Latest revision as of 18:12, 28 October 2013

Project Overview

Aromatic pollutants are becoming a worldwide concern. Monitoring aromatics in the environment, however, remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed a comprehensive biosensors toolkit to detect aromatics in the environment.

Transcriptional regulators sensing each general class of aromatic compounds were first bioinformatically determined using the genomic data from prokaryotes, and then utilized to build biosensor circuits in living cells. Genetic tailoring such as promoter engineering was performed to tune their properties functionally. Most of these novel biosensors proved to be capable of faithfully sensing a specific group of aromatics. Furthermore, the synergistic/antagonistic effects between their inducers was carefully examined; this allowed the combination of these biosensors to profile aromatics for the ease of practical applications.

In order to expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were gleaned from natural metabolic pathways, working as Adaptors to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits. Additionally, for the ease of practical analysis, we have constructed a genetic device called the "Band-pass Filter" to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter are able to robustly quantify the aromatics in environmental samples.

In conclusion, Peking iGEM has significantly enriched the repertoire of biosensors for aromatic compounds. These novel biosensors, together with the Adaptors and the Band-pass Filter, will serve as intriguing synthetic biological tools for diverse practical applications, including pollution monitoring, bioremediation, and metabolic process control.

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 		<li id="PKU_navbar_Home" class="Navbar_Item">
-			<a href="https://2013.igem.org/Team:Peking">home</a>
+			<a href="https://2013.igem.org/Team:Peking">Home</a>
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 			</ul>
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+                                <div class="BackgroundofSublist"></div>
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 		<li id="PKU_navbar_Project" class="Navbar_Item">
-			<a href="https://2013.igem.org/Team:Peking/Project">project</a>
+			<a href="https://2013.igem.org/Team:Peking/Project">Project</a>
 			<ul id="Project_Sublist">
-                                 <li><a href="https://2013.igem.org/Team:Peking/Project/AutoSensorMining">Auto Sensor Mining</a></li>
+                                <div class="BackgroundofSublist"></div>
+                                 <li><a href="https://2013.igem.org/Team:Peking/Project/SensorMining">Biosensor Mining</a></li>
 				<li><a href="https://2013.igem.org/Team:Peking/Project/BioSensors">Biosensors</a></li>
-				<li><a href="https://2013.igem.org/Team:Peking/Project/Plugins">Plug-ins</a></li>
+				<li><a href="https://2013.igem.org/Team:Peking/Project/Plugins">Adaptors</a></li>
 				<li><a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">Band-pass Filter</a></li>
-                                 <li><a href="https://2013.igem.org/Team:Peking/Project/Application">Application</a></li>
+                                 <li><a href="https://2013.igem.org/Team:Peking/Project/Devices">Devices</a></li>
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 		</li>
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-			<a href="https://2013.igem.org/Team:Peking/Model">model</a>
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+                                <div class="BackgroundofSublist"></div>
+                                <li><a href="https://2013.igem.org/Team:Peking/Model">Band-pass Filter</a></li>
+                                <li><a href="https://2013.igem.org/Team:Peking/ModelforFinetuning">Biosensor Fine-tuning</a></li>
+			</ul>
+		</li>
+                        <li id="PKU_navbar_HumanPractice" class="Navbar_Item" style="width:90px">
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-			<a href="https://2013.igem.org/Team:Peking/Safety">safety</a>
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 			<ul id="HumanPractice_Sublist">
-                                 <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/Questionnaire">Questionnaire</a></li>
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-				<li><a href="https://2013.igem.org/Team:Peking/HumanPractice/FactoryVisit">Factory Visit</a></li>
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-                                 <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/iGEMWorkshop">iGEM Workshop</a></li>
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-				<li><a href="https://2013.igem.org/Team:Peking/HumanPractice/ModeliGEM">Model iGEM</a></li>
+                                 <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/ModeliGEM">Practical Analysis</a></li>
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             <ul id="ProjectList">
-                 <li><a href="https://2013.igem.org/Team:Peking/Project/AutoSensorMining">Auto sensor mining</a><li>
+                 <li class="SensorsListItem"><a href="https://2013.igem.org/Team:Peking/Project/SensorMining">Biosensor Mining</a><li>
-                 <li><a href="https://2013.igem.org/Team:Peking/Project/BioSensors">Biosensors</a><li>
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+                 <li class="SensorsListItem"><a href="https://2013.igem.org/Team:Peking/Project/Plugins">Adaptors</a><li>
-                 <li><a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">Band-pass Filter</a><li>
+                 <li class="SensorsListItem"><a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">Band-pass Filter</a><li>
-                 <li><a href="https://2013.igem.org/Team:Peking/Project/Application">Applications</a><li>
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                  </ul>
       </div>
       <div id="ProjectOverviewContainer">
-            <img id="ProjectOverviewBackground" src="https://static.igem.org/mediawiki/2013/2/20/Peking_Project_Overview.jpg" />
+            <img id="ProjectOverviewBackground" src="https://static.igem.org/mediawiki/igem.org/e/ed/Peking2013_Project_title.jpg" />
-            <h1 id="ProjectOverviewTitle">Project</h1>
+            <h1 id="ProjectOverviewTitle">Project Overview</h1>
-            <p id="ProjectOverviewContent">There are numerous classes of prokaryotes lives in the aromatics-rich environment. Part of them possess aromatic degradation pathways, and also transcriptional factors to monitor the environmental aromatics, and control degradation pathway to balance the degradation capacity. Peking iGEM team mainly focused on the various transcriptional factors, which can respond to various aromatic inducers. We characterized these biosensors and improve their performance by using well-characterized biobricks. We expanded the detection range by coupling enzymes in upper pathways and biosensors to degrade aromatics to detectable compounds. For quantitative detection, we construct the band pass filter to detect a certain range of concentration. The appliance of microfluidics may construct a concentration gradient, and a certain concentration’s response can reflect the original concentration of environmental samples. With the application of this device, we can achieve easy and quantitative detection of environmental aromatics, and at the same time, remarkably enrich the library of inducible biosensors.
-</p>
       </div>
+     <p id="ProjectOverviewContent">
+Aromatic pollutants are becoming a worldwide concern. Monitoring aromatics in the environment, however, remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed <b>a comprehensive biosensors toolkit</b> to detect aromatics in the environment.
+</br></br>
+Transcriptional regulators sensing each general class of aromatic compounds were first <a href="https://2013.igem.org/Team:Peking/Project/SensorMining"><b>bioinformatically determined</b></a> using the genomic data from prokaryotes, and then utilized to build <a href="https://2013.igem.org/Team:Peking/Project/BioSensors"><b>biosensor circuits</b></a> in living cells. Genetic tailoring such as promoter engineering was performed to tune their properties functionally. Most of these novel biosensors proved to be capable of faithfully sensing a specific group of aromatics. Furthermore, the <a href="https://2013.igem.org/Team:Peking/Project/BioSensors/MulticomponentAnalysis">synergistic/antagonistic effects</a> between their inducers was carefully examined; this allowed the combination of these biosensors to profile aromatics for the ease of practical applications.
+</br></br>
+In order to expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were gleaned from natural metabolic pathways, working as <a href="https://2013.igem.org/Team:Peking/Project/Plugins"><b>Adaptors</b></a> to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits. Additionally, for the ease of practical analysis, we have constructed a genetic device called the "<a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter"><b>Band-pass Filter</b></a>" to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter are able to robustly quantify the aromatics in environmental samples.
+</br></br>
+In conclusion, Peking iGEM has <b>significantly enriched the repertoire of biosensors for aromatic compounds</b>. These novel biosensors, together with the Adaptors and the Band-pass Filter, will serve as intriguing synthetic biological tools for diverse practical applications, including pollution monitoring, bioremediation, and metabolic process control.
+</p>
 </div><!--end of major body-->