Team:KIT-Kyoto/Notebook/ATF2

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Latest revision as of 02:26, 28 September 2013

ATF2

10th August

We performed PCR to amplify the ATF2 gene.

F:3' CGATCCCATGGGGATGGAAGATATAGAAGGATACGAACCACA

R:5' CGATCGGATCCAAAAGCGACGCAAATTCGCCGATGGTTTG

Buffer	50µL
dNTP	20µL
Primer mix	1µL
DNA sample	0.5µL
KOD-FX	2µL
H₂O	26.5µL
Total	100µL

PCR products were electrophoresed in 1% agarose gel.

We detected a DNA band at around 1600bp.

PCR reactions were carried out (total volume was 300µL).

PCR products were electrophoresed in 1% agarose gel.

The PCR product with the size (1.6 kb) was verified as ATF2.

The PCR products of ATF2 was purified by ethanol precipitation

12th August

The PCR product of ATF2 were digested with the XhoI.

ATF2	5µL
Buff	2µL
H₂O	12µL
Xho1	1µL

No DNA was detected.

We have repeated the PCR reactions to amplify ATF2 under the same conditions as those carried out on 10th, August.

14th August

1.

PCR reaction was carried out by using the following primer for pSB1C3.

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGATCACTAGTATTAAAGCGACGCAAATTCGCCGATGGTTTG

PCR products were electrophoresed in 1% agarose gel.

The PCR product with the size (1.6 kb) was verified as ATF2.

The PCR products of ATF2 was purified by ethanol precipitation.

2.

The ATF2 gene and pET15b were purified by ethanol precipitation.

<i>ATF2</i> was digested with the XhoI for verification.

ATF2	5µL
Buff	2µL
H₂O	12µL
Xho1	1µL

PCR products were electrophoresed in 1% agarose gel.

The PCR products with the expected size were detected.

ATF2 treated ethanol precipitation and pET-15b was digested with the NcoI and BamHI.

pET-15b	87µL
Nco1	1µL
BamH1	1µL
Buff.	10µL
BSA	1µL
(total 100µL)

ATF2	24µL
Nco1	1µL
BamH1	1µL
Buff.	3µL
BSA	1µL
(total 30µL)

It was electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

17th August

pET-15b was digested with the Nco1 and BamH1.

pET-15b	87µL
Nco1	1µL
BamH1	1µL
Buff.	10µL
BSA	1µL
(total 100µL)

We treated the vector with 1µL BAP for 30 minutes.

It was electrophoresed in 1% Blue gel

ATF2 were extracted from the Blue gel

19th August

The ATF2 gene and pET-15b were purified.

We ligated pET-15b and ATF2.

ATF2	5µL
pET-15b	5µL
L.MIX	10µL
total 20µL

After the ligatation, we have transformed them into E.coli cells.

20th August

We got approximately 70 colonies and cultured them furthermore.

21th August

We checked the insertion of DNA by colony cracking.

We could see correct bands of plasmid DNA only from five samples.

We cultured each transformant in LB liquid medium containing ampicillin.

PCR reaction was carried out using the following primers

1-1

F:3' TCGGCAATGCACAGTCATGGCCATAGTAGA

R:5' TTATTCAATTGGATGAGAAATCACCTTTATGAAATCATGCT

1-2

F:3' CATCTTGAATTCGATGACTTGATTATGAATAATCAACCA

R:5' CGATCACTAGTATTAAAGCGACGCAAATTCGCCGATGGTTTG

2-1

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

2-2

F:3' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

R:5' ATCATCTTCGTAATCCTTGATGTGAATAGTGC

2.

PCR products were electrophoresed in 1% agarose gel.

The PCR products with the size (1.6 kb) were verified as ATF2.

The PCR products of ATF2 were purified by ethanol precipitation and digested with the following composition.

1-1

ATF2	25µL
Mfe1	2µL
Buff.	3µL
Total 30µL

1-2

ATF2	25µL
EcoR1	2µL
Buff.	3µL
Total 30µL

2-1

ATF2	25µL
Nsi1	2µL
Buff.	3µL
Total 30µL

2-2

ATF2	25µL
Pst1	2µL
Buff.	3µL
Total 30µL

It were electrophoresed in 1% Blue gel

ATF2 were extracted from the Blue gel

22th August

I

1

ATF2 gene extracted from the Blue gel was treated ethanol precipitation.

2

Ligation of 2-1 and 2-2 was performed by using Ligation mix.

1-1 with 1-2 and 2-1 with 2-2

The PCR products of ATF2 gene was purified.

It was electrophoresed in 1% agarose gel.

No DNA was detected.

II

1

PCR reaction was carried out using the primers.

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

1-1

ATF2	25µL
Mfe1	2µL
Buff.	3µL
Total 30µL

1-2

ATF2	25µL
EcoR1	2µL
Buff.	3µL
Total 30µL

2-1

ATF2	25µL
Nsi1	2µL
Buff.	3µL
Total 30µL

2-2

ATF2	25µL
Pst1	2µL
Buff.	3µL
Total 30µL

It was electrophoresed in 1% Blue gel.

Any DNA fragment was not detected.

III

PCR reaction was carried out using the following primers.

1-1

F:3' TCGGCAATGCACAGTCATGGCCATAGTAGA

R:5' TTATTCAATTGGATGAGAAATCACCTTTATGAAATCATGCT

1-2

F:3' CATCTTGAATTCGATGACTTGATTATGAATAATCAACCA

R:5'

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

1-1

ATF2	25µL
Mfe1	2µL
Buff.	3µL
Total 30µL

1-2

ATF2	25µL
EcoR1	2µL
Buff.	3µL
Total 30µL

It were electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

ATF2 extracted from the Blue gel was purified.

IV

PCR reaction was carried out using the primer.

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

1-1

ATF2	25µL
Mfe1	2µL
Buff.	3µL
Total 30µL

1-2

ATF2	25µL
EcoR1	2µL
Buff.	3µL
Total 30µL

23th August

The plasmids (d, e, f, I, o) were digested with Nco1 and BamH1.

We applied them to 1% agarose gel electrophoresis and checked them.

Sample d, e, f, i had two bands and we cultured them.

The plasmids (d, e, f, I,) were digested with BsiWI and checked.

We detected the correct size band.

We minipreped and transformed them into BL21(DE3).

I

Sample 1.

ATF2 gene (Ⅳ) extracted from the Blue gel was purified by ethanol precipitation.

Sample 2.

ATF2 gene (III) extracted from the Blue gel was purified by ethanol precipitation.

Ligation of sample 1 and 2 was performed by Ligation mix.

PCR reaction was carried out using the primers

PCR products were electrophoresed in 1% agarose gel, and several fragments were detected.

II

PCR reaction was carried out using the primers

1-1

F:3' TCGGCAATGCACAGTCATGGCCATAGTAGA

R:5' TTATTCAATTGGATGAGAAATCACCTTTATGAAATCATGCT

1-2

F:3' CATCTTGAATTCGATGACTTGATTATGAATAATCAACCA

R:5' CGATCACTAGTATTAAAGCGACGCAAATTCGCCGATGGTTTG

The PCR products of ATF2 gene were purified, and digested with Mfe and EcoRI.

After the digestion, the ATF2 gene was electrophoresed in 1% Blue gel.

The ATF2 genes were extracted from the Blue gel and purified.

Ligation of 2-1 and 2-2 was performed using Ligation mix.

PCR reaction was carried out using the primers

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGATCACTAGTATTAAAGCGACGCAAATTCGCCGATGGTTTG

24th August

I

PCR reaction was carried out using the following primers.

2-1

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

2-2

F:3' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

R:5' ATCATCTTCGTAATCCTTGATGTGAATAGTGC

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

2-1

ATF2	25µL
Nsi1	2µL
Buff.	3µL
Total 30µL

2-2

ATF2	25µL
Pst1	2µL
Buff.	3µL
Total 30µL

It was electrophoresed in 1% Blue gel.

No DNA was detected.

II

１

PCR react ion was carried out using the primer.

The PCR products for ATF2 was treated ethanol precipitation.

It was digested with the following composition.

2-1

ATF2	25µL
Nsi1	2µL
Buff.	3µL
Total 30µL

2-2

ATF2	25µL
Pst1	2µL
Buff.	3µL
Total 30µL

It were electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

ATF2 extracted from the Blue gel was purified.

Ligation of 2-1 and 2-2 was performed using Ligation mix.

III

1.

PCR reaction was carried out using the primer.

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

ATF2	25µL
EcoR1	2µL
Buff.	3µL
Total 30µL

It was electrophoresed in 1% agarose gel.

Multiple DNA fragments were detected.

IV

１

PCR reaction was carried out using the primers.

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

ATF2	25µL
EcoR1	2µL
Buff.	3µL
Total 30µL

It was electrophoresed in 1% agarose gel.

Multiple DNA fragments were detected for the DIAP2.

26th August

Cell free extract was prepared and applied to SDS-PAGE to detect ATF2 protein.

27th August

PCR products were electrophoresed in 1% agarose gel.

The PCR products were treated ethanol precipitation.

They were digested.

They were electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

ATF2 extracted from the Blue gel was treated ethanol precipitation.

Ligation of 2-1 and 2-2 was performed using Ligation mix.

We purified them.

28th August

The PCR products was treated ethanol precipitation.

PCR reaction was carried out by using the primer.

PCR products were electrophoresed in 1% agarose gel.

No DNA was detected.

We performed PCR, purified, and digested again.

We ligated and purified them.

29th August

I

PCR reaction was carried out using the primer.

PCR products were electrophoresed in 1% agarose gel.

No DNA band was detected.

II

PCR reaction was carried out using the following primers.

2-1

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

2-2

F:3' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

R:5' ATCATCTTCGTAATCCTTGATGTGAATAGTGC

The PCR products were treated ethanol precipitation.

It was digested with the following composition.

2-1

ATF2	25µL
Nsi1	2µL
Buff.	3µL
Total 30µL

2-2

ATF2	25µL
Pst1	2µL
Buff.	3µL
Total 30µL

It was electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

ATF2 extracted from the Blue gel was purified.

We ligated them and purified it.

We performed PCR to amplify it.

Cell free extract was prepared with FastBreak Cell Lysis Reagent and applied to SDS-PAGE.

30th August

I

1

PCR reaction was carried out using the following primers

1-1

F:3' TCGGCAATGCACAGTCATGGCCATAGTAGA

R:5' TTATTCAATTGGATGAGAAATCACCTTTATGAAATCATGCT

1-2

F:3' CATCTTGAATTCGATGACTTGATTATGAATAATCAACCA

R:5'

The PCR products were purfied.

It was digested with the following composition.

2-1

ATF2	25µL
Nsi1	2µL
Buff.	3µL
Total 30µL

2-2

ATF2	25µL
Pst1	2µL
Buff.	3µL
Total 30µL

It were electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

ATF2 extracted from the Blue gel was purified.

We ligated them and purified it.

PCR reaction was carried out using the following primers for pSB1C3.

PCR products were electrophoresed in 1% agarose gel.

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

ATF2	25µL
Pst1	2µL
Buff.	3µL
Total 30µL

PCR products were electrophoresed in 1% agarose gel.

The PCR products of ATF2 gene was purified.

II

１

We performed PCR (prepared 8/29).

PCR reaction was carried out using the following primers.

2-1

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

2-2

F:3' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

R:5' ATCATCTTCGTAATCCTTGATGTGAATAGTGC

The PCR products were purified.

It was digested with the following composition.

2-1

ATF2	25µL
Nsi1	2µL
Buff.	3µL
Total 30µL

2-2

ATF2	25µL
Pst1	2µL
Buff.	3µL
Total 30µL

It was electrophoresed in 1% Blue gel.

ATF2 was extracted from the Blue gel.

ATF2 extracted from the Blue gel was purified.

We ligated it.

We purified it and performed PCR.

2^nd September

We purified the mutant of ATF2.

We digested it and cc3 with Xba1 and Spe1.

We electrophoresed and extracted them in and from 1% Blue gel.

We purified them.

We ligated and transformed them into E.coli cells.

4^th September

We purified the mutant of ATF2.

Three tubes of pSB1C3 were treated ethanol precipitation

We add 25mL H₂O and digested it with Xba1 and Spe1.

We electrophoresed and extracted them in and from 1% Blue gel.

We purified them.

We ligated and transformed them into E.coli cells.

5^th September

We did rapid check of the insert by colony cracking (ATF2+pSB1C3).

But we detected no band.

We cultured them for 5 hours and minipreped.

We checked them by electrophoresing but couldn’t see any bands.

So, we thought them failed.

We cultured other sample (ATF2+pSB1C3).

@@ Line 13: / Line 13: @@
 <div id="doc-right" class="metro">
-<h2>ATF2</h2>
+<h2>ATF2</h2>
-<div class=WordSection1 style='layout-grid:18.0pt'>
 <p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>10th August</span></b></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR
-to amplify the <span class=MsoSubtleEmphasis><span style='color:black'>ATF2</span></span><span
+to amplify the <span class=MsoSubtleEmphasis><span style='color:black'><i>ATF2</i></span></span><i><span
-style='color:black'> g</span>ene.</span></p>
+style='color:black'> </span></i><span style='color:black'>g</span>ene.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>F:3'
@@ Line 41: / Line 38: @@
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:9.7pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>50uL</span></p>
+   style='font-size:11.0pt'>50&micro;L</span></p>
    </td>
   </tr>
@@ Line 54: / Line 51: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.7pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>20uL</span></p>
+   style='font-size:11.0pt'>20&micro;L</span></p>
    </td>
   </tr>
@@ Line 67: / Line 64: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.9pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>1uL</span></p>
+   style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 80: / Line 77: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.7pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>0.5uL</span></p>
+   style='font-size:11.0pt'>0.5&micro;L</span></p>
    </td>
   </tr>
@@ Line 93: / Line 90: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.7pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>2uL</span></p>
+   style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 100: / Line 97: @@
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:9.9pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>H2O</span></p>
+   style='font-size:11.0pt'>H<sub>2</sub>O</span></p>
    </td>
    <td width=66 valign=top style='width:49.8pt;border-top:none;border-left:none;
@@ Line 106: / Line 103: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.9pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>26.5uL</span></p>
+   style='font-size:11.0pt'>26.5&micro;L</span></p>
    </td>
   </tr>
@@ Line 119: / Line 116: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.7pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>100uL</span></p>
+   style='font-size:11.0pt'>100&micro;L</span></p>
    </td>
   </tr>
@@ Line 131: / Line 128: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reactions were
-carried out (total volume was 300μl).</span></p>
+carried out (total volume was 300&micro;L).</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR products were
@@ Line 138: / Line 135: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR product
 with the size (1.6 kb) was verified as <span class=MsoSubtleEmphasis><span
-style='color:windowtext'>ATF2.</span></span></span></p>
+style='color:windowtext'><i>ATF2</i>.</span></span></span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products of
-<span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2</span></span>
+<span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i></span></span>
 was purified by ethanol precipitation</span></p>
@@ Line 151: / Line 148: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR product of
-<span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2</span></span>
+<span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i></span></span>
 were digested with the <span class=MsoSubtleEmphasis><span style='color:windowtext'>Xho</span></span>I.</span></p>
@@ Line 160: / Line 157: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:17.1pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:windowtext'>ATF2</span></span></p>
+   style='font-size:11.0pt;color:windowtext'><i>ATF2</i></span></span></p>
    </td>
    <td width=61 valign=top style='width:46.1pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:17.1pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5μL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5&micro;L</span></p>
    </td>
   </tr>
@@ Line 175: / Line 172: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2μL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 181: / Line 178: @@
    <td width=64 valign=top style='width:47.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>H20</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>H<sub>2</sub>O</span></p>
    </td>
    <td width=61 valign=top style='width:46.1pt;border-top:none;border-left:none;
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>12μL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>12&micro;L</span></p>
    </td>
   </tr>
@@ Line 199: / Line 196: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1μL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 208: / Line 205: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We have repeated
 the PCR reactions to amplify <span class=MsoSubtleEmphasis><span
-style='color:windowtext'>ATF2</span></span> under the same conditions as those
+style='color:windowtext'><i>ATF2</i></span></span> under the same conditions as those
 carried out on 10th, August.</span></p>
@@ Line 222: / Line 219: @@
 <p class=MsoNormal align=left style='text-align:left'><b><span lang=EN-US
-style='font-size:14.0pt'>14th Augst</span></b></p>
+style='font-size:14.0pt'>14th August</span></b></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１．</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
-carried out by using the following primer for psb1c3.</span></p>
+carried out by using the following primer for pSB1C3.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT</span></p>
@@ Line 238: / Line 235: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR product
 with the size (1.6 kb) was verified as <span class=MsoSubtleEmphasis><span
-style='color:windowtext'>ATF2.</span></span></span></p>
+style='color:windowtext'><i>ATF2</i>.</span></span></span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2</span></span>
+of <span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i></span></span>
 was purified by ethanol precipitation.</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>２．</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The <i>ATF2 </i>gene
 and pET15b were purified by ethanol precipitation.</span></p>
-<p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>ATF2 </span></i><span
+<p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'><i>ATF2</i> </span></i><span
 lang=EN-US style='font-size:11.0pt'>was digested with the <span
 class=MsoSubtleEmphasis><span style='color:windowtext'>Xho</span></span>I for
@@ Line 264: / Line 261: @@
    <td width=61 valign=top style='width:46.1pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:17.1pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5μL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5&micro;L</span></p>
    </td>
   </tr>
@@ Line 275: / Line 272: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2μL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 281: / Line 278: @@
    <td width=64 valign=top style='width:47.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>H20</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>H<sub>2</sub>O</span></p>
    </td>
    <td width=61 valign=top style='width:46.1pt;border-top:none;border-left:none;
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>12μL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>12&micro;L</span></p>
    </td>
   </tr>
@@ Line 299: / Line 296: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1μL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 311: / Line 308: @@
 <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-style='font-size:11.0pt;color:black'>ATF2</span></span><span lang=EN-US
+style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span lang=EN-US
 style='font-size:11.0pt;color:black'> </span><span lang=EN-US style='font-size:
-.0pt'>treated ethanol precipitation and pet15b was digested with the <i>Nco</i>I
+.0pt'>treated ethanol precipitation and pET-15b was digested with the <i>Nco</i>I
 and <i>Bam</i>HI.</span></p>
@@ Line 325: / Line 322: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>87ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>87&micro;L</span></p>
    </td>
   </tr>
@@ Line 331: / Line 328: @@
    <td width=91 valign=top style='width:67.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nco1 </span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1 </span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 342: / Line 340: @@
    <td width=91 valign=top style='width:67.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BamH1 </span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bam</span></i><span
+  lang=EN-US style='font-size:11.0pt'>H1 </span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 358: / Line 357: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10&micro;L</span></p>
    </td>
   </tr>
@@ Line 369: / Line 368: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 375: / Line 374: @@
    <td width=147 colspan=2 valign=top style='width:110.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:19.55pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 100ul)</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 100&micro;L)</span></p>
    </td>
   </tr>
@@ Line 388: / Line 387: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:black'>ATF2</span></span><span lang=EN-US
+   style='font-size:11.0pt;color:black'><i>ATF2</i></span></span></p>
-  style='font-size:11.0pt;color:black'> </span><span lang=EN-US
-  style='font-size:11.0pt'>24ul</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24&micro;L</span></p>
    </td>
   </tr>
@@ Line 400: / Line 397: @@
    <td width=98 valign=top style='width:73.45pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nco1 </span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1 </span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 411: / Line 409: @@
    <td width=98 valign=top style='width:73.45pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BamH1 </span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bam</span></i><span
+  lang=EN-US style='font-size:11.0pt'>H1 </span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 427: / Line 426: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 438: / Line 437: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 444: / Line 443: @@
    <td width=154 colspan=2 valign=top style='width:115.7pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:19.55pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 30ul)</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 30&micro;L)</span></p>
    </td>
   </tr>
@@ Line 461: / Line 460: @@
 <p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>17th August</span></b></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PET15b was
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>pET-15b was
 digested with the <i>Nco</i>1 and <i>Bam</i>H1.</span></p>
@@ Line 473: / Line 472: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>87ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>87&micro;L</span></p>
    </td>
   </tr>
@@ Line 479: / Line 478: @@
    <td width=91 valign=top style='width:67.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nco1 </span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1 </span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 490: / Line 490: @@
    <td width=91 valign=top style='width:67.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BamH1 </span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bam</span></i><span
+  lang=EN-US style='font-size:11.0pt'>H1 </span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 506: / Line 507: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10&micro;L</span></p>
    </td>
   </tr>
@@ Line 517: / Line 518: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 523: / Line 524: @@
    <td width=147 colspan=2 valign=top style='width:110.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:19.55pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 100ul)</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 100&micro;L)</span></p>
    </td>
   </tr>
 </table>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We treated the DNA
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We treated the
-with 1ul BAP for 30 minutes.</span></p>
+vector with 1&micro;L BAP for 30 minutes.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>It was
@@ Line 548: / Line 549: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We ligated pET-15b
-with <i>ATF2</i>.</span></p>
+and <i>ATF2</i>.</span></p>
 <table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
@@ Line 561: / Line 562: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5&micro;L</span></p>
    </td>
   </tr>
@@ Line 572: / Line 573: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5&micro;L</span></p>
    </td>
   </tr>
@@ Line 583: / Line 584: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10&micro;L</span></p>
    </td>
   </tr>
@@ Line 589: / Line 590: @@
    <td width=147 colspan=2 valign=top style='width:110.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>total 20ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>total 20&micro;L</span></p>
    </td>
   </tr>
@@ Line 613: / Line 614: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&lt;<span
-class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2</span></span></span><span
+class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>+ pET15b&gt;</span></p>
-style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>＋</span><span lang=EN-US
-style='font-size:11.0pt'>pET15b&gt;</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We checked the
@@ Line 664: / Line 663: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>２</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR products were
@@ Line 674: / Line 673: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products of
-<span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2</span></span>
+<span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i></span></span>
-were purified by ethanol precipitation and digested with the following composition.</span></p>
+were purified by ethanol precipitation and digested with the following
+composition.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1-1</span></p>
@@ Line 690: / Line 690: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 696: / Line 696: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Mfe1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Mfe</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 712: / Line 713: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 718: / Line 719: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 733: / Line 734: @@
    padding:0mm 5.4pt 0mm 5.4pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:black'>ATF2</span></span><span
+   style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span
    style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif";color:black'>　</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 744: / Line 745: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>EcoR1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Eco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>R1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 760: / Line 762: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 766: / Line 768: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 787: / Line 789: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 793: / Line 795: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nsi1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nsi</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 809: / Line 812: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 815: / Line 818: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 835: / Line 838: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 841: / Line 844: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Pst1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 857: / Line 861: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 863: / Line 867: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 869: / Line 873: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>9</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>It were
@@ Line 886: / Line 888: @@
 <p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>22th August</span></b></p>
-<p class=MsoNormal><b><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅰ</span></b></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:11.0pt'>I</span></b></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span></p>
 <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-style='font-size:11.0pt;color:black'>ATF2</span></span><span lang=EN-US
+style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span lang=EN-US
 style='font-size:11.0pt;color:black'> </span><span lang=EN-US style='font-size:
 .0pt'>gene extracted from the Blue gel was treated ethanol precipitation.</span></p>
@@ Line 897: / Line 899: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>２</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Ligation of 2-1
@@ Line 906: / Line 908: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products of
-<span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>gene
+<span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i> </span></span>gene
 was purified.</span></p>
@@ Line 917: / Line 919: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅱ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>II</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 925: / Line 927: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>gene
+of <span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i> </span></span>gene
 was purified.</span></p>
@@ Line 944: / Line 946: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 950: / Line 952: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Mfe1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Mfe</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 966: / Line 969: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 972: / Line 975: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 992: / Line 995: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 998: / Line 1,001: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>EcoR1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Eco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>R1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,014: / Line 1,018: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,020: / Line 1,024: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,041: / Line 1,045: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,047: / Line 1,051: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nsi1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nsi</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,063: / Line 1,068: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,069: / Line 1,074: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,084: / Line 1,089: @@
    padding:0mm 5.4pt 0mm 5.4pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:black'>ATF2</span></span><span
+   style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span
    style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>　</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,095: / Line 1,100: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Pst1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,111: / Line 1,117: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,117: / Line 1,123: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,130: / Line 1,136: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅲ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>III</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 1,165: / Line 1,171: @@
    padding:0mm 5.4pt 0mm 5.4pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:black'>ATF2</span></span><span
+   style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span
    style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>　</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,176: / Line 1,182: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Mfe1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Mfe</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,192: / Line 1,199: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,198: / Line 1,205: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,218: / Line 1,225: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,224: / Line 1,231: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>EcoR1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Eco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>R1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,240: / Line 1,248: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,246: / Line 1,254: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,255: / Line 1,263: @@
 <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-style='font-size:11.0pt;color:black'>ATF2</span></span><span lang=EN-US
+style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span lang=EN-US
 style='font-size:11.0pt'> were extracted from the Blue gel.</span></p>
 <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-style='font-size:11.0pt;color:black'>ATF2</span></span><span lang=EN-US
+style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span lang=EN-US
 style='font-size:11.0pt;color:black'> </span><span lang=EN-US style='font-size:
 .0pt'>extracted from the Blue gel was purified.</span></p>
@@ Line 1,265: / Line 1,273: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅳ</span></p>
+<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>IV</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 1,271: / Line 1,279: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>gene
+of <span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i> </span></span>gene
 was purified.</span></p>
@@ Line 1,290: / Line 1,298: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,296: / Line 1,304: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Mfe1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Mfe</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,312: / Line 1,321: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,318: / Line 1,327: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,338: / Line 1,347: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,344: / Line 1,353: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>EcoR1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Eco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>R1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,360: / Line 1,370: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,366: / Line 1,376: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,378: / Line 1,388: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The plasmids (d,
-e, f, I, o) were digested with Nco1 and BamH1.</span></p>
+e, f, I, o) were digested with <i>Nco</i>1 and <i>Bam</i>H1.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We applied them to
@@ Line 1,401: / Line 1,411: @@
 <p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅰ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>I</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Sample 1.</span></p>
@@ Line 1,414: / Line 1,424: @@
 <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>ATF2</span></i><span
 lang=EN-US style='font-size:11.0pt'> gene (</span><span style='font-size:11.0pt;
-font-family:"ＭＳ 明朝","serif"'>Ⅲ</span><span lang=EN-US style='font-size:11.0pt'>)
+font-family:"ＭＳ 明朝","serif"'>III</span><span lang=EN-US style='font-size:11.0pt'>)
 extracted from the Blue gel was purified by ethanol precipitation.</span></p>
@@ Line 1,421: / Line 1,431: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
-carried out using the primers.</span></p>
+carried out using the primers </span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1-1 </span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>F:3'
-TCGGCAATGCACAGTCATGGCCATAGTAGA</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>R:5'
-TTATTCAATTGGATGAGAAATCACCTTTATGAAATCATGCT</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1-2 </span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>F:3'
-CATCTTGAATTCGATGACTTGATTATGAATAATCAACCA</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>R:5' CGATCACTAGTATTAAAGCGACGCAAATTCGCCGATGGTTTG</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR products were
@@ Line 1,445: / Line 1,438: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅱ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>II</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 1,464: / Line 1,457: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>R:5' CGATCACTAGTATTAAAGCGACGCAAATTCGCCGATGGTTTG</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <i>ATF2</i> gene were purified, and digested with Mfe and EcoR</span><span
+of <i>ATF2</i> gene were purified, and digested with <i>Mfe</i> and <i>Eco</i>R</span><span
-style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅰ</span><span lang=EN-US
+style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>I</span><span lang=EN-US
 style='font-size:11.0pt'>.</span></p>
@@ Line 1,478: / Line 1,469: @@
 were extracted from the Blue gel and purified.</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Ligation of 1-1
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Ligation of 2-1
-and 1-2 was performed using Ligation mix.</span></p>
+and 2-2 was performed using Ligation mix.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
-carried out using the primers</span></p>
+carried out using the primers </span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>R:5' CGATCACTAGTATTAAAGCGACGCAAATTCGCCGATGGTTTG</span></p>
 <p class=MsoNormal><b><span lang=EN-US>&nbsp;</span></b></p>
@@ Line 1,490: / Line 1,485: @@
 <p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>24th August</span></b></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅰ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>I</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 1,514: / Line 1,509: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>gene
+of <span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i> </span></span>gene
 was purified.</span></p>
@@ Line 1,533: / Line 1,528: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,539: / Line 1,534: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nsi1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nsi</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,555: / Line 1,551: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,561: / Line 1,557: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,576: / Line 1,572: @@
    padding:0mm 5.4pt 0mm 5.4pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:black'>ATF2</span></span><span
+   style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span
    style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif";color:black'>　</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,587: / Line 1,583: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Pst1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,603: / Line 1,600: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,609: / Line 1,606: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,622: / Line 1,619: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅱ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>II</span></p>
 <p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１</span></p>
@@ Line 1,628: / Line 1,625: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR react ion was
 carried out using the primer.</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2-1 </span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>R:5' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2-2</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>F:3'
-CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>R:5'
-ATCATCTTCGTAATCCTTGATGTGAATAGTGC</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
@@ Line 1,662: / Line 1,641: @@
    padding:0mm 5.4pt 0mm 5.4pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:black'>ATF2</span></span><span
+   style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span
    style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>　</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,673: / Line 1,652: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nsi1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nsi</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,689: / Line 1,669: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,695: / Line 1,675: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,715: / Line 1,695: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,721: / Line 1,701: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Pst1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,737: / Line 1,718: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,743: / Line 1,724: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,764: / Line 1,745: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅲ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>III</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1<span
@@ Line 1,773: / Line 1,754: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>gene
+of <span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i> </span></span>gene
 was purified.</span></p>
@@ Line 1,785: / Line 1,766: @@
    padding:0mm 5.4pt 0mm 5.4pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:black'>ATF2</span></span><span
+   style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span
    style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif";color:black'>　</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,796: / Line 1,777: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>EcoR1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Eco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>R1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,812: / Line 1,794: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,818: / Line 1,800: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,831: / Line 1,813: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅳ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>IV</span></p>
 <p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１</span></p>
@@ Line 1,839: / Line 1,821: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>gene
+of <span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i> </span></span>gene
 was purified.</span></p>
@@ Line 1,856: / Line 1,838: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,862: / Line 1,844: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>EcoR1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Eco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>R1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,878: / Line 1,861: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,884: / Line 1,867: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,896: / Line 1,879: @@
 <p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>26th August</span></b></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Cell free extract
+was prepared and applied to SDS-PAGE to detect <i>ATF2</i> protein.</span></p>
 <p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
@@ Line 1,958: / Line 1,946: @@
 <p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>29th August</span></b></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>I</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 1,971: / Line 1,959: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>２</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>II</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 2,013: / Line 2,001: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,019: / Line 2,007: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nsi1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nsi</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,035: / Line 2,024: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,041: / Line 2,030: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,061: / Line 2,050: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,067: / Line 2,056: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Pst1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,083: / Line 2,073: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,089: / Line 2,079: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,110: / Line 2,100: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR
 to amplify it.</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>&nbsp;</span></b></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>&nbsp;</span></b></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Cell free extract
+was prepared with FastBreak Cell Lysis Reagent and applied to SDS-PAGE.</span></p>
 <p class=MsoNormal><b><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></b></p>
@@ Line 2,115: / Line 2,112: @@
 <p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>30th August</span></b></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅰ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>I</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 2,156: / Line 2,153: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,162: / Line 2,159: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nsi1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nsi</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,178: / Line 2,176: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,184: / Line 2,182: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,204: / Line 2,202: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,210: / Line 2,208: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Pst1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,226: / Line 2,225: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,232: / Line 2,231: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,245: / Line 2,244: @@
 <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-style='font-size:11.0pt;color:windowtext'>ATF2</span></span><span lang=EN-US
+style='font-size:11.0pt;color:windowtext'><i>ATF2</i></span></span><span lang=EN-US
 style='font-size:11.0pt'> extracted from the Blue gel was purified.</span></p>
@@ Line 2,252: / Line 2,251: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
-carried out using the following primers for Psb1c3.</span></p>
+carried out using the following primers for pSB1C3.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR products were
@@ Line 2,258: / Line 2,257: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>gene
+of <span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i> </span></span>gene
 was purified.</span></p>
@@ Line 2,270: / Line 2,269: @@
    padding:0mm 5.4pt 0mm 5.4pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:black'>ATF2</span></span><span
+   style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span
    style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif";color:black'>　</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,281: / Line 2,280: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Pst1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,297: / Line 2,297: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,303: / Line 2,303: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,312: / Line 2,312: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>gene
+of <span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i> </span></span>gene
 was purified.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅱ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>II</span></p>
 <p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR (prepared
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR
-/29).</span></p>
+(prepared 8/29).</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 2,364: / Line 2,364: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,370: / Line 2,370: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nsi1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nsi</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,386: / Line 2,387: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,392: / Line 2,393: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,412: / Line 2,413: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,418: / Line 2,419: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Pst1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,434: / Line 2,436: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,440: / Line 2,442: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30ul</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,453: / Line 2,455: @@
 <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-style='font-size:11.0pt;color:windowtext'>ATF2</span></span><span lang=EN-US
+style='font-size:11.0pt;color:windowtext'><i>ATF2</i></span></span><span lang=EN-US
 style='font-size:11.0pt'> extracted from the Blue gel was purified.</span></p>
@@ Line 2,475: / Line 2,477: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We digested it and
-psb1c3 with <i>Xba</i>1 and <i>Spe</i>1.</span></p>
+cc3 with <i>Xba</i>1 and <i>Spe</i>1.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We electrophoresed
@@ Line 2,495: / Line 2,497: @@
 mutant of <i>ATF2</i>.</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Three tubes of
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Three tubes of pSB1C3
-psb1c3 were treated ethanol precipitation</span></p>
+were treated ethanol precipitation</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We add 25</span><span
 lang=EN-US style='font-size:11.0pt;font-family:Symbol'>m</span><span
-lang=EN-US style='font-size:11.0pt'>L H2O and digested it with <i>Xba</i>1 and <i>Spe</i>1.</span></p>
+lang=EN-US style='font-size:11.0pt'>L H<sub>2</sub>O and digested it with <i>Xba</i>1
+and <i>Spe</i>1.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We electrophoresed
@@ Line 2,518: / Line 2,521: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We did rapid check
-of the insert by colony cracking (<i>ATF2</i>+psb1c3).</span></p>
+of the insert by colony cracking (<i>ATF2</i>+pSB1C3).</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>But we detected no
@@ Line 2,533: / Line 2,536: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We cultured other
-sample (<i>ATF2</i>+psb1c3).</span></p>
+sample (<i>ATF2</i>+pSB1C3).</span></p>
 <p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+</div>
-</div>
 </div>