Team:UNITN-Trento/Parts

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Latest revision as of 15:48, 4 October 2013

In this beautiful page you can find the fruits of our summer’s efforts, otherwise you can just go to the official page.

The number of bananas indicates:

: This is an intermediate part and it has not been characterized.
: the part does not work, due to a mistake of some kind, or we do not know if it works and more tests must be performed.
: the part was characterized but doesn't work always as expected.
: the part works as expected and has been characterized.

♫ ♪ “One banana, two bananas, three bananas, four. Four bananas make a bunch and so do many more.” ♪ ♫

[cit. The Banana Splits – The Tra La La Song]

Ethylene

	BBa_K1065000	EFE
	Ethylene Forming Enzyme gene (EFE) that forms ethylene starting from 2-oxoglutarate.

	BBa_K1065002	EFE with double-terminator
	EFE followed by the double terminator BBa_B0015.

	BBa_K1065001	EFE arabinose-regulated
	This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.

Methyl salicylate

	BBa_K1065100	BSMT1
	This part encodes BSMT1, an enzyme that methylates salycilic acid to obtain methyl salycilate. It is the original part from the 'Eau de coli' MIT iGEM 2006 project, re-submitted (and resurrected) by us in pSB1C3.

	BBa_K1065103	PchBA
	PchA is the enzyme responsible for the formation of isochorismate from chorismate. The next step is performed by the PchB enzyme, which converts isochorismate in salicylic acid. This is another part from the 'Eau de coli' MIT iGEM 2006 project that we re-submitted (and resurrected) in pSB1C3.

	BBa_K1065104	SAM synthetase
	This part encodes the SAM (S-AdoMet) synthetase gene, which we friendly call "uncle SAM". SAM is a methyl donor required to produce MeSA and other methylated molecules.

	BBa_K1065101	BSMT1 arabinose-regulated
	A methyltransferase (BSMT1) regulated by the araCpBAD promoter.

	BBa_K1065105	SAM synthetase under pLac promoter
	This is SAM synthetase (uncle SAM) regulated by the constitutive Lac promoter.

	BBa_K1065102	PchBA under pLac and BSMT1 arabinose-regulated
	The wintergreen device: PchBA under a Lac promoter followed by BSMT1 under the araCpBAD promoter.

	BBa_K1065106	BSMT1, PchBA and SAM synthetase
	An improved version of the wintergreen device: BBa_K1065102 with SAM synthetase to boost production.

Blue light

	BBa_K1065302	Blue light circuit to produce amilGFP
	A device to characterize our improved part BBa_K1065305 controlled by pLac promoter.

	BBa_K1065305	BBa_K952003 improved
	No rbs, no party!!!!We improved BBa_K952003 by mutagenesis to add the missing RBS, now the part works.

	BBa_K1065309	Blue light ethylene producing device
	Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE.

	BBa_K1065310	Complete blue-light sensing circuit with an inverter
	Yf1 and FixJ are regulated by the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced.

	BBa_K1065311	Complete blue-light sensing device with inverter, amilCP and EFE
	Our complete circuit composed by:J23100, Yf1 and FixJ, pFixK2, CI, pLambda, amilCP and EFE.

	BBa_K1065306	J23100+Yf1+FixJ
	Yf1 (photosensor) and FixJ (response regulator) under the Anderson promoter (BBa_J23100).

	BBa_K1065307	J23100+Yf1+FixJ (with terminators)
	The same as BBa_K1065306, just with the terminators at the end. This part was not submitted to the registry. If you are interested please contact us.

	BBa_K1065308	BBa_K1065307 with inverter
	This is an intermediate part to build a blue light inducible genetic circuit with the inverter. This part is in pSB1A2 and it was not submitted to the registry. If you are interested in this part please contact us.

	BBa_K1065303	Yf1+FixJ+pFixK2
	The starting point to build a blue-light sensing circuit: the photosensor (Yf1) and the response regulator (FixJ) with its promoter (pFixK2). This part was build merging BBa_K592016 and BBa_K592006.

	BBa_K1065300	Blue light circuit to produce amilGFP
	It does not work beacuse it lacks the RBS before the amilGFP gene.

Bacillus subtilis

	BBa_K1065201	pXyl+GFP
	A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840).

	BBa_K1065203	Efe+Bba_B0015 in pSpac.
	EFE in pSpac vector, specific for B. subtilis.

	BBa_K1065204	pXyl+EFE
	EFE in pXyl vector, specific for B. subtilis.

[http://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Parts&action=edit Edit this page] | Main Page

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 <div class="tn-parts-container">
-     <div class="menu">
+     <div class="legend">
+        <div class="sheet">
+            <p>
+                In this beautiful page you can find <i>the fruits</i> of our summer’s efforts, otherwise you can just go to the <a href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&group=UNITN-Trento" target="_blank">official page</a>.
+            <p>
+                The number of bananas indicates:
+            </p>
+            <ul>
+                <li><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />: This is an intermediate part and it has not been characterized.</li>
+                <li><img src="https://static.igem.org/mediawiki/2013/1/1e/Tn-2013-parts-Banana-green-parts.png" />: the part does not work, due to a mistake of some kind, or we do not know if it works and more tests must be performed. </li>
+                <li><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />: the part was characterized but doesn't work always as expected.</li>
+                <li><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />: the part works as expected and has been characterized.</li>
+            </ul>
+            <p style="margin-bottom: 0;">
+                <i>&#9835; &#9834; “One banana, two bananas, three bananas, four. Four bananas make a bunch and so do many more.” &#9834; &#9835;</i>
+            </p>
+            <p style="margin-top: 0; text-align: right;">
+                [cit. The Banana Splits – <i>The Tra La La Song</i>]
+            </p>
+        </div>
      </div>
      <div class="content">
@@ Line 18: / Line 36: @@
              <div id="label1" class="label">
              </div>
-             <span class="title">Ethylene</span>
+             <span class="title"><a id="eth">Ethylene</a></span>
              <div class="part-box">
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                        </td>
                          <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065000">BBa_K1065000</a></td>
                          <td class="name">EFE</td>
@@ Line 37: / Line 59: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                        </td>
                          <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065002">BBa_K1065002</a></td>
                          <td class="name">EFE with double-terminator</td>
@@ Line 52: / Line 78: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                        </td>
                          <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065001">BBa_K1065001</a></td>
                          <td class="name">EFE arabinose-regulated</td>
@@ Line 69: / Line 99: @@
              <div id="label2" class="label">
              </div>
-             <span class="title">Methyl salicylate</span>
+             <span class="title"><a id="mesa">Methyl salicylate</a></span>
              <div class="part-box">
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                        </td>
                          <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065100">BBa_K1065100</a></td>
                          <td class="name">BSMT1</td>
@@ Line 80: / Line 113: @@
                      <tr>
                          <td colspan="2" class="descr">
-                             This part encodes the BSMT1, it is the enzyme that methylates salycilic acid to obtain  methyl salycilate. It’s the original part  from the Eau de coli MIT iGEM 2006 project, resubmitted by us in pSB1C3.
+                             This part encodes BSMT1, an enzyme that methylates salycilic acid to obtain methyl salycilate. It is the original part from the '<i>Eau de coli</i>' MIT iGEM 2006 project, re-submitted (<i>and resurrected</i>) by us in pSB1C3.
                          </td>
                      </tr>
@@ Line 89: / Line 122: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                        </td>
                          <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065103">BBa_K1065103</a></td>
                          <td class="name">PchBA</td>
@@ Line 95: / Line 131: @@
                      <tr>
                          <td colspan="2" class="descr">
-                             PchA ,the enzyme catalyzing salicylate biosynthesis isochorismate, and PchB that is the isochorismate-pyruvate lyase for that form the precursor for the salycilic acid.
+                             PchA is the enzyme responsible for the formation of isochorismate from chorismate. The next step is performed by the PchB enzyme, which converts isochorismate in salicylic acid. This is another part from the '<i>Eau de coli</i>' MIT iGEM 2006 project that we re-submitted (<i>and resurrected</i>) in pSB1C3.
                          </td>
                      </tr>
@@ Line 104: / Line 140: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                        </td>
                          <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065104">BBa_K1065104</a></td>
                          <td class="name">SAM synthetase</td>
@@ Line 110: / Line 149: @@
                      <tr>
                          <td colspan="2" class="descr">
-                             This part encodes the SAM (S-AdoMet) synthetase gene, (which we friendly called "uncle SAM") . It is a methyl donor required to produce MeSA and other methylated molecules.
+                             This part encodes the SAM (S-AdoMet) synthetase gene, which we friendly call "uncle SAM". SAM is a methyl donor required to produce MeSA and other methylated molecules.
                          </td>
                      </tr>
@@ Line 119: / Line 158: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                        </td>
                          <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065101">BBa_K1065101</a></td>
                          <td class="name">BSMT1 arabinose-regulated</td>
@@ Line 125: / Line 167: @@
                      <tr>
                          <td colspan="2" class="descr">
-                             A methyltransferase (BSMT1) regulated by the araCpBAD inducible promoter.
+                             A methyltransferase (BSMT1) regulated by the araCpBAD  promoter.
                          </td>
                      </tr>
@@ Line 134: / Line 176: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
-                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065105">Bba_K1065105</a></td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
-                         <td class="name">SAM synthetase IPTG-regulated</td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                        </td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065105">BBa_K1065105</a></td>
+                         <td class="name">SAM synthetase under pLac promoter</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.
+                             This is SAM synthetase (<i>uncle SAM</i>) regulated by the constitutive Lac promoter.
                          </td>
                      </tr>
@@ Line 149: / Line 194: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                        </td>
                          <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065102">BBa_K1065102</a></td>
-                         <td class="name">PchBA IPTG-regulated and BSMT1 arabinose-regulated</td>
+                         <td class="name">PchBA under pLac and BSMT1 arabinose-regulated</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.
+                             The wintergreen device: PchBA under a Lac promoter followed by BSMT1 under the araCpBAD promoter.
                          </td>
                      </tr>
@@ Line 164: / Line 212: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                        </td>
                          <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065106">BBa_K1065106</a></td>
                          <td class="name">BSMT1, PchBA and SAM synthetase</td>
@@ Line 170: / Line 221: @@
                      <tr>
                          <td colspan="2" class="descr">
-                             An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production.
+                             An improved version of the wintergreen device: BBa_K1065102 with SAM synthetase to boost production.
                          </td>
                      </tr>
@@ Line 181: / Line 232: @@
              <div id="label3" class="label">
              </div>
-             <span class="title">Blue light</span>
+             <span class="title"><a id="blue">Blue light</a></span>
              <div class="part-box">
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
-                         <td class="code">Bba_K1065301</td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
-                         <td class="name">amilCP IPTG-regulated</td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                        </td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065302">BBa_K1065302</a></td>
+                         <td class="name">Blue light circuit to produce amilGFP</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             We attached pLac promoter to amilCP gene. This part does not work (sadly we saw no blue color). In fact, we started from BBa_K592009 which lacks the RBS (it is a coding part). <i>No RBS, no party!</i>
+                             A device to characterize our improved part BBa_K1065305 controlled by pLac promoter.
                          </td>
                      </tr>
@@ Line 201: / Line 256: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
-                         <td class="code">Bba_K1065300</td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
-                         <td class="name">BBa_K952003 with Yf1 and FixJ IPTG-regulated.</td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                        </td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065305">BBa_K1065305</a></td>
+                         <td class="name">BBa_K952003 improved</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             pLac+Yf1-FixJ-pFixK2-amil GFP without RBS and with pLac upstream.
+                             No rbs, no party!!!!We improved BBa_K952003 by mutagenesis to add the missing RBS, now the part works.
                          </td>
                      </tr>
@@ Line 216: / Line 275: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
-                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065303">BBa_K1065303</a></td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
-                         <td class="name">Yf1+FixJ+pFixK2</td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                        </td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065309">BBa_K1065309</a></td>
+                         <td class="name">Blue light ethylene producing device</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             The starting point to build a blue-light sensing circuit: the photosensor, the response regulator with its promoter. This part was build merging BBa_K592016 and BBa_K592006.
+                             Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE.
                          </td>
                      </tr>
@@ Line 231: / Line 293: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                        </td>
                          <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065310">BBa_K1065310</a></td>
-                         <td class="name">Yf1+FixJ+pFixK2+Inverter+pLambda+amilCP</td>
+                         <td class="name">Complete blue-light sensing circuit with an inverter</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             This is a complete blue-light sensing circuit, inverted with cI. Yf1 and FixJ are under the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced.
+                             Yf1 and FixJ are regulated by the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced.
                          </td>
                      </tr>
@@ Line 246: / Line 312: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
-                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065302">BBa_K1065302</a></td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
-                         <td class="name">BBa_K1065300 improved</td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
+                        </td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065311">BBa_K1065311</a></td>
+                         <td class="name">Complete blue-light sensing device with inverter, amilCP and EFE</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             The same as BBa_K1065300: we performed a mutagenesis to add the missing RBS, now this works!!! It is: pLac+Yf1+FixJ+pFixK2+amilGFP.
+                             Our complete circuit composed by:J23100, Yf1 and FixJ, pFixK2, CI, pLambda, amilCP and EFE.
                          </td>
                      </tr>
@@ Line 261: / Line 330: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
-                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065305">BBa_K1065305</a></td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
-                         <td class="name">BBa_K952003 improved</td>
+                        </td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065306">BBa_K1065306</a></td>
+                         <td class="name">J23100+Yf1+FixJ</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             The same as BBa_K952003: we performed a mutagenesis to add the missing RBS, now this works!!! It is: Yf1+FixJ+pFixK2+amilGFP.
+                             Yf1 (photosensor) and FixJ (response regulator) under the Anderson promoter (BBa_J23100).
                          </td>
                      </tr>
@@ Line 276: / Line 347: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
-                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065306">BBa_K1065306</a></td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
-                         <td class="name">J23100+Yf1+FixJ</td>
+                        </td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065307">BBa_K1065307</a></td>
+                         <td class="name">J23100+Yf1+FixJ (with terminators)</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             Yf1 and FixJ under the Anderson promoter (BBa_J23100).
+                             The same as BBa_K1065306, just with the terminators at the end. This part was not submitted to the registry. If you are interested please contact us.
                          </td>
                      </tr>
@@ Line 291: / Line 364: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
-                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065204">BBa_K1065204</a></td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
-                         <td class="name">pXyl+EFE</td>
+                        </td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065308">BBa_K1065308</a></td>
+                         <td class="name">BBa_K1065307 with inverter</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             EFE in pXyl vector, specific for B. subtilis.
+                             This is an intermediate part to build a blue light inducible genetic circuit with the inverter. This part is in pSB1A2 and it was not submitted to the registry. If you are interested in this part please contact us.
                          </td>
                      </tr>
@@ Line 306: / Line 381: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
-                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065309">BBa_K1065309</a></td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
-                         <td class="name">BBa_K1065302+BBa_K1065002</td>
+                        </td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065303">BBa_K1065303</a></td>
+                         <td class="name">Yf1+FixJ+pFixK2</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE.
+                             The starting point to build a blue-light sensing circuit: the photosensor (Yf1) and  the response regulator (FixJ) with its promoter (pFixK2). This part was build merging BBa_K592016 and BBa_K592006.
                          </td>
                      </tr>
                  </table>
              </div>
-        </div>
-        <!-- Bacillus -->
-        <div class="box">
-            <div id="label4" class="label">
-            </div>
-            <span class="title">Bacillus</span>
              <div class="part-box">
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
-                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065201">BBa_K1065201</a></td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/1e/Tn-2013-parts-Banana-green-parts.png" />
-                         <td class="name">pXyl+GFP</td>
+                        </td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065300">BBa_K1065300</a></td>
+                         <td class="name">Blue light circuit to produce amilGFP</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production.
+                             It does not work beacuse it lacks the RBS before the amilGFP gene.
                          </td>
                      </tr>
                  </table>
              </div>
+        </div>
+        <!-- Bacillus -->
+        <div class="box">
+            <div id="label4" class="label">
+            </div>
+            <span class="title"><a id="bacillus"><i>Bacillus subtilis</i></a></span>
              <div class="part-box">
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
-                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065106">BBa_K1065106</a></td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/1e/Tn-2013-parts-Banana-green-parts.png" />
-                         <td class="name">BSMT1, PchBA and SAM synthetase</td>
+                        </td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065201">BBa_K1065201</a></td>
+                         <td class="name">pXyl+GFP</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840).
+                             A <i>B. subtilis</i> xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840).
                          </td>
                      </tr>
@@ Line 358: / Line 439: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
-                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065204">BBa_K1065204</a></td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/1e/Tn-2013-parts-Banana-green-parts.png" />
-                         <td class="name">pXyl+EFE</td>
+                        </td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065203">BBa_K1065203</a></td>
+                         <td class="name">Efe+Bba_B0015 in pSpac.</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             EFE in pXyl vector, specific for B. subtilis.
+                             EFE in pSpac vector, specific for <i>B. subtilis</i>.
                          </td>
                      </tr>
@@ Line 373: / Line 456: @@
                  <table>
                      <tr>
-                         <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp; <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />&nbsp;</td>
+                         <td rowspan="2" class="grade">
-                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065311">BBa_K1065311</a></td>
+                            <img src="https://static.igem.org/mediawiki/2013/1/1e/Tn-2013-parts-Banana-green-parts.png" />
-                         <td class="name">Complete blue-light sensing device with inverter, amilCP and EFE</td>
+                        </td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065204">BBa_K1065204</a></td>
+                         <td class="name">pXyl+EFE</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             Our complete circuit composed by:J23100, Yf1 and FixJ, pFixK2, CI, pLambda, amilCP and EFE.
+                             EFE in pXyl vector, specific for <i>B. subtilis</i>.
                          </td>
                      </tr>
@@ Line 386: / Line 471: @@
          </div>
+    </div>
+</div>
+<div class="container">
+    <div class="sheet-2">
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