Team:SDU-Denmark/Tour42

From 2013.igem.org

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<h2>The Process</h2>
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<h2>Helping others</h2>
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<h4>From thought to rubber</h4>
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<h4>Together, we are stronger</h4>
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<p class='intro'>
 
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“Ones more into the breach, dear friends, once more” - Henry V
 
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</p>
 
<p>
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We used an arsenal of self-written Standard Operating Procedures (SOPs) to execute the vast amount of laboratory work. Each was written in collaboration with a person with experience in the field of the procedure and then approved by a second competent person. As such, it was strictly forbidden to change the SOPs without consulting the rest of the team, and any changes were then implemented in agreement. It was hereby secured that everyone in the lab were following the same procedures each time.  
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<b>Collaborations</b><br>
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<a class="popupImg alignRight" style="width:500px" target="_blank" href="https://static.igem.org/mediawiki/2013/f/f9/SDU2013_Helping_others_1.JPG" title="Visit from DTUs and KUs iGEM teams">
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  <img src="https://static.igem.org/mediawiki/2013/0/0f/SDU2013_Small_Helping_others_1.JPG" style="width:500px"/>
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Nothing prepares a team better for a jamboree than hand-shoe-football!
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</a>
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A large part of the iGEM competition’s ideal is to help other teams; teams this year and in years to come. The point of the Biobrick system is through means of cooperation to make biological engineering simple, and to emphasize the importance of collaboration. One of the gold medal requirements is to help another team, either by modelling or through lab work.
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</p><p>
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As part of our project, we helped the Edinburgh Team with a computer modulation, made a video to help the team from the University of Copenhagen start a new iGEM educational system, and hosted a Jamboree preparing meetup for the danish iGEM teams.
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<p>
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<b>List of the different SOPs;</b> Each name is a hyperlink connected to an online PDF of the SOP.
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iGEM2013_SOP0001_v01_MM_ON_culture_of_E.coli<br>
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<b>Modelling for Edinburgh</b><br>
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iGEM2013_SOP0002_v01_TK_Table_autoclave<br>
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iGEM2013_SOP0003_v01_MM_Bacterial_freezing_stock<br>
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Basically, there are two ways to optimize a system of microbacterial organisms. At SDU, we optimized the internal pathways, ensuring better production from each cell. Alternatively, optimization of the cells’ external environment (with the intention to maximize the number of cells) will increase production speed. This is the solution that Edinburgh’s team worked on.
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iGEM2013_SOP0004_v01_Primer_Design<br>
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iGEM2013_SOP0005_v01_USER primer design<br>
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</p><p>
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iGEM2013_SOP0006_v01_PCR protocol for USER cloning<br>
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iGEM2013_SOP0007_v01_excision and ligation of PCR product in USER cloning<br>
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Our collaboration has been mutually beneficial: We have gained an understanding of the second type of optimization with the option of future implementation into our work, while being able to help Edinburgh with inevitable problems, which arose when simulating systems.
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iGEM2013_SOP0008_v01_preparing primers<br>
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iGEM2013_SOP0009_v01_TSB transformation<br>
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</p><p>
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iGEM2013_SOP0010_v01_Phusion PCR<br>
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iGEM2013_SOP0011_v01_PCR with MyTaq<br>
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Specifically, we helped the Edinburgh team to identify their system as being stiff, which requires different techniques to solve, for example better stability properties of the integrators. Additionally, the Edinburgh team required help to solve the nonlinear equations that arose from the model.
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iGEM2013_SOP0012_v01_Restriction_Digest<br>
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iGEM2013_SOP0013_v01_Nano-drop<br>
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iGEM2013_SOP0014_v01_Gel purification<br>
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iGEM2013_SOP0015_v01_Ligation<br>
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iGEM2013_SOP0016_v01_Making LB and LA media<br>
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iGEM2013_SOP0017_v01_Fast_digest<br>
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iGEM2013_SOP0018_v01_Agar plates with Cm<br>
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iGEM2013_SOP0019_v01_Plasmid Miniprep<br>
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iGEM2013_SOP0020_v01_MM_Agarose_gel_DNA<br>
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iGEM2013_SOP0021_v01_TK_Colony_PCR_with_MyTaq<br>
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iGEM2013_SOP0022_v01_Speedy Vac<br>
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iGEM2013_SOP0023_v01_SDM_ PCR reactions for Site directed mutagenesis of Dxs from B. subtilis<br>
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iGEM2013_SOP0024_v01_Growth_experiment<br>
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iGEM2013_SOP0025_v01_Growth for RNA purification - IPTG inducable plasmid<br>
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iGEM2013_SOP0026_v01_Growth for RNA purification - ara inducible plasmid<br>
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iGEM2013_SOP0027_v01_RNA purification<br>
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iGEM2013_SOP0028_v01_Northen blotting<br>
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iGEM2013_SOP0029_v01_Kinating probe for northern blot<br>
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</p>
</p>
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<p>
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<a href="https://2013.igem.org/Team:Edinburgh/Collaboration" title="" target="_blank">Edinburgh's description of our collaboration.</a> A further description of our collaboration can be seen under Modelling.
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</p>
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<p>
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<b>Danish iGEM meetup</b><br>
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<a class="popupImg alignRight" style="width:300px" target="_blank" href="https://static.igem.org/mediawiki/2013/a/a5/SDU2013_Helping_others_2.JPG" title="">
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  <img src="https://static.igem.org/mediawiki/2013/a/af/SDU2013_Small_Helping_others_2.JPG" style="width:300px" />
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Enjoying a beer in the sun, discussing ideas and the upcoming jamboree.
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</a>
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The past couple of years the Scandinavian iGEM teams have met  over a weekend to discuss projects, experiences, and presentations. This year, it was our turn to host the event. Unfortunately, only Danish teams had a chance to participate, as the chosen weekend coincided with the start of the new semester.  The first day of the meetup featured team building exercises and (in the spirit of great Danish tradition) a beer to get the talk going amongst the teams. The following morning, we presented our projects and preliminary posters, got feedback for improvements in preparation of the Jamboree, and discussed lab-related topics during a workshop. And importantly, we enjoyed each other’s company and exquisitely nerdy humor.
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</p><p>
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<b>Bricks of Knowledge:</b><br>
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During the meetup, we heard about the University of Copenhagen’s Bricks of Knowledge-project and decided to support the great initiative. We made a short movie about what we have learned in terms of keeping order in lab to conserve an overview of the project. We believe we did this quite well, and our little tips were included in the video. This video can be seen on the <a href="https://2013.igem.org/Team:UNIK_Copenhagen/BricksOfKnowledgeMovieBricks" target="_blank">website of the 2013 University of Copenhagen iGEM team.</a>
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Latest revision as of 10:49, 28 October 2013

Helping others

Together, we are stronger

Collaborations
Nothing prepares a team better for a jamboree than hand-shoe-football! A large part of the iGEM competition’s ideal is to help other teams; teams this year and in years to come. The point of the Biobrick system is through means of cooperation to make biological engineering simple, and to emphasize the importance of collaboration. One of the gold medal requirements is to help another team, either by modelling or through lab work.

As part of our project, we helped the Edinburgh Team with a computer modulation, made a video to help the team from the University of Copenhagen start a new iGEM educational system, and hosted a Jamboree preparing meetup for the danish iGEM teams.

Modelling for Edinburgh
Basically, there are two ways to optimize a system of microbacterial organisms. At SDU, we optimized the internal pathways, ensuring better production from each cell. Alternatively, optimization of the cells’ external environment (with the intention to maximize the number of cells) will increase production speed. This is the solution that Edinburgh’s team worked on.

Our collaboration has been mutually beneficial: We have gained an understanding of the second type of optimization with the option of future implementation into our work, while being able to help Edinburgh with inevitable problems, which arose when simulating systems.

Specifically, we helped the Edinburgh team to identify their system as being stiff, which requires different techniques to solve, for example better stability properties of the integrators. Additionally, the Edinburgh team required help to solve the nonlinear equations that arose from the model.

Edinburgh's description of our collaboration. A further description of our collaboration can be seen under Modelling.

Danish iGEM meetup
Enjoying a beer in the sun, discussing ideas and the upcoming jamboree. The past couple of years the Scandinavian iGEM teams have met over a weekend to discuss projects, experiences, and presentations. This year, it was our turn to host the event. Unfortunately, only Danish teams had a chance to participate, as the chosen weekend coincided with the start of the new semester. The first day of the meetup featured team building exercises and (in the spirit of great Danish tradition) a beer to get the talk going amongst the teams. The following morning, we presented our projects and preliminary posters, got feedback for improvements in preparation of the Jamboree, and discussed lab-related topics during a workshop. And importantly, we enjoyed each other’s company and exquisitely nerdy humor.

Bricks of Knowledge:
During the meetup, we heard about the University of Copenhagen’s Bricks of Knowledge-project and decided to support the great initiative. We made a short movie about what we have learned in terms of keeping order in lab to conserve an overview of the project. We believe we did this quite well, and our little tips were included in the video. This video can be seen on the website of the 2013 University of Copenhagen iGEM team.