Team:Peking

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<a href="https://2013.igem.org/Team:Peking">home</a>
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<a href="https://2013.igem.org/Team:Peking">Home</a>
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<a href="https://2013.igem.org/Team:Peking/Project">Project</a>
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                                 <li><a href="https://2013.igem.org/Team:Peking/Project/AutoSensorMining">Auto Sensor Mining</a></li>
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                                 <li><a href="https://2013.igem.org/Team:Peking/Project/SensorMining">Biosensor Mining</a></li>
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                                 <li><a href="https://2013.igem.org/Team:Peking/Project/Devices">Devices</a></li>
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                                <li><a href="https://2013.igem.org/Team:Peking/ModelforFinetuning">Biosensor Fine-tuning</a></li>
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                                <li><a href="https://2013.igem.org/Team:Peking/DataPage/Parts">Parts</a></li>
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<li><a href="https://2013.igem.org/Team:Peking/DataPage/JudgingCriteria">Judging Criteria</a></li>
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                                 <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/Questionnaire">Questionnaire</a></li>
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                                 <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/ModeliGEM">Practical Analysis</a></li>
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<li><a href="https://2013.igem.org/Team:Peking/HumanPractice/iGEMWorkshop">Team Communication</a></li>
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<div id="ProjectTitle">
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<h1 id="ProjectName">Aromatics Scouts</h1>
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<h1 id="ProjectName">Aromatics Scouts<img id="LittleScout" src="https://static.igem.org/mediawiki/igem.org/8/8a/Peking2013_home_Telescope.png" /></h1>
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                 <h1 id="ProjectSubname">A fast, easy and accurate method to detect toxic aromatic compounds</h1>
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                 <h1 id="ProjectSubname">A Comprehensive Biosensor Toolkit for Profiling Aromatic Compounds in the Environment</h1>
   
   
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<p id="PreLocation">
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<B>★Presentation:</B> Room 34-101, Saturday 12:30 PM, Session 2
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      &ensp;&ensp;&ensp;&ensp;<B>★Poster:</B> Sunday, #13 (Stata)
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         <img id="MajorBodyBackground" src="https://static.igem.org/mediawiki/igem.org/9/97/Peking2013_Home_background.jpg" />
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            <p id="AbstractContent">Aromatic pollution is becoming a worldwide concern. Monitoring aromatics pollution, however, remains a substantial challenge. Noting the abundant genomic data of prokaryotes from aromatics-rich environment, Peking iGEM applied part mining to the genetic repertoire to develop a comprehensive set of transcriptional-regulator-based biosensors for aromatics. The transcription regulators for each typical class of organic compounds were first bioinformatically determined and then promoter engineering and protein engineering were performed to tune their properties functionally. To expand the detection profiles of biosensors, enzymes in upper pathways, working as plug-ins, were coupled with existing biosensors to degrade aromatics to detectable compounds. All these sensors are capable of detect a group of aromatics, and multi-sensor assay may provide an insight of detect certain components in samples.  
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              <p id="AbstractContent">Monitoring aromatic compounds in the environment remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed a functionally comprehensive biosensor toolkit to profile aromatics in the environment.  
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Additionally, for the ease of detection, we have constructed a band-pass filter to detect a specific range of concentration. Responses of biosensors equipped with band-pass filter can robustly reflect the concentration of environmental samples.  
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Transcriptional regulators that each detect a specific class of aromatics were first bioinformatically determined; and then utilized to build a comprehensive set of biosensor circuits. Characterization on the detection profiles of individual biosensors and the orthogonality/crosstalk between them proved that these biosensors are very capable at profiling aromatics present in water.  
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In conclusion, Peking iGEM has remarkably enriched the library of biosensors for aromatics and enabled quantitative in situ detection for environmental monitoring. These biosensors as well-characterized synthetic biological tools, we expect, will be also intriguing for metabolic engineering, such as metabolic process control.</p>
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Moreover, for the ease of practical applications, two types of genetic devices were also developed as plug-ins for biosensors: "Adaptors", a set of conceptually novel devices to convert undetectable compounds into detectable compounds, and "Band-pass Filter", a "concentration filter" that allows the detection of analyte concentration within a specific range.  
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We expect that these novel biosensors, together with the plug-in devices, will serve as intriguing synthetic biological tools for diverse practical applications.
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               <h1 id="Appendix1Title">We have a date in the database</h1>
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               <h1 id="Appendix1Title"><i>Mining aromatics-sensing Biobricks <br/>from the genomic database</i></h1>
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                     <h1 class="AppendixHeadLine">Auto Sensor Mining</h1>
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                     <h1 class="AppendixHeadLine">Biosensor Mining</h1>
                     <img id="Appendix1Icon" class="AppendixIcon" src="https://static.igem.org/mediawiki/igem.org/1/13/Peking2013_home_appendix1icon.png" />
                     <img id="Appendix1Icon" class="AppendixIcon" src="https://static.igem.org/mediawiki/igem.org/1/13/Peking2013_home_appendix1icon.png" />
 +
                    <p>The core component of our biosensor toolkit is the transcriptional regulators that sense aromatics. For the comprehensiveness of aromatics-sensing, a data-mining process was conducted to mine transcriptional regulators for each typical class of aromatics from the database Uniprot. </p>
 +
                    <a href="https://2013.igem.org/Team:Peking/Project/SensorMining">LEARN MORE</a>
               </div>
               </div>
         </div>
         </div>
         <div id="AppendixBox2" class="SmallBoxes">
         <div id="AppendixBox2" class="SmallBoxes">
-
               <h1 id="Appendix2Title">Light of glory shall shed<br/>upon those who see</h1>
+
               <h1 id="Appendix2Title"><i>High-performance, profile-<br/>specific biosensors</i> </h1>
               <div id="Appendix2Cover" >
               <div id="Appendix2Cover" >
                     <img id="AppendixImg2" src="https://static.igem.org/mediawiki/igem.org/1/17/Peking2013_home_appendix2.jpg" />
                     <img id="AppendixImg2" src="https://static.igem.org/mediawiki/igem.org/1/17/Peking2013_home_appendix2.jpg" />
                     <h1 class="AppendixHeadLine">Biosensors</h1>
                     <h1 class="AppendixHeadLine">Biosensors</h1>
                     <img id="Appendix2Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/b/b2/Peking2013_home_appendix2icon.png" />
                     <img id="Appendix2Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/b/b2/Peking2013_home_appendix2icon.png" />
 +
                    <p>A comprehensive set of biosensor circuits have been implemented using the aromatics-sensing transcriptional regulators. Each biosensor has a specific aromatics-sensing profile. Furthermore, the orthogonality of their sensing profiles was carefully assessed for practical applications.</p>
 +
                    <a href="https://2013.igem.org/Team:Peking/Project/BioSensors">LEARN MORE</a>
               </div>
               </div>
         </div>
         </div>
         <div id="AppendixBox3" class="SmallBoxes">
         <div id="AppendixBox3" class="SmallBoxes">
-
               <h1 id="Appendix3Title">Be thou me, impetuous one!</h1>
+
               <h1 id="Appendix3Title"><i>Converting the undetectable into<br/>the detectable</i></h1>
               <div id="Appendix3Cover" >
               <div id="Appendix3Cover" >
                     <img id="AppendixImg3" src="https://static.igem.org/mediawiki/igem.org/1/1b/Peking2013_home_appendix3.jpg" />
                     <img id="AppendixImg3" src="https://static.igem.org/mediawiki/igem.org/1/1b/Peking2013_home_appendix3.jpg" />
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                     <h1 class="AppendixHeadLine">Plug-ins</h1>
+
                     <h1 class="AppendixHeadLine">Adaptors</h1>
                     <img id="Appendix3Icon"  class="AppendixIcon" src="https://static.igem.org/mediawiki/igem.org/c/cd/Peking2013_home_appendix3icon.png" />
                     <img id="Appendix3Icon"  class="AppendixIcon" src="https://static.igem.org/mediawiki/igem.org/c/cd/Peking2013_home_appendix3icon.png" />
 +
                    <p> To expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were taken from natural metabolic pathways, working as Adaptors to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits.</p>
 +
                    <a href="https://2013.igem.org/Team:Peking/Project/Plugins">LEARN MORE</a>
               </div>
               </div>
         </div>
         </div>
         <div id="AppendixBox4" class="SmallBoxes">
         <div id="AppendixBox4" class="SmallBoxes">
-
               <h1 id="Appendix4Title">My heart beats only for the special you</h1>
+
               <h1 id="Appendix4Title"><i>Rapidly determining <br/> the analyte concentration</i></h1>
               <div id="Appendix4Cover" >
               <div id="Appendix4Cover" >
                     <img id="AppendixImg4" src="https://static.igem.org/mediawiki/igem.org/e/e6/Peking2013_home_appendix4.jpg" />
                     <img id="AppendixImg4" src="https://static.igem.org/mediawiki/igem.org/e/e6/Peking2013_home_appendix4.jpg" />
                     <h1 class="AppendixHeadLine">Band-pass Filter</h1>
                     <h1 class="AppendixHeadLine">Band-pass Filter</h1>
                     <img id="Appendix4Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/4/42/Peking2013_home_appendix4icon.png" />
                     <img id="Appendix4Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/4/42/Peking2013_home_appendix4icon.png" />
 +
                    <p>For the ease of practical analysis, a genetic device called "Band-pass Filter" has been constructed to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter can robustly quantify the aromatics in environmental samples. </p>
 +
                    <a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">LEARN MORE</a>
               </div>
               </div>
         </div>
         </div>
         <div id="AppendixBox5" class="SmallBoxes">
         <div id="AppendixBox5" class="SmallBoxes">
-
               <h1 id="Appendix5Title">I leave no trace of wings in the air,<br/> but I'm glad I had my flight.</h1>
+
               <h1 id="Appendix5Title"><i>Communications, Questionnaire survey,  <br/>Factory Visit</i> and interview</h1>
               <div id="Appendix5Cover" >
               <div id="Appendix5Cover" >
                     <img id="AppendixImg5" src="https://static.igem.org/mediawiki/igem.org/d/da/Peking2013_home_appendix5.jpg" />
                     <img id="AppendixImg5" src="https://static.igem.org/mediawiki/igem.org/d/da/Peking2013_home_appendix5.jpg" />
                     <h1 class="AppendixHeadLine">Human Practice</h1>
                     <h1 class="AppendixHeadLine">Human Practice</h1>
                     <img id="Appendix5Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/8/85/Peking2013_home_appendix5icon.png" />
                     <img id="Appendix5Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/8/85/Peking2013_home_appendix5icon.png" />
 +
                    <p>To obtain the information about public awareness and the situation of aromatics pollution, a survey including factory visit and questionnaires has been conducted. We also guided an iGEM HS team and held "Model iGEM" as a competition without competitiveness.</p>
 +
                    <a href="https://2013.igem.org/Team:Peking/HumanPractice">LEARN MORE</a>
               </div>
               </div>
         </div>         
         </div>         

Latest revision as of 18:10, 28 October 2013

Aromatics Scouts

A Comprehensive Biosensor Toolkit for Profiling Aromatic Compounds in the Environment

★Presentation: Room 34-101, Saturday 12:30 PM, Session 2     ★Poster: Sunday, #13 (Stata)

Monitoring aromatic compounds in the environment remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed a functionally comprehensive biosensor toolkit to profile aromatics in the environment.

Transcriptional regulators that each detect a specific class of aromatics were first bioinformatically determined; and then utilized to build a comprehensive set of biosensor circuits. Characterization on the detection profiles of individual biosensors and the orthogonality/crosstalk between them proved that these biosensors are very capable at profiling aromatics present in water.

Moreover, for the ease of practical applications, two types of genetic devices were also developed as plug-ins for biosensors: "Adaptors", a set of conceptually novel devices to convert undetectable compounds into detectable compounds, and "Band-pass Filter", a "concentration filter" that allows the detection of analyte concentration within a specific range.

We expect that these novel biosensors, together with the plug-in devices, will serve as intriguing synthetic biological tools for diverse practical applications.

Mining aromatics-sensing Biobricks
from the genomic database

Biosensor Mining

The core component of our biosensor toolkit is the transcriptional regulators that sense aromatics. For the comprehensiveness of aromatics-sensing, a data-mining process was conducted to mine transcriptional regulators for each typical class of aromatics from the database Uniprot.

LEARN MORE

High-performance, profile-
specific biosensors

Biosensors

A comprehensive set of biosensor circuits have been implemented using the aromatics-sensing transcriptional regulators. Each biosensor has a specific aromatics-sensing profile. Furthermore, the orthogonality of their sensing profiles was carefully assessed for practical applications.

LEARN MORE

Converting the undetectable into
the detectable

Adaptors

To expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were taken from natural metabolic pathways, working as Adaptors to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits.

LEARN MORE

Rapidly determining
the analyte concentration

Band-pass Filter

For the ease of practical analysis, a genetic device called "Band-pass Filter" has been constructed to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter can robustly quantify the aromatics in environmental samples.

LEARN MORE

Communications, Questionnaire survey,
Factory Visit and interview

Human Practice

To obtain the information about public awareness and the situation of aromatics pollution, a survey including factory visit and questionnaires has been conducted. We also guided an iGEM HS team and held "Model iGEM" as a competition without competitiveness.

LEARN MORE

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