Team:BYU Provo/Notebook/Phage Purification/Springexp/Period4/Dailylog
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- | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification May - June Notebook: | + | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification May - June Notebook: June 10 - June 23 Daily Log'''</font> |
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+ | : <u> '''Phage Purification''' </u> </font> | ||
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]] | : [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]] | ||
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+ | <br> | ||
+ | <html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Dailylog"><< Previous</a> | ||
+ | |||
+ | <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period5/Dailylog" style="display:block;float:right;">Next >></a> | ||
+ | </html> | ||
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+ | <br> | ||
<font size="4"> '''6/10/13''' </font> | <font size="4"> '''6/10/13''' </font> | ||
- | - Prepared RNase for future use. | + | - Prepared RNase A for future use. |
- | + | :[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10RNasePreparation|6.10 RNase A Preparation]] | |
- | + | - Prepared T4 and T7 cultures. | |
+ | :[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10PhagePreparation|6.10 Phage Preparation]] | ||
<br> | <br> | ||
- | <font size="4"> '''6/ | + | <font size="4"> '''6/12/13''' </font> |
- | - | + | - Began first step of purification process using phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10PhagePreparation|6.10 Phage Preparation]]. |
- | + | : [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.12PEGPurification|6.12 PEG Purification]] | |
<br> | <br> | ||
- | <font size="4"> '''6/ | + | <font size="4"> '''6/14/13''' </font> |
- | - | + | - Worked on updating the website. |
- | + | <br> | |
+ | |||
+ | <font size="4"> '''6/17/13''' </font> | ||
+ | |||
+ | - Set up CsCl gradient | ||
+ | [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.17CsClGradient|6.17 CsCl Gradient]] | ||
+ | |||
+ | - Prepared T4 and T7 cultures. | ||
+ | |||
+ | :[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period4/Exp/6.17PhagePreparation|6.17 Phage Preparation]] | ||
- | |||
<br> | <br> | ||
- | <font size="4"> '''6/ | + | <font size="4"> '''6/19/13''' </font> |
- | - | + | - Began first step of purification process using phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period4/Exp/6.17PhagePreparation|6.17 Phage Preparation]]. |
- | : | + | |
- | + | : [[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period1/Exp/6.19PEGPurification|6.19 PEG Purification]] | |
- | : | + | |
+ | - Ran spot tests on collected band from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.17CsClGradient|6.17 CsCl Gradient]]. | ||
+ | |||
+ | :[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period4/Exp/6.19Spot Test|6.19 Spot Test]] | ||
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- | |||
<br> | <br> | ||
- | <font size="4"> '''6/ | + | <font size="4"> '''6/20/13''' </font> |
- | + | - Finished [[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period1/Exp/6.19PEGPurification|6.19 PEG Purification]] by centrifuging solution at 10,000 g 15 minutes at 4<sup>◦</sup> C, removing supernatant, and suspending the pellet in phage suspension buffer. | |
- | + | ||
+ | <br> | ||
+ | |||
+ | <font size="4"> '''6/21/13''' </font> | ||
+ | |||
+ | We centrifuged the pellet at 10,000 g for 15 min at 4<sup>◦</sup> C after suspending it to clean up the walls of the tube. | ||
+ | |||
+ | <br> | ||
</font> | </font> | ||
+ | |||
+ | <html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Dailylog"><< Previous</a> | ||
+ | |||
+ | <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period5/Dailylog" style="display:block;float:right;">Next >></a> | ||
+ | </html> | ||
+ | |||
|} | |} | ||
{{TeamBYUProvoFooter}} | {{TeamBYUProvoFooter}} |
Latest revision as of 00:04, 28 September 2013
Phage Purification May - June Notebook: June 10 - June 23 Daily Log
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6/10/13 - Prepared RNase A for future use. - Prepared T4 and T7 cultures.
6/12/13 - Began first step of purification process using phage from 6.10 Phage Preparation.
6/14/13 - Worked on updating the website.
6/17/13 - Set up CsCl gradient 6.17 CsCl Gradient - Prepared T4 and T7 cultures.
6/19/13 - Began first step of purification process using phage from 6.17 Phage Preparation. - Ran spot tests on collected band from 6.17 CsCl Gradient.
6/20/13 - Finished 6.19 PEG Purification by centrifuging solution at 10,000 g 15 minutes at 4◦ C, removing supernatant, and suspending the pellet in phage suspension buffer.
6/21/13 We centrifuged the pellet at 10,000 g for 15 min at 4◦ C after suspending it to clean up the walls of the tube.
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