Team:Hong Kong HKUST/module3backup
From 2013.igem.org
(Difference between revisions)
(5 intermediate revisions not shown) | |||
Line 376: | Line 376: | ||
</li> | </li> | ||
- | + | <li> | |
+ | <a href=#4>Reference</a> | ||
+ | |||
+ | </li> | ||
Line 410: | Line 413: | ||
<div class="nine columns"><p id="1"></p> | <div class="nine columns"><p id="1"></p> | ||
<h3>Overview</h3> | <h3>Overview</h3> | ||
- | In | + | In nature, eukaryotic cell mitochondria usually have their protein encoded by gene in nucleus and produced from cytosol. In this process, MLS will act as signal peptide to target the protein into mitochondria. |
+ | <br> | ||
+ | In our module, we will construct the MLS BioBrick <a href="http://parts.igem.org/Part:BBa_K1119001">K1119001</a>, and characterize it quantitatively[link]. | ||
<br> | <br> | ||
</div> | </div> | ||
Line 417: | Line 422: | ||
<div class="nine columns"><p id="2"></p> | <div class="nine columns"><p id="2"></p> | ||
<h3>Mechanism of MLS</h3> | <h3>Mechanism of MLS</h3> | ||
- | MLS is attached to the N-terminal of the protein in interest, and be recognized by receptor in the outer | + | MLS is attached to the N-terminal of the protein in interest, and be recognized by receptor in the outer mitochondria membrane. The complex of receptor and attached protein diffuse to a contact site, where protein is translocated across both the outer and inner membrane by a protein translocator. The MLS will be cleaved off by signal peptidase inside the mitochondria afterward. Chaperone proteins will help to pull the protein across the membrane and help the refolding of protein. |
- | https://static.igem.org/mediawiki/2013/8/87/MLS_mechanism.png | + | <img src="https://static.igem.org/mediawiki/2013/8/87/MLS_mechanism.png" > |
</div> | </div> | ||
</div> | </div> | ||
<div class="row"> | <div class="row"> | ||
<div class="nine columns"><p id="3"></p> | <div class="nine columns"><p id="3"></p> | ||
- | + | <h3>Linkage to project</h3> | |
- | + | In our project, we introduced bacterial enzymes to mammalian cell to modify metabolic pathway. However, unlike bacteria, citric acid cycle in mammalian cells is compartmentalized in mitochondria. The ACE proteins should be targeted to mitochondria for their functionality. To do so, we fused ACE enzymes with Mitochondrial Leader Sequence (MLS). | |
- | + | <br> | |
- | + | </div> | |
- | + | ||
</div> | </div> | ||
<div class="row"> | <div class="row"> | ||
- | <div class="nine columns"><p id=" | + | <div class="nine columns"><p id="4"></p> |
<h3>Reference</h3> | <h3>Reference</h3> | ||
- | Alberts, B., Bray, D., Hopkin, K., Johnson, A., Lewis, J., Raff, M., Roberts, K., & Walter, P. (2010). Essential cell biology. (3rd ed., p. 505). UK: Garland Science. | + | Alberts, B., Bray, D., Hopkin, K., Johnson, A., Lewis, J., Raff, M., Roberts, K., & Walter, P. (2010). Essential cell biology. (3rd ed., p. 505). UK: Garland Science. |
+ | |||
</div> | </div> | ||
</div> | </div> |
Latest revision as of 15:13, 27 September 2013
![](https://static.igem.org/mediawiki/igem.org/c/c7/BANNER1_%281%29.png)
-
Protein Trafficking
- Overview
- Mechanism of MLS
- Linkage to Project
- Reference
-
Modules
- FA Quantification & Cell Viability
- FA Sensing Mechanism
- Protein Trafficking
- Glyoxylate Shunt
Protein Trafficking
Overview
In nature, eukaryotic cell mitochondria usually have their protein encoded by gene in nucleus and produced from cytosol. In this process, MLS will act as signal peptide to target the protein into mitochondria.In our module, we will construct the MLS BioBrick K1119001, and characterize it quantitatively[link].
Mechanism of MLS
MLS is attached to the N-terminal of the protein in interest, and be recognized by receptor in the outer mitochondria membrane. The complex of receptor and attached protein diffuse to a contact site, where protein is translocated across both the outer and inner membrane by a protein translocator. The MLS will be cleaved off by signal peptidase inside the mitochondria afterward. Chaperone proteins will help to pull the protein across the membrane and help the refolding of protein.![](https://static.igem.org/mediawiki/2013/8/87/MLS_mechanism.png)