using in your project. (If you use parts from the 2013 iGEM Distribution without modifying them, you do not need to list those parts.)

Part number.

Where did you get the physical DNA for this part (which lab,synthesis company, etc)

What species does this part originally come from?

What is the Risk Group of the species?

What is the function of this part, in its parent species?

BBa_K1045000

Cloned from purchased Mycobacteria genome

Mycobacterium smegmatis

2

The operator DNA sequence that DarR binds

BBa_K1045001

Cloned from purchased Mycobacteria genome

Mycobacterium smegmatis

2

Code regulatory protein DarR, which binds c-di-AMP

BBa_K1045002

Cloned from Bacillus genome

Bacillus subtilis

1

The c-di-AMP sensing riboswitch YdaO

BBa_K1045003

Cloned from Listeria monocytogenes genome

Listeria monocytogenes

2

Diadenylate cyclase domain of L. monocytogenes, cdaA

BBa_K1045004

Cloned from the Bacillus substilis genome

Bacillus substilis

1

YdaO Riboswitch with native Promoter and RBS

BBa_K1045005

Cloned from the Bacillus substilis genome

Bacillus substilis

1

YdaO Riboswitch with native RBS

BBa_K1045006

Cloned from the Bacillus substilis genome

Bacillus substilis

1

YdaO Riboswitch with native Promoter

BBa_K1045007

Cloned from the Bacillus substilis genome

Bacillus substilis

1

YdaO Riboswitch only

BBa_K1045009

From 2013 distribution kit

E.coli

1

Terminator

BBa_K1045010

From 2013 distribution kit

E.coli

1

Ribsome binding site

BBa_K1045011

From 2013 distribution kit

E.coli

1

Promoter

BBa_K1045012

Assembled with BBa_K1045000 and BBa_J23110

BBa_K1045013

Aseembled with  BBa_K1045012 and BBa_E0240

BBa_K1045014

Assembled with BBa_K1045011 and BBa_K1045013,

BBa_K1045015

Assembled with  BBa_K1045014, BBa_K1045010 and BBa_E0240

BBa_K1045016

Assembled with BBa_K1045001 and BBa_K1045009,

BBa_K1045017

Assembled with BBa_K1045015 and BBa_K1045016

4. Do the biological materials used in your lab work pose any of the following risks? Please describe.

a. Risks to the safety and health of team members or others working in the lab?

The organism we used in the lab E.coli (K12) DH5 alpha, could be pathogenicto human beings. lt may cause irritation to skin, eyes, and respiratorytract and affect kidneys.

b. Risks to the safety and health of the general public, if released by design or by accident?

The E.coli strain we used do have pathogenicity to human. But the relatively low pathogenicity, short life and vulnerability to environment factors ofthe used strain have limited the risk to general public.

c. Risks to the environment, if released by design or by accident?

The E.coli strains we used during the project are transformed with plasmids containing antibiotic markers The release of those transformed strains may cause problems like the leakage of resistance genes to wild bacterial flora.

d. Risks to security through malicious misuse by individuals, groups, or countries?

The strains we used during our project do not contain hazardous genetic compartments. The risk through malicious misuse is quite low

5. If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise? (Consider the different categories of risks that are listed in parts a-d of the previous question.) Also, what risks might arise if the knowledge you generate or the methods you develop became widely available? (Note: This is meant to be a somewhat open-ended discussion question.)

The system we are trying to build is mainly meant for drug screening in pharmaceutical industry. The scale of production will always be limited.

6. Does your project include any design features to address safety risks? (For example: kill switches, auxotrophic chassis, etc.) Note that including such features is not mandatory to participate in iGEM, but many groups choose to include them.

No. But as the most used model organism in molecular biology, the risk of E.coli is relatively low.

7. What safety training have you received (or plan to receive in the future)? Provide a brief description, and a link to your institution’s safety training requirements, if available.

Before the project started, we all received instructions concerning the safety problems, such as how to protect ourselves in the lab, how to handle the biological materials properly to lower the risk to public and environment.

8. Under what biosafety provisions will / do you work?

a. Please provide a link to your institution biosafety guidelines.

www.uni-goettingen.de/en/401.html
(this website has links to all safety concerning documents, but all in german)

b. Does your institution have an Institutional Biosafety Committee, or an equivalent group? If yes, have you discussed your project with them? Describe any concerns they raised with your project, and any changes you made to your project planbased on their review.

www.uni-goettingen.de/de/401.html

c. Does your country have national biosafety regulations or guidelines? If so, please provide a link to these regulations or guidelines if possible.

www.baua.de/en/Homepage.html

d. According to the WHO Biosafety Manual, what is the BioSafety Level rating of your lab? (Check the summary table on page 3, and the fuller description that starts on page 9.) If your lab does not fit neatly into category 1, 2, 3, or 4, please describe its safety features [see 2013.igem.org/Safety for help].

The biosafety level of our lab is Categroy 1.

e. What is the Risk Group of your chassis organism(s), as you stated in question 1? If it does not match the BSL rating of your laboratory, please explain what additional safety measures you are taking.

Risk Group 1.



Satefy Questions Part 2, Listeria monocytogenes

Those questions are answered because we have parts originated from organisms from Risk Group 2. They are Listeria monocytogenes and Mycobacterium smegmatis.

1. Organism name and strain name or number.

Listeria monocytogenes

2. Organism Risk Group:

2

3. If you are using this organism as a chassis, write "chassis". If you are using a genetic part from the organism, give the name of the part and a brief description of what it does and why you are using it.

BBa_K1045003 Diadenylate cyclase domain of L. monocytogenes, DacA (CdaA, Lmo2120) We would like to look into diadenylate cyclase(DAC) of gram-positive pathogen, as potential target for novel antibiotics.

4. How did you physically acquire the organism or part?

Cloned from purchased Listeria genome

5. What potential safety/health risks to team members, other people at your institution, or the general public could arise from your use of this organism/part?

We actually didn't work with Listeria monocytogenes. Our parts were cloned from purchased genome. And the parts themselves are not hazardous nor toxic since mammals and a few gram-negative bacteria don't use c-di-AMP as a signal molecule.

6. What measures do you intend to take to ensure that your project is safe for team members, other people at your institution, and the general public?

We follow strictly the S1 protocol to avoid any risks.

7. If you are using only a part from the organism, and you believe the part by itself is not dangerous, explain why you believe it is not dangerous.

This part is non-hazardous nor toxic while mammals and gram-negative bacteria don't use c-di-AMP as a signal molecule. And this is the reason why c-di-AMP is a possible target for novel antibiotics

8. Why do you need to use this organism/part? Is there an organism/part from a less dangerous Risk Group that would accomplish the same purpose?

We would like to look into diadenylate cyclase(DAC) of gram-positive pathogen, as potential target for novel antibiotics. There are definitely DAC in less dangerous organism like B.substilis, but DAC from B.substilis is rather difficult to purify, and our final goal is to get a 3D structure.

9. Is the organism/part listed under the Australia Group guidelines, or otherwise restricted for transport? If so, how will your team ship this part to iGEM and the Jamborees?

No.

10. Please describe the BioSafety Level of the lab in which the team works, or description of safety features of lab (Refer to Basic Safety form, question 8. d.). If you are using organisms with a BSL level greater than you lab, please explain any additional safety precautions you are taking.

BSL1



Satefy Questions Part 2, Mycobacterium smegmatis

1. Organism name and strain name or number.

Mycobacterium smegmatis

2. Organism Risk Group:

2

3. If you are using this organism as a chassis, write "chassis". If you are using a genetic part from the organism, give the name of the part and a brief description of what it does and why you are using it.

BBa_K1045000 The operator DNA sequence that DarR binds, DarR operator

BBa_K1045001 Code regulatory protein DarR, which binds c-di-AMP

DarR is reported to be a transcriptional inhibitor in Mycobacterium smegmatis and c-di-AMP stimulates its binding to its operator sequence, acting as a co-inhibitor. (Zhang et al., 2012) We have built a reporter system of c-di-AMP based on that.

4. How did you physically acquire the organism or part?

Cloned from purchased Mycobacteria genome

5. What potential safety/health risks to team members, other people at your institution, or the general public could arise from your use of this organism/part?

We actually didn't work with Mycrobacterium segmatic. Our parts were cloned from purchased genome. And the parts themselves are not hazardous nor toxic.

6. What measures do you intend to take to ensure that your project is safe for team members, other people at your institution, and the general public?

We follow strictly the S1 protocol to avoid any risks.

7. If you are using only a part from the organism, and you believe the part by itself is not dangerous, explain why you believe it is not dangerous.

BBa_K1045000 is only a 14bp palindrome operator sequence.

BBa_K1045001 encodes a transcriptional inhibitor DarR. DarR has no catalytic function and is not toxic.

8. Why do you need to use this organism/part? Is there an organism/part from a less dangerous Risk Group that would accomplish the same purpose?

Because DarR is the very recently discovered regulatory protein which responds to c-di-AMP.There is no another protein in a less dangerous organism discovered yet.

9. Is the organism/part listed under the Australia Group guidelines, or otherwise restricted for transport? If so, how will your team ship this part to iGEM and the Jamborees?

No.

10. Please describe the BioSafety Level of the lab in which the team works, or description of safety features of lab (Refer to Basic Safety form, question 8. d.). If you are using organisms with a BSL level greater than you lab, please explain any additional safety precautions you are taking.

BSL1

Faculty Advisor Name:

Jörg Stülke



 

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