Team:KIT-Kyoto/Notebook/YJL

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Latest revision as of 08:46, 27 September 2013

YJL068C

13th August

We designed the PCR primers to amplify YJL068C gene.

19^th August

1. Make Primer mix

We added 102.2 µL and 100.3 µL H₂O to the forward and reverse primers, respectively, to make 100 pmol/µL solutions.

We obtained 10 µL of each primer solution and added 80 µL H₂O

to make the 10 pmol/µL primer mix.

2. PCR

We performed PCR to amplify the YJL068C gene.

Buffer	50µL
dNTP	20µL
Primer mix	1µL
Genome DNA	0.5µL
KOD-FX	2µL
H₂O	26.5µL

(Annealing : 52°C)

We detected two DNA bands around 1400bp and 3000bp.

(The correct band is 1400bp.)

We changed annealing temperature to 54°C and 57°C and tried PCR again.

We detected a correct size DNA band around 1400bp.

The clear DNA band was amplified at 54˚C (annealing temperature).

We performed PCR to amplify YJL068C gene at 54°C of annealing temp (total volume was 500 µL).

20^th August

1

We performed PCR to check whether the primers can work or not.

(Annealing temperature: 55°C)

We detected a DNA band of the correct size around 900bp.

2

We purified the YJL068C gene (PCR was performed on 8/19).

We digested YJL068C with BanII to check whether PCR succeeded or not.

We ligated YJL068C and pSB1C3.

YJL068C	24µL
M Buffer	3µL
BSA	1µL
XbaI	1µL
SpeI	1µL

YJL068C	6µL
H Buff.	2µL
H₂O	11µL
BanII	1µL

We digested pSB1C3 with XbaI and SpeI at 37°C for 90 minutes.

pSB1C3	49µL
M Buffer	5.78µL
BSA	1µL
XbaI	1µL
SpeI	1µL

We added 1µL BAP to dephosphorylate the pcb1c3 gene.

We electrophoresed the YJL068C gene and pSB1C3 (digested for ligation) in 1% Blue gel.

We saw the clear band of YJL068C, and not so clear band of pSB1C3.

They were extracted from the Blue gel.

We prepared the spare the spare in case of failure (made stocks of purified YJL068C and pSB1C3).

We purified YJL068C and pSB1C3 (these were in one tube) and ligated them.

We transformed it all (20 µL) into E.coli cells.

21th August

1

We checked the YJL068C gene (digested with Ban2 8/20) by electrophoresing.

We could see correct band (succeeded PCR).

2

We checked of the insert by colony cracking (transformed 8/20).

We couldn’t see clear bands, but cultured furthermore.

3.

We made the spare in case of failure (prepared 8/20).

We purified the YJL068C gene (digested 8/20).

We digested pSB1C3 with XbaI and SpeI (90 minutes at 37°C), and added 1µL BAP to it.

pSB1C3	96µL
Buffer	11µL
BSA	1µL
XbaI	1µL
SpeI	1µL

We electrophoresed pSB1C3 it in 1% Blue gel.

It was extracted from the Blue gel.

YJL068C	5µL
pSB1C3	5µL
Ligation Mix	10µL

We added 5 µL H₂O to dried YJL068C gene and pSB1C3 each.

We ligated them and transformed it into E.coli cells.

22th August

1.

We did miniprep to purify DNA.

We digested YJL068C in pSB1C3 with PstI and EcoRI, and incubated at 37°C for 90 minutes.

Plasmid DNA	10µL
H Buffer	2µL
H₂O	6µL
EcoRI	1µL
PstI	1µL

We applied them to 1% agarose gel electrophoresis and checked them.

5 samples showed the correct size.

2.

We digested pSB1C3 with BsiWI.

pSB1C3	10µL
NEB Buffer 3	2µL
BsiWI	1µL
H₂O	7µL

23th August

1.

We applied YJL068C in pSB1C3 (prepared on 8/22) to 1% agarose gel electrophoresis.

We detected the correct size of DNA.

Each transformant was cultured in LB liquid medium with chloramphenicol.

Plasmid DNA was purified by miniprep.

24th August

We added 29.5µL of H₂O to purified YJL068C.

We digested YJL068C with BanII and checked.

YJL068C	5µL
H Buff.	2µL
H₂O	12µL
BanII	1µL

We detected the correct size of DNA band.

We digested YJL068C with SpeI and BamHI.

YJL068C	24.5µL
Buffer	3µL
BSA	0.5µL
SpeI	1µL
BamHI	1µL

We digested pET-15b with XbaI and BamHI and dephosphorylated with 1µL BAP.

pET-15b	87µL
Buffer	10µL
BSA	1µL
XbaI	1µL
BamHI	1µL

We extracted DNA from the Blue gel.

We added 10 µL H₂O to dried YJL068C gene and pET-15b.

We ligated them and transformed it into E.coli cells.

25th August

We cultured them furthermore.

26th August

We checked the insertion of DNA by colony cracking.

We cultured each transformant in LB liquid medium with ampicillin.

We added 20ul of IPTG to them and incubated.

Cell free extract was prepared and applied to SDS-PAGE to detect YJL068C protein.

29th August

Cell free extract was prepared with FastBreak Cell Lysis Reagent and applied to SDS-PAGE.

30th August

We performed PCR to amplify YJL068C.

(Annealing temperature: 55°C)

31th August

We purified the YJL068C (performed PCR at 8/30).

We digested YJL068C with BanII to check whether PCR succeeded or not.

We ligated YJL068C and pSB1C3.

We digested YJL068C with XhoI and NdeI for ligation.

We purified pET-15b and digested with XhoI and NdeI.

YJL068C	24µL
Buffer	3µL
BSA	1µL
XhoI	1µL
NdeI	1µL

pET-15b	33µL
Buffer	4µL
BSA	1µL
XhoI	1µL
NdeI	1µL

DNA samples were applied to 1% Blue gel electrophoresis and then purified.

We added 10 µL H₂O to dried YJL068C gene and pET-15b.

We ligated them and transformed it into E.coli cells.

September 1^st

Picked 32 colonies of YJL068C transformants.

September 4th

Digested YJL068C and pET-15b overnight.

Applied pET-15b and YJL068C to the blue gel electrophoresis.

Isolated and purified it.

September 5^th

Purified PCR products and dissolved 24ul of H₂O.

Purified pET-15b.

Digested YJL068C and pET-15b with XhoI and NdeI overnight.

YJL068C	24µL
Buffer	3µL
BSA	1µL
XhoI	1µL
NdeI	1µL

pET-15b	33µL
Buffer	4µL
BSA	1µL
XhoI	1µL
NdeI	1µL

September 6th

1.

Applied YJL068C and pET-15b(prepared on September 5th) to the blue gel electrophoresis.

Isolated and purified it.

Ligation of pET-15b with YJL 068C (prep).

YJL068C	10µL
pET-15b	10µL
Ligation Mix	10µL

Transformed YJL068C into pET-15b E. coli cells.

2.

Purified PCR products and pET-15b were dissolved in 24.5ul of H₂O.

YJL068C and pET-15b were digested with XhoI and NdeI(overnight).

YJL068C	24.5µL
Buffer	3µL
BSA	0.5µL
XhoI	1µL
NdeI	1µL

pET-15b	24.5µL
Buffer	3µL
BSA	0.5µL
XhoI	1µL
NdeI	1µL

Applied YJL068C and pET-15b(prepared on September 5th) to the blue gel electrophoresis.

Isolated and purified it.

Ligation pET-15b and YJL068C (prep).

Transformed YJL068C into pET-15b E. coli cells.

September 7^th

Picked 48 colonies of YJL068C transformants.

Checked the colonies by colony cracking.

Picked up the appropriate colonies and cultured in 3ml LB medium with ampicillin.

September 8^th

Plasmid DNA was prepared by miniprep and digested with XhoI/NdeI.

(1)

Plasmid DNA	15.5µL
Buffer	2µL
BSA	0.5µL
XhoI	1µL
NdeI	1µL

(2)

BsiWI	1µL
Buffer	2µL
Plasmid DNA	17µL

Electrophoresed in 1% agarose gel.

Not succeeded.

September 15^th

Transformed YJL068C into pET-15b E. coli cells.

September 16^th

Picked 48 colonies of YJL068C transformants.

September 17th

Checked the colonies by colony cracking.

Picked up the colonies and cultured in 3ml LB medium with ampicillin.

September 18^th

Miniprepped plasmid DNA(YJL068C into pET-15b) and digested it.

Electrophoresed in 1% agarose gel.

Not succeeded.

September 19^th

YJL068C and pET-15b were digested with XhoI and BlpI.

YJL068C	24.5µL
NEB Buffer 4	3µL
BSA	0.5µL
XhoI	1µL
BlpI	1µL

pET-15b	24.5µL
NEB Buffer 4	3µL
BSA	0.5µL
XhoI	1µL
BlpI	1µL

Transformed YJL068C into pET-15b E. coli cells.

September 20^th

Picked 64 colonies of YJL068C transformants.

September 21st

Checked the colonies by colony cracking.

Picked up the appropriate colonies and cultured in the LB in 3ml medium with ampicillin.

September 22^nd

Miniprepped plasmid DNA(YJL068C into pET-15b) and digested it.

Plasmid DNA	16µL
XhoI	1µL
BlpI	1µL
NEB Buffer 4	2µL

Electrophoresed in 1% agarose gel.

We could not get any YJL068C into pET-15b E. coli cells.

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@@ Line 49: / Line 17: @@
-<div class=WordSection1 style='layout-grid:18.0pt'>
 <p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>13th August</span></b></p>
@@ Line 68: / Line 34: @@
 </span></span><span lang=EN-US style='font-size:11.0pt'>Make Primer mix</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We added 102.2</span><span
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We added 102.2 &micro;L
-lang=EN-US style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
+and 100.3 &micro;L H<sub>2</sub>O to the forward and reverse primers, respectively, to
-.0pt'>L and 100.3</span><span lang=EN-US style='font-size:11.0pt'>&micro;</span><span
+make 100 pmol/&micro;L solutions.</span></p>
-lang=EN-US style='font-size:11.0pt'>L H<sub>2</sub>O to the forward and reverse
-primers, respectively, to make 100 pmol/</span><span lang=EN-US
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We obtained 10 &micro;L
-style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:11.0pt'>L
+of each primer solution and added 80 &micro;L H<sub>2</sub>O</span></p>
-solutions.</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We obtained 10</span><span
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;to make the
-lang=EN-US style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
+pmol/&micro;L primer mix.</span></p>
-.0pt'>L of each primer solution and added 80</span><span lang=EN-US
-style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:11.0pt'>L H<sub>2</sub>O
-to make the 10 pmol/</span><span lang=EN-US style='font-size:11.0pt'>&micro;</span><span
-lang=EN-US style='font-size:11.0pt'>L primer mix.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
@@ Line 101: / Line 62: @@
    <td width=162 valign=top style='width:121.45pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>50</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>50&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 114: / Line 73: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>20</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>20&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 127: / Line 84: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 140: / Line 95: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 153: / Line 106: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 166: / Line 117: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>26.5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>26.5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 174: / Line 123: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;(Annealing :
-</span><span lang=EN-US> </span><span lang=EN-US style='font-size:11.0pt'>&#730;C)</span></p>
+°C)</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We detected two
@@ Line 183: / Line 132: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We changed
-annealing temperature to 54°C or 57</span><span lang=EN-US> </span><span
+annealing temperature to 54°C and 57°C and tried PCR again.</span></p>
-lang=EN-US style='font-size:11.0pt'>&#730;C and tried PCR again.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We detected a
@@ Line 190: / Line 138: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The clear DNA band
-was amplified at 54</span><span lang=EN-US> </span><span lang=EN-US
+was amplified at 54&#730;C (annealing temperature).</span></p>
-style='font-size:11.0pt'>&#730;C of annealing temperature.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR
-to amplify <i>YJL068C</i> gene at 54°C of annealing temp (total volume was 500</span><span
+to amplify <i>YJL068C</i> gene at 54°C of annealing temp (total volume was 500 &micro;L).</span></p>
-lang=EN-US style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L).</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
@@ Line 256: / Line 201: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 269: / Line 212: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 282: / Line 223: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 290: / Line 229: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>XbaI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xba</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 303: / Line 241: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>SpeI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Spe</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 326: / Line 263: @@
    <td width=56 valign=top style='width:41.65pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>6</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>6&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 339: / Line 274: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 347: / Line 280: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>H2O</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>H<sub>2</sub>O</span></p>
    </td>
    <td width=56 valign=top style='width:41.65pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>11</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>11&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 360: / Line 291: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BanII</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Ban</span></i><span
+  lang=EN-US style='font-size:11.0pt'>II</span></p>
    </td>
    <td width=56 valign=top style='width:41.65pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 374: / Line 304: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We digested psb1c3
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We digested pSB1C3
-with XbaI and SpeI at 37°C for 90 minutes.</span></p>
+with <i>Xba</i>I and <i>Spe</i>I at 37°C for 90 minutes.</span></p>
 <table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
@@ Line 386: / Line 316: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>49</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>49&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 399: / Line 327: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5.78</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5.78&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 412: / Line 338: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 426: / Line 350: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 434: / Line 356: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>SpeI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Spe</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
 </table>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We added 1</span><span
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We added 1&micro;L BAP
-lang=EN-US style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
+to dephosphorylate the <i>pcb1c3</i> gene.</span></p>
-.0pt'>L BAP to dephosphorylate the <i>pcb1c3</i> gene.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We electrophoresed
-the <i>YJL068C</i> gene<i> </i>and psb1c3 (digested for ligation) in 1% Blue gel.</span></p>
+the <i>YJL068C</i> gene<i> </i>and pSB1C3 (digested for ligation) in 1% Blue
+gel.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We saw the clear
-band of YJL068C, and not so clear band of psb1c3.</span></p>
+band of YJL068C, and not so clear band of pSB1C3.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>They were
@@ Line 460: / Line 381: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We prepared the spare
-the spare in case of failure (made stocks of purified YJL068C and psb1c3).</span></p>
+the spare in case of failure (made stocks of purified YJL068C and pSB1C3).</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We purified
-YJL068C and psb1c3 (these were in one tube) and ligated them.</span></p>
+YJL068C and pSB1C3 (these were in one tube) and ligated them.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We transformed it
-all (20</span><span lang=EN-US style='font-size:11.0pt'>&micro;</span><span
+all (20 &micro;L) into <i>E.coli</i> cells.</span></p>
-lang=EN-US style='font-size:11.0pt'>L) into <i>E.coli</i> cells.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
@@ Line 504: / Line 424: @@
 gene (digested 8/20).</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We digested psb1c3
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We digested pSB1C3
-with <i>Xba</i>I and <i>Spe</i>I (90 minutes at 37°C), and added 1</span><span
+with <i>Xba</i>I and <i>Spe</i>I (90 minutes at 37°C), and added 1&micro;L BAP to it.</span></p>
-lang=EN-US style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L BAP to it.</span></p>
 <table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
@@ Line 518: / Line 436: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>96</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>96&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 531: / Line 447: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>11</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>11&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 544: / Line 458: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 558: / Line 470: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 566: / Line 476: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>SpeI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Spe</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 579: / Line 488: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We electrophoresed
-psb1c3 it in 1% Blue gel.</span></p>
+pSB1C3 it in 1% Blue gel.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>It was extracted
@@ Line 593: / Line 502: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 606: / Line 513: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 619: / Line 524: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
 </table>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We added 5</span><span
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We added 5 &micro;L H<sub>2</sub>O
-lang=EN-US style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
+to dried <i>YJL068C</i> gene and pSB1C3 each.</span></p>
-.0pt'>L H<sub>2</sub>O to dried <i>YJL068C</i> gene and psb1c3 each.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We ligated them
@@ Line 645: / Line 547: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We digested <i>YJL068C</i>
-in pSB1C3 with PstI and EcorI, and incubated at 37°C for 90 minutes.</span></p>
+in pSB1C3 with <i>Pst</i>I and <i>Eco</i>RI, and incubated at 37°C for 90
+minutes.</span></p>
 <table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
@@ Line 656: / Line 559: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 669: / Line 570: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 682: / Line 581: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>6</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>6&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 690: / Line 587: @@
    <td width=120 valign=top style='width:89.9pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>EcoRI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Eco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>RI</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 703: / Line 599: @@
    <td width=120 valign=top style='width:89.9pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PstI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 726: / Line 621: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We digested pSB1C3
-with BsiW1.</span></p>
+with <i>Bsi</i>WI.</span></p>
 <table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
@@ Line 737: / Line 632: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 750: / Line 643: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 758: / Line 649: @@
    <td width=107 valign=top style='width:80.1pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BsiWI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bsi</span></i><span
+  lang=EN-US style='font-size:11.0pt'>WI</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 776: / Line 666: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>7</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>7&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 798: / Line 686: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Each transformant
-was cultured in LB liquid medium containing Chloramphenicol.</span></p>
+was cultured in LB liquid medium with chloramphenicol.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Plasmid DNA was
@@ Line 809: / Line 697: @@
 <p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>24th August</span></b></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We added 29.5</span><span
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We added 29.5&micro;L of
-lang=EN-US style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
+H<sub>2</sub>O to purified YJL068C.</span></p>
-.0pt'>L of H<sub>2</sub>O to purified YJL068C.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We digested <i>YJL068C</i>
-with BanII and checked.</span></p>
+with <i>Ban</i>II and checked.</span></p>
 <table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
@@ Line 825: / Line 712: @@
    <td width=56 valign=top style='width:41.65pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 838: / Line 723: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 851: / Line 734: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>12</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>12&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 859: / Line 740: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BanII</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Ban</span></i><span
+  lang=EN-US style='font-size:11.0pt'>II</span></p>
    </td>
    <td width=56 valign=top style='width:41.65pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 875: / Line 755: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We digested<i>
-YJL068C </i>with SpeI and BamHI.</span></p>
+YJL068C </i>with <i>Spe</i>I and <i>Bam</i>HI.</span></p>
 <table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
@@ Line 886: / Line 766: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24.5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24.5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 899: / Line 777: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 912: / Line 788: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 920: / Line 794: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>SpeI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Spe</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 933: / Line 806: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BamHI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bam</span></i><span
+  lang=EN-US style='font-size:11.0pt'>HI</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 946: / Line 818: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We digested
-pET-15b with XbaI and BamHI and dephosphorylated with 1uL BAP.</span></p>
+pET-15b with <i>Xba</i>I and <i>Bam</i>HI and dephosphorylated with 1&micro;L BAP.</span></p>
 <table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
@@ Line 957: / Line 829: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>87</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>87&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 970: / Line 840: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 983: / Line 851: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 991: / Line 857: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>XbaI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xba</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,004: / Line 869: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BamHI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bam</span></i><span
+  lang=EN-US style='font-size:11.0pt'>HI</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,019: / Line 883: @@
 from the Blue gel.</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We added 10</span><span
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We added 10 &micro;L H<sub>2</sub>O
-lang=EN-US style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
+to dried <i>YJL068C</i> gene and pET-15b.</span></p>
-.0pt'>L H<sub>2</sub>O to dried <i>YJL068C</i> gene and pET-15b.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We ligated them
@@ Line 1,041: / Line 904: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We cultured each
-transformant in LB liquid medium containing ampicillin.</span></p>
+transformant in LB liquid medium with ampicillin.</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We added 20</span><span
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We added 20ul of
-lang=EN-US style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
+IPTG to them and incubated.</span></p>
-.0pt'>L of IPTG to them and incubated.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Cell free extract
-was prepared and applied to SDS-PAGE to detecte ATF2 protein.</span></p>
+was prepared and applied to SDS-PAGE to detect <i>YJL068C </i>protein.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
@@ Line 1,101: / Line 963: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,114: / Line 974: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,127: / Line 985: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,135: / Line 991: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>XhoI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,148: / Line 1,003: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>NdeI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nde</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,171: / Line 1,025: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>33</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>33&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,184: / Line 1,036: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>4</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>4&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,197: / Line 1,047: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,205: / Line 1,053: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>XhoI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,218: / Line 1,065: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>NdeI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nde</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,235: / Line 1,081: @@
 applied to 1% Blue gel electrophoresis and then purified.</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We added 10</span><span
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We added 10 &micro;L H<sub>2</sub>O
-lang=EN-US style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
+to dried <i>YJL068C</i> gene and pET-15b.</span></p>
-.0pt'>L H<sub>2</sub>O to dried <i>YJL068C</i> gene and pET-15b.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We ligated them
@@ Line 1,261: / Line 1,106: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Applied pET-15b
-and <i>ATF1</i> to the blue gel electrophoresis.</span></p>
+and <i>YJL068C</i> to the blue gel electrophoresis.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Isolated and purified
@@ Line 1,273: / Line 1,118: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Purified PCR
-products and dissolved 24&micro;L of H<sub>2</sub>O.</span></p>
+products and dissolved 24ul of H<sub>2</sub>O.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Purified pET-15b.</span></p>
@@ Line 1,280: / Line 1,125: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Digested <i>YJL068C</i>
-and pET-15b with XhoI and NdeI overnight.</span></p>
+and pET-15b with <i>Xho</i>I and <i>Nde</i>I overnight.</span></p>
 <table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
@@ Line 1,291: / Line 1,136: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,304: / Line 1,147: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,317: / Line 1,158: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,325: / Line 1,164: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>XhoI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,338: / Line 1,176: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>NdeI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nde</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,361: / Line 1,198: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>33</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>33&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,374: / Line 1,209: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>4</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>4&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,387: / Line 1,220: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,395: / Line 1,226: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>XhoI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,408: / Line 1,238: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>NdeI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nde</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,453: / Line 1,282: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,466: / Line 1,293: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,479: / Line 1,304: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,494: / Line 1,317: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Purified PCR
-products and pET-15b were dissolved in 24.5</span><span lang=EN-US
+products and pET-15b were dissolved in 24.5ul of H<sub>2</sub>O.</span></p>
-style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:11.0pt'>L of
-H<sub>2</sub>O.</span></p>
 <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>YJL068C</span></i><span
-lang=EN-US style='font-size:11.0pt'> and pET-15b were digested with Xho</span><span
+lang=EN-US style='font-size:11.0pt'> and pET-15b were digested with <i>Xho</i>I
-style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅰ</span><span lang=EN-US
+and <i>Nde</i>I(overnight).</span></p>
-style='font-size:11.0pt'>and Nde</span><span style='font-size:11.0pt;
-font-family:"ＭＳ 明朝","serif"'>Ⅰ</span><span lang=EN-US style='font-size:11.0pt'>(overnight).</span></p>
 <table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
@@ Line 1,513: / Line 1,332: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24.5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24.5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,526: / Line 1,343: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,539: / Line 1,354: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,547: / Line 1,360: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>XhoI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,560: / Line 1,372: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>NdeI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nde</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,583: / Line 1,394: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24.5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24.5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,596: / Line 1,405: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,609: / Line 1,416: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,617: / Line 1,422: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>XhoI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,630: / Line 1,434: @@
    <td width=90 valign=top style='width:67.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>NdeI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nde</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,648: / Line 1,451: @@
 purified it.</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Ligation of
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Ligation pET-15b
-pET-15b with YJL (prep).</span></p>
+and <i>YJL068C</i> (prep).</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Transformed YJL<i>068C</i>
@@ Line 1,667: / Line 1,470: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Picked up the
-appropriate colonies and cultured in 3mL LB medium with ampicillin.</span></p>
+appropriate colonies and cultured in 3ml LB medium with ampicillin.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
@@ Line 1,681: / Line 1,484: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Plasmid DNA was
-prepared by miniprep and digested with XhoI/NdeI.</span></p>
+prepared by miniprep and digested with <i>Xho</i>I/<i>Nde</i>I.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(1)</span></p>
@@ Line 1,694: / Line 1,497: @@
    <td width=57 valign=top style='width:42.4pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>15.5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>15.5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,707: / Line 1,508: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,720: / Line 1,519: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,728: / Line 1,525: @@
    <td width=120 valign=top style='width:89.9pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>XhoI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=57 valign=top style='width:42.4pt;border-top:none;border-left:none;
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,741: / Line 1,537: @@
    <td width=120 valign=top style='width:89.9pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>NdeI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nde</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=57 valign=top style='width:42.4pt;border-top:none;border-left:none;
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,760: / Line 1,555: @@
    <td width=120 valign=top style='width:89.9pt;border:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BsiWI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bsi</span></i><span
+  lang=EN-US style='font-size:11.0pt'>WI</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,777: / Line 1,571: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,790: / Line 1,582: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>17</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>17&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,830: / Line 1,620: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Picked up the
-colonies and cultured in 3mL LB medium with ampicillin.</span></p>
+colonies and cultured in 3ml LB medium with ampicillin.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
@@ Line 1,864: / Line 1,654: @@
 <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>YJL068C</span></i><span
-lang=EN-US style='font-size:11.0pt'> and pET-15b were digested with XhoI and
+lang=EN-US style='font-size:11.0pt'> and pET-15b were digested with <i>Xho</i>I
-BlpI .</span></p>
+and <i>Blp</i>I.</span></p>
 <table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
@@ Line 1,876: / Line 1,666: @@
    <td width=57 valign=top style='width:42.4pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24.5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24.5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,889: / Line 1,677: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,902: / Line 1,688: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,910: / Line 1,694: @@
    <td width=119 valign=top style='width:89.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>XhoI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=57 valign=top style='width:42.4pt;border-top:none;border-left:none;
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,923: / Line 1,706: @@
    <td width=119 valign=top style='width:89.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BlpI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Blp</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=57 valign=top style='width:42.4pt;border-top:none;border-left:none;
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,946: / Line 1,728: @@
    <td width=57 valign=top style='width:42.4pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24.5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24.5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,959: / Line 1,739: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,972: / Line 1,750: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,980: / Line 1,756: @@
    <td width=119 valign=top style='width:89.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>XhoI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=57 valign=top style='width:42.4pt;border-top:none;border-left:none;
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 1,993: / Line 1,768: @@
    <td width=119 valign=top style='width:89.3pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BlpI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Blp</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=57 valign=top style='width:42.4pt;border-top:none;border-left:none;
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 2,027: / Line 1,801: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Picked up the
-appropriate colonies and cultured in the LB in 3mL ampicillin(+) medium.</span></p>
+appropriate colonies and cultured in the LB in 3ml medium with ampicillin.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
@@ Line 2,047: / Line 1,821: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>16</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>16&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 2,055: / Line 1,827: @@
    <td width=120 valign=top style='width:89.9pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>XhoI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 2,068: / Line 1,839: @@
    <td width=120 valign=top style='width:89.9pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BlpI</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Blp</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 2,086: / Line 1,856: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2</span><span
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
-  lang=ja style='font-size:11.0pt'>&micro;</span><span lang=EN-US style='font-size:
-.0pt'>L</span></p>
    </td>
   </tr>
@@ Line 2,102: / Line 1,870: @@
 </div>
 </div>
-</html>