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Team:BYU Provo/Notebook/Phage Purification/Springexp/Period4/Dailylog
From 2013.igem.org
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| + | : <u> '''Phage Purification''' </u> </font> | ||
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]] | : [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]] | ||
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| - | <html> | + | <html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Dailylog"><< Previous</a> |
<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period5/Dailylog" style="display:block;float:right;">Next >></a> | <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period5/Dailylog" style="display:block;float:right;">Next >></a> | ||
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- Set up CsCl gradient | - Set up CsCl gradient | ||
[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.17CsClGradient|6.17 CsCl Gradient]] | [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.17CsClGradient|6.17 CsCl Gradient]] | ||
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| + | - Prepared T4 and T7 cultures. | ||
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| + | :[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period4/Exp/6.17PhagePreparation|6.17 Phage Preparation]] | ||
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<font size="4"> '''6/19/13''' </font> | <font size="4"> '''6/19/13''' </font> | ||
| - | - Began first step of purification process using phage from [[Team:BYU_Provo/Notebook/Phage_Purification/ | + | - Began first step of purification process using phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period4/Exp/6.17PhagePreparation|6.17 Phage Preparation]]. |
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| + | : [[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period1/Exp/6.19PEGPurification|6.19 PEG Purification]] | ||
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| + | - Ran spot tests on collected band from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.17CsClGradient|6.17 CsCl Gradient]]. | ||
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| + | :[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period4/Exp/6.19Spot Test|6.19 Spot Test]] | ||
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| + | <font size="4"> '''6/20/13''' </font> | ||
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| + | - Finished [[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period1/Exp/6.19PEGPurification|6.19 PEG Purification]] by centrifuging solution at 10,000 g 15 minutes at 4<sup>◦</sup> C, removing supernatant, and suspending the pellet in phage suspension buffer. | ||
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| + | <font size="4"> '''6/21/13''' </font> | ||
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| + | We centrifuged the pellet at 10,000 g for 15 min at 4<sup>◦</sup> C after suspending it to clean up the walls of the tube. | ||
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Latest revision as of 00:04, 28 September 2013
| Phage Purification May - June Notebook: June 10 - June 23 Daily Log
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6/10/13 - Prepared RNase A for future use. - Prepared T4 and T7 cultures.
6/12/13 - Began first step of purification process using phage from 6.10 Phage Preparation.
6/14/13 - Worked on updating the website.
6/17/13 - Set up CsCl gradient 6.17 CsCl Gradient - Prepared T4 and T7 cultures.
6/19/13 - Began first step of purification process using phage from 6.17 Phage Preparation. - Ran spot tests on collected band from 6.17 CsCl Gradient.
6/20/13 - Finished 6.19 PEG Purification by centrifuging solution at 10,000 g 15 minutes at 4◦ C, removing supernatant, and suspending the pellet in phage suspension buffer.
6/21/13 We centrifuged the pellet at 10,000 g for 15 min at 4◦ C after suspending it to clean up the walls of the tube.
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