Team:Tokyo Tech/More/Attribution and Contribution

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<p style="line-height:0em; text-indent:0em;" name="top">Attribution and Contribution</p>
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<h1>Attribution</h1>
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<h1>Attribution
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We are proud to say that all of the ideas we worked on this summer were proposed by undergraduate members of our own team.  We also want to thank all those advisors and instructors who helped us discuss these ideas and guided us through doing the experiments.  The whole experimental data produced by the Tokyo Tech 2013 team comes from experiments carried out all by undergraduates.
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[[Image:Titech2013-attribution_contribution_Fig_1_everyone.jpg|400px|thumb|right|We are team Tokyo Tech!]]
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We are proud to say that all of the ideas we worked on this summer were proposed by undergraduate members of our own team.   
 +
We also want to thank all those advisors and instructors who helped us discuss these ideas and guided us through doing the experiments.  The whole experimental data produced by team Tokyo Tech 2013 comes from experiments carried out all by undergraduates.
</p><p>
</p><p>
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The idea of "Crosstalk circumvention" "M13 phage" and "Plant hormone" were all proposed by undergraduate members of our team (Ema Hatanaka, Tatsuhiro Isozaki and Ryota Fuziwara).  Modeling related to "Crosstalk circumvention" and "Plant hormone" was also done by undergraduate students (Yochiku Shi).
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The ideas of "Crosstalk circumvention" "M13 phage" and "Farming" were all proposed by undergraduate members of our team (Emma Hatanaka, Tatsuhiro Isozaki and Ryota Fujiwara).  Modeling related to "Crosstalk circumvention" and "Farming" were also done by undergraduate students (Yochiku Shi).
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</h2>
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<h3>--Student team members--
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</h3>
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<h2>
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<h3><p>Our undergraduate members carried out all experiments!</p></h3>
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[[Image:Titech2013_attribution_experiment_%E5%93%81%E8%B3%AA1.jpg|170px|thumb|left|Our lab notes!!!]]
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<p style="text-indent:0em;"><i>performing all cloning (transformatin, culture cells, miniprep)</i>:<br></p>
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<div style="text-align:right;"> mainly Ken, Hiroshi, Tatsuya, Shunta, Sairi and Shinya with helping of other students</div>
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<p style="text-indent:0em;"><i>performing plaque assay (assay for confirmation of the parts concerning M13 phage)</i>:<br></p>
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<div style="text-align:right;"> Naoki and Tatsuhiro </div>
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<p style="text-indent:0em;"><i>performing bio assay (assay concerning plants)</i>:<br></p>
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<div style="text-align:right;"> Ryota</div>
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<p style="text-indent:0em;"><i>performing fluorescence assay (experiment using flow cytometer)</i>:<br></p>
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<div style="text-align:right;"> Yuhta, Emma, Sara, Ryota and Hengyu</div>
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<p style="text-indent:0em;"><i>performing parts construction (restriction digest, ligation)</i>:<br></p>
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<div style="text-align:right;"> Ken, Hiroshi, Tatsuya, Shunta, Sairi and Shinya</div>
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<p style="text-indent:0em;"><i>modeling  (simulation of the genetic circuit )</i>:<br></p>
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<div style="text-align:right;"> Yochiku and Masanori</div>
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<p style="text-indent:0em;"><i>performing identification of cytokinins (UPLC)</i>:<br></p>
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<div style="text-align:right;"> Shunta </div>
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</h2>
</h2>
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<h1>Contribution</h1>
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<h1>Contribution
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<h3>--General support--</h3>
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</h1>
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<h3>--General support--
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</h3>
<h2>
<h2>
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Prof. Kiga was letting us use his lab for some experiments.  He also led us to get dazzling results in iGEM competition too.
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Prof. Kiga was letting us use his lab for some our experiments.  He also led us to get dazzling results in iGEM competition too.
<br>Prof. Yamamura and Prof. Konagaya supported us in many ways to give us a better researching life.
<br>Prof. Yamamura and Prof. Konagaya supported us in many ways to give us a better researching life.
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<br>Prof. Ayukawa gave us experimental environment that we could be satisfied.
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<br>Prof. Ayukawa gave us an experimental environment that we could be satisfied.
<br>Mr. Sekine and Mr. Amikura gave us the helpful advice for playing an active part in iGEM competition.
<br>Mr. Sekine and Mr. Amikura gave us the helpful advice for playing an active part in iGEM competition.
<br>Promega provided us the part of restriction enzyme we used at no charge.
<br>Promega provided us the part of restriction enzyme we used at no charge.
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<br>National Institute of Genetics provided us MG1655.
<br>National Institute of Genetics provided us MG1655.
</h2>
</h2>
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<h3>--Chemotaxis support--</h3>
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<h3>--Instrumental analysis support--
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</h3>
<h2>
<h2>
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Biomaterial Analysis Center, Technical Department, sequenced the sequence of the plasmids which we had asked.
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Biomaterial Analysis Center, Technical Department, sequenced the sequence of plasmids which we had asked.
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<br>Mr. Yoshihisa Sei is working in Tokyo Institute of Technology Center for Advanced Materials Analysis. He gave us a technical guidance of UPLC and provided the equipment.
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<br>Mr. Sei is working in Tokyo Institute of Technology Center for Advanced Materials Analysis. He gave us a technical guidance of [https://2013.igem.org/Team:Tokyo_Tech/Experiment/Quantitative_Analysis_of_Cytokinin#2._Identification_of_cytokinin_by_ultra-performance_liquid_chromatography_.28UPLC.29 UPLC] and provided the equipment.
</h2>
</h2>
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<h3>--Plant hormone support--</h3>
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<h3>--Plant hormone support--
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</h3>
<h2>
<h2>
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Integrated DNA Technologies was synthesized gene, AtIPT4 and AtIPT7 which we had asked.
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Integrated DNA Technologies was synthesized gene, [https://2013.igem.org/Team:Tokyo_Tech/Experiment/Quantitative_Analysis_of_Cytokinin#2._Identification_of_cytokinin_by_ultra-performance_liquid_chromatography_.28UPLC.29 <i>AtIPT4</i>] and [https://2013.igem.org/Team:Tokyo_Tech/Experiment/Quantitative_Analysis_of_Cytokinin#2._Identification_of_cytokinin_by_ultra-performance_liquid_chromatography_.28UPLC.29 <i>AtIPT7</i>] which we had asked.
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<br>Wako Pure Chemical Industries, Ltd. sold us cytokinins (iP and tZ) and Auxin (IAA).
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<br>Wako Pure Chemical Industries, Ltd. sold us [https://2013.igem.org/Team:Tokyo_Tech/Experiment/Quantitative_Analysis_of_Cytokinin#1._Quantitative_analysis_for_cytokinin_using_cotyledons_of_cucumber cytokinins (iP and tZ)] and Auxin (IAA).
</h2>
</h2>
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<h3>--Cucumis sativus L. support--</h3>
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<h3>--[https://2013.igem.org/Team:Tokyo_Tech/Experiment/Quantitative_Analysis_of_Cytokinin#1._Quantitative_analysis_for_cytokinin_using_cotyledons_of_cucumber <i>Cucumis sativus L.</i>] support--
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</h3>
<h2>
<h2>
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Bio Creative Design Room of Tokyo Tech gave us equipment (pestle and mortar) and reagents (IAA, CK, acetone and DMSO).
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Bio Creative Design Room of Tokyo Tech gave us equipment (pestles and mortars) and reagents (IAA, CK, acetone and DMSO).
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<br>Prof. Ohta was letting us use his lab for some experiments.  He also gave us advice on bioassay too.
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<br>Prof. Ohta was letting us use his darkroom for some experiments.  He also gave us advice on bioassay too.
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<br>Collaboration Center for Design and Manufacturing of Tokyo Tech was letting us use the lab for our bioassay.
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<br>Collaboration Center for Design and Manufacturing of Tokyo Tech was letting us use its lab for our bioassay.
</h2>
</h2>
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<h3>--Human practices support--</h3>
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<h3>--Human Practice support--
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</h3>
<h2>
<h2>
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Mr. Shigeru Katayama who is working in Tokyo Institute of Technology Graduate School of Innovation Management gave us his opinion on issues of environmental and beneficiary survey of our projects.
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[https://2013.igem.org/Team:Tokyo_Tech/Human_Practice#6-2._Thinking_about_ethical_problem_on_our_project Mr. Katayama] who is working in Tokyo Institute of Technology Graduate School of Innovation Management gave us his opinion on issues of environmental and beneficiary survey of our projects.
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<br>Mr. Tomio Kawabata who is working in JA ZENNOH CHIBA advised what problems and benefits will be occurred if our project was applied.
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<br>[https://2013.igem.org/Team:Tokyo_Tech/Human_Practice#8._Human_Practice_4:_THE_TAKEDA_YOUNG_ENTREPRENEURSHIP_AWARD Mr. Kawabata] who is working in JA ZENNOH CHIBA advised what problems and benefits will be occurred if our project was applied.
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<br>Takeda Science Foundation introduced us in their book.  
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<br>[https://2013.igem.org/Team:Tokyo_Tech/Human_Practice#7._Human_Practice_3:_Interviewed_by_The_Takeda_Foundation. Takeda Science Foundation] introduced us in their book.  
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<br>Mr. Yukio Sato (a teacher of Shinjuku high school) was proposed the experiment classroom, and he led us.
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<br>Mr. Sato (a teacher of Shinjuku high school) proposed [https://2013.igem.org/Team:Tokyo_Tech/Human_Practice#2._Human_Practice_1:_Experiment_Workshop the experimental classroom], and led us.
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<br>Ms. Naoko Inoue (a teacher of Sagamihara high school) and Ms. Kanako Miyamoto (a teacher of Toin Gakuen) collected a lot of high school students to our experiment classroom.
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<br>Ms. Inoue (a teacher of Sagamihara high school) and Ms. Miyamoto (a teacher of Toin Gakuen) collected lots of high school students to [https://2013.igem.org/Team:Tokyo_Tech/Human_Practice#2._Human_Practice_1:_Experiment_Workshop our experimental classroom].
</h2>
</h2>
<h3>--And last but not least--</h3>
<h3>--And last but not least--</h3>
<h2>
<h2>
We would like to thank all the people who came to our progress presentations and gave useful feedback and input:<br>
We would like to thank all the people who came to our progress presentations and gave useful feedback and input:<br>
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Fumio Arisaka, Toshiaki Fukui, Takeshi Hata, Junji Hirota, Hiroshi Iwasaki, Satoshi Nakamura, Shunichiro Ogura, Hideki Taguchi, Masahiro Takinoue, Yasunori Tanji, Hirokazu Urabe, Masaaki Wachi, Robert F. Whittier, Taku Nakamura, Goshi Sugano, Naoki Tsukuda and Nobuaki Yasuo.
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Fumio Arisaka, Toshiaki Fukui, Takeshi Hata, Junji Hirota, Hiroshi Iwasaki, Satoshi Nakamura, Shunichiro Ogura, Hideki Taguchi, Masahiro Takinoue, Yasunori Tanji, Hirokazu Urabe, Masaaki Wachi, Robert F. Whittier, Daniel Berrar, Mai Miura, Taku Nakayama, Goshi Sugano, Naoki Tsukuda and Nobuaki Yasuo.
</h2>
</h2>
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</div><br>
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Latest revision as of 17:13, 28 October 2013


Attribution and Contribution

Attribution

We are team Tokyo Tech!

We are proud to say that all of the ideas we worked on this summer were proposed by undergraduate members of our own team. We also want to thank all those advisors and instructors who helped us discuss these ideas and guided us through doing the experiments. The whole experimental data produced by team Tokyo Tech 2013 comes from experiments carried out all by undergraduates.

The ideas of "Crosstalk circumvention" "M13 phage" and "Farming" were all proposed by undergraduate members of our team (Emma Hatanaka, Tatsuhiro Isozaki and Ryota Fujiwara). Modeling related to "Crosstalk circumvention" and "Farming" were also done by undergraduate students (Yochiku Shi).

--Student team members--

Our undergraduate members carried out all experiments!

Our lab notes!!!

performing all cloning (transformatin, culture cells, miniprep):

mainly Ken, Hiroshi, Tatsuya, Shunta, Sairi and Shinya with helping of other students

performing plaque assay (assay for confirmation of the parts concerning M13 phage):

Naoki and Tatsuhiro

performing bio assay (assay concerning plants):

Ryota

performing fluorescence assay (experiment using flow cytometer):

Yuhta, Emma, Sara, Ryota and Hengyu

performing parts construction (restriction digest, ligation):

Ken, Hiroshi, Tatsuya, Shunta, Sairi and Shinya

modeling (simulation of the genetic circuit ):

Yochiku and Masanori

performing identification of cytokinins (UPLC):

Shunta



Contribution

--General support--

Prof. Kiga was letting us use his lab for some our experiments. He also led us to get dazzling results in iGEM competition too.
Prof. Yamamura and Prof. Konagaya supported us in many ways to give us a better researching life.
Prof. Ayukawa gave us an experimental environment that we could be satisfied.
Mr. Sekine and Mr. Amikura gave us the helpful advice for playing an active part in iGEM competition.
Promega provided us the part of restriction enzyme we used at no charge.
Mr. J. Collins provided us JM2.300.
National Institute of Genetics provided us MG1655.

--Instrumental analysis support--

Biomaterial Analysis Center, Technical Department, sequenced the sequence of plasmids which we had asked.
Mr. Sei is working in Tokyo Institute of Technology Center for Advanced Materials Analysis. He gave us a technical guidance of UPLC and provided the equipment.

--Plant hormone support--

Integrated DNA Technologies was synthesized gene, AtIPT4 and AtIPT7 which we had asked.
Wako Pure Chemical Industries, Ltd. sold us cytokinins (iP and tZ) and Auxin (IAA).

--Cucumis sativus L. support--

Bio Creative Design Room of Tokyo Tech gave us equipment (pestles and mortars) and reagents (IAA, CK, acetone and DMSO).
Prof. Ohta was letting us use his darkroom for some experiments. He also gave us advice on bioassay too.
Collaboration Center for Design and Manufacturing of Tokyo Tech was letting us use its lab for our bioassay.

--Human Practice support--

Mr. Katayama who is working in Tokyo Institute of Technology Graduate School of Innovation Management gave us his opinion on issues of environmental and beneficiary survey of our projects.
Mr. Kawabata who is working in JA ZENNOH CHIBA advised what problems and benefits will be occurred if our project was applied.
Takeda Science Foundation introduced us in their book.
Mr. Sato (a teacher of Shinjuku high school) proposed the experimental classroom, and led us.
Ms. Inoue (a teacher of Sagamihara high school) and Ms. Miyamoto (a teacher of Toin Gakuen) collected lots of high school students to our experimental classroom.

--And last but not least--

We would like to thank all the people who came to our progress presentations and gave useful feedback and input:
Fumio Arisaka, Toshiaki Fukui, Takeshi Hata, Junji Hirota, Hiroshi Iwasaki, Satoshi Nakamura, Shunichiro Ogura, Hideki Taguchi, Masahiro Takinoue, Yasunori Tanji, Hirokazu Urabe, Masaaki Wachi, Robert F. Whittier, Daniel Berrar, Mai Miura, Taku Nakayama, Goshi Sugano, Naoki Tsukuda and Nobuaki Yasuo.


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