Team:Hong Kong HKUST/modules

From 2013.igem.org

(Difference between revisions)
 
(29 intermediate revisions not shown)
Line 15: Line 15:
   $(document).ready(function()
   $(document).ready(function()
   {
   {
-
   $('#tinkercell1-01').mouseover(function(){
+
   $('#cell-small-03').mouseover(function(){
-
     $('#interview').stop().show();
+
    $('.info1').stop().hide();
 +
     $('#presentation').stop().show();
   });
   });
-
   $('#tinkercell1-01').mouseleave(function(){
+
   $('#cell-small-06').mouseover(function(){
-
     $('#interview').stop().hide();
+
     $('.info1').stop().hide();
-
  });
+
-
  $('#tinkercell1-04').mouseover(function(){
+
     $('#article').stop().show();
     $('#article').stop().show();
   });
   });
-
   $('#tinkercell1-04').mouseleave(function(){
+
   $('#cell-small-09').mouseover(function(){
-
    $('#article').stop().hide();
+
     $('.info1').stop().hide();
-
  });
+
-
  $('#tinkercell1-07').mouseover(function(){
+
-
     $('#presentation').stop().show();
+
-
  });
+
-
  $('#tinkercell1-07').mouseleave(function(){
+
-
    $('#presentation').stop().hide();
+
-
  });
+
-
  $('#tinkercell1-08').mouseover(function(){
+
     $('#article2').stop().show();
     $('#article2').stop().show();
-
  });
 
-
  $('#tinkercell1-08').mouseleave(function(){
 
-
    $('#article2').stop().hide();
 
   });
   });
});
});
Line 59: Line 47:
</script>
</script>
-
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1">
+
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
-
<!-- Save for Web Styles (tinkercell1.psd) -->
+
<!-- Save for Web Styles (cell small.psd) -->
<style type="text/css">
<style type="text/css">
<!--
<!--
Line 67: Line 55:
position:absolute;
position:absolute;
left:22px;
left:22px;
-
top:372px;
+
top:375px;
width:600px;
width:600px;
height:400px;
height:400px;
-
       
 
}
}
-
#tinkercell1-01 {
+
#cell-small-01 {
position:absolute;
position:absolute;
left:0px;
left:0px;
top:0px;
top:0px;
-
width:145px;
+
width:600px;
-
height:160px;
+
height:128px;
}
}
-
#tinkercell1-02 {
+
#cell-small-02 {
position:absolute;
position:absolute;
-
left:145px;
+
left:0px;
-
top:0px;
+
top:128px;
-
width:455px;
+
width:121px;
-
height:80px;
+
height:272px;
}
}
-
#tinkercell1-03 {
+
#cell-small-03 {
position:absolute;
position:absolute;
-
left:145px;
+
left:121px;
-
top:80px;
+
top:128px;
-
width:180px;
+
width:95px;
-
height:80px;
+
height:84px;
}
}
-
#tinkercell1-04 {
+
#cell-small-04 {
position:absolute;
position:absolute;
-
left:325px;
+
left:216px;
-
top:80px;
+
top:128px;
-
width:152px;
+
width:117px;
-
height:154px;
+
height:84px;
}
}
-
#tinkercell1-05 {
+
#cell-small-05 {
position:absolute;
position:absolute;
-
left:477px;
+
left:333px;
-
top:80px;
+
top:128px;
-
width:123px;
+
width:70px;
-
height:154px;
+
height:159px;
}
}
-
#tinkercell1-06 {
+
#cell-small-06 {
position:absolute;
position:absolute;
-
left:0px;
+
left:403px;
-
top:160px;
+
top:128px;
-
width:145px;
+
width:122px;
-
height:187px;
+
height:159px;
}
}
-
#tinkercell1-07 {
+
#cell-small-07 {
position:absolute;
position:absolute;
-
left:145px;
+
left:525px;
-
top:160px;
+
top:128px;
-
width:180px;
+
width:75px;
-
height:187px;
+
height:272px;
}
}
-
#tinkercell1-08 {
+
#cell-small-08 {
position:absolute;
position:absolute;
-
left:325px;
+
left:121px;
-
top:234px;
+
top:212px;
-
width:275px;
+
width:120px;
-
height:113px;
+
height:75px;
}
}
-
#tinkercell1-09 {
+
#cell-small-09 {
position:absolute;
position:absolute;
-
left:0px;
+
left:241px;
-
top:347px;
+
top:212px;
-
width:600px;
+
width:92px;
-
height:53px;
+
height:75px;
 +
}
 +
 
 +
#cell-small-10 {
 +
position:absolute;
 +
left:121px;
 +
top:287px;
 +
width:404px;
 +
height:113px;
}
}
-->
-->
</style>
</style>
 +
<style type="text/css">
<style type="text/css">
Line 250: Line 246:
{width:35%;height:auto; border:2px solid;
{width:35%;height:auto; border:2px solid;
border-radius:15px;padding:10px;margin-left:20px;display:none;background-color:#35BB91;font-family:"Trebuchet MS", Helvetica, sans-serif;}
border-radius:15px;padding:10px;margin-left:20px;display:none;background-color:#35BB91;font-family:"Trebuchet MS", Helvetica, sans-serif;}
-
#interview
 
-
{position:absolute;top:345px;left:610px;}
 
#presentation
#presentation
-
{position:absolute;top:430px;left:610px;}
+
{position:absolute;top:625px;left:610px;}
#article
#article
-
{position:absolute;top:520px;left:610px;}
+
{position:absolute;top:490px;left:610px;}
#article2
#article2
-
{position:absolute;top:610px;left:610px;}
+
{position:absolute;top:380px;left:610px;}
#projinteract{margin-top:10px;width:60%;float:left;height:400px; border:2px solid;
#projinteract{margin-top:10px;width:60%;float:left;height:400px; border:2px solid;
-
border-radius:15px;padding:5px;background:#FFEBC8;margin-left:15px;}
+
border-radius:15px;padding:5px;background:#ffffff;margin-left:15px;}
#moddesc{margin-top:10px;width:96%;float:left;height:auto; border:2px solid;padding:10px;background:#ffffff;margin-left:20px;border-radius:15px;font-family:"Trebuchet MS", Helvetica, sans-serif;}
#moddesc{margin-top:10px;width:96%;float:left;height:auto; border:2px solid;padding:10px;background:#ffffff;margin-left:20px;border-radius:15px;font-family:"Trebuchet MS", Helvetica, sans-serif;}
#art-desc,#pre-desc,#int-desc{width:45%;float:right;margin-right:8px;}
#art-desc,#pre-desc,#int-desc{width:45%;float:right;margin-right:8px;}
Line 268: Line 262:
h3.title{font-size:28px;color:#35BB91;}
h3.title{font-size:28px;color:#35BB91;}
h3.kotak{margin-left:0px;}
h3.kotak{margin-left:0px;}
 +
#iGEM_Logo{
 +
width:100px;
 +
height:80px;
 +
position:absolute;
 +
right:10px;
 +
top:60px;
 +
z-index:+15;
 +
}
 +
#hkust_Logo{
 +
width:60px;
 +
height:80px;
 +
position:absolute;
 +
right:110px;
 +
top:60px;
 +
z-index:+15;
 +
}
 +
</style>
</style>
</head>
</head>
<body>
<body>
 +
 
 +
          <a href="https://2013.igem.org/Main_Page"><img id="iGEM_Logo" src="https://static.igem.org/mediawiki/2013/4/46/Igem_qgem_logo.png"></a>
 +
         
 +
 +
<a href="http://www.ust.hk/eng/index.htm"><img id="hkust_Logo" src="https://static.igem.org/mediawiki/2013/5/55/Hkust_logo.gif"></a>
 +
<a href=https://2013.igem.org/Team:Hong_Kong_HKUST><center><div id="kepala"><img src="https://static.igem.org/mediawiki/igem.org/c/c7/BANNER1_%281%29.png" style="height:121px;width:100%;"></div></center></a>
<a href=https://2013.igem.org/Team:Hong_Kong_HKUST><center><div id="kepala"><img src="https://static.igem.org/mediawiki/igem.org/c/c7/BANNER1_%281%29.png" style="height:121px;width:100%;"></div></center></a>
Line 302: Line 319:
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Wetlab">Wetlab</a>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Wetlab">Wetlab</a>
<ul>
<ul>
-
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment">Experiments</a></li>
 
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/notebook">Notebook</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/notebook">Notebook</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/protocols">Protocols</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/protocols">Protocols</a></li>
Line 331: Line 347:
</div>
</div>
-
<br><br><br><div id="slide"><h3 class="title">Project Outline</h3><p id="isi">The design of our project is to build a constitutive and inducible glyoxylate shunt that increases energy metabolism by accelerating fatty acid uptake rate. First, the glyxylate shunt is introduced into mammalian cell by two bacterial native genes, aceA and aceB. However, unlike bacteria, citric acid cycle occurs in mitochondria for mammals. Thus, we translocated glyoxylate enzymes into mitochondria by fusing them with mitochondrial leader sequence. Lastly, for the glyoxylate enzymes to be expressed constitutively, we have fused them with mammalian constitutive promoters, namely CMV and EF-1alpha promoters. For inducible circuit, the glyoxylate enzymes are designed to be fused with fatty acid inducible promoters such as fatty acid binding protein promoter. <strong>Hover</strong> your mouse and <strong>click</strong> on the images below to learn more about each modules!</p></div>
+
 
 +
<br><br><br><div id="slide"><h3 class="title">Project Outline</h3><p id="isi">Our ultimate goal is to build a ‘smart’ glyoxylate shunt that burns extra fatty acid when there is energy surplus. To achieve this, we would have to first build the shunt itself in mammalian cells. The shunt comprises two bacterial glyoxylate enzymes, isocitrate lyase (AceA) and malate synthase (AceB). Tagged with a localization signal peptide, they could be directed to the mitochondria and act on the citric acid cycle, eventually increasing fatty acid uptake. The two enzymes would be initially driven by constitutive promoters (CMV and EF-1alpha promoters), placing the shunt in a constantly “ON” state and burning calories regardless of the energy profile. To improve this, we are developing fatty acid responsive promoters, which, when used to regulate the glyoxylate enzymes, should dispense energy only when it is in excess. <strong>Hover</strong> your mouse and <strong>click</strong> on the images below to learn more about each modules!</p></div>
 +
 
 +
 
<div id="Table_01">
<div id="Table_01">
-
<div id="tinkercell1-01"> <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Project/module1"><img src="https://static.igem.org/mediawiki/igem.org/3/3c/Tinkercell1_01.jpg" width="145" height="160" alt=""></a>
+
<div id="cell-small-01">
 +
<img src="https://static.igem.org/mediawiki/2013/2/27/Cell-small_01.jpg" width="600" height="128" alt="">
</div>
</div>
-
<div id="tinkercell1-02">
+
<div id="cell-small-02">
-
<img src="https://static.igem.org/mediawiki/igem.org/c/cd/Tinkercell1_02.jpg" width="455" height="80" alt="">
+
<img src="https://static.igem.org/mediawiki/2013/e/e7/Cell-small_02.jpg" width="121" height="272" alt="">
</div>
</div>
-
<div id="tinkercell1-03">
+
<div id="cell-small-03"> <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Project/module2"><img src="https://static.igem.org/mediawiki/2013/4/4e/Cell-small_03.jpg" width="95" height="84" alt=""></a>
-
<img src="https://static.igem.org/mediawiki/igem.org/0/08/Tinkercell1_03.jpg" width="180" height="80" alt="">
+
</div>
</div>
-
<div id="tinkercell1-04"> <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Project/module3"><img src="https://static.igem.org/mediawiki/igem.org/5/5d/Tinkercell1_04.jpg" width="152" height="154" alt=""></a>
+
<div id="cell-small-04">
 +
<img src="https://static.igem.org/mediawiki/2013/d/d0/Cell-small_04.jpg" width="117" height="84" alt="">
</div>
</div>
-
<div id="tinkercell1-05">
+
<div id="cell-small-05">
-
<img src="https://static.igem.org/mediawiki/igem.org/5/56/Tinkercell1_05.jpg" width="123" height="154" alt="">
+
<img src="https://static.igem.org/mediawiki/2013/9/94/Cell-small_05.jpg" width="70" height="159" alt="">
</div>
</div>
-
<div id="tinkercell1-06">
+
<div id="cell-small-06"> <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Project/module3"><img src="https://static.igem.org/mediawiki/2013/6/68/Cell-small_06.jpg" width="122" height="159" alt=""></a>
-
<img src="https://static.igem.org/mediawiki/igem.org/0/00/Tinkercell1_06.jpg" width="145" height="187" alt="">
+
</div>
</div>
-
<div id="tinkercell1-07"> <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Project/module2"><img src="https://static.igem.org/mediawiki/igem.org/3/38/Tinkercell1_07.jpg" width="180" height="187" alt=""></a>
+
<div id="cell-small-07">
 +
<img src="https://static.igem.org/mediawiki/2013/b/be/Cell-small_07.jpg" width="75" height="272" alt="">
</div>
</div>
-
<div id="tinkercell1-08"> <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Project/module4"><img src="https://static.igem.org/mediawiki/igem.org/2/2e/Tinkercell1_08.jpg" width="275" height="113" alt=""></a>
+
<div id="cell-small-08">
 +
<img src="https://static.igem.org/mediawiki/2013/2/26/Cell-small_08.jpg" width="120" height="75" alt="">
</div>
</div>
-
<div id="tinkercell1-09">
+
<div id="cell-small-09"> <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Project/module4"><img src="https://static.igem.org/mediawiki/2013/d/d3/Cell-small_09.jpg" width="92" height="75" alt=""></a>
-
<img src="https://static.igem.org/mediawiki/igem.org/5/50/Tinkercell1_09.jpg" width="600" height="53" alt="">
+
</div>
 +
<div id="cell-small-10">
 +
<img src="https://static.igem.org/mediawiki/2013/9/9f/Cell-small_10.jpg" width="404" height="113" alt="">
</div>
</div>
-
 
</div>
</div>
 +
 +
<div id="projinteract"></div>
<div id="projinteract"></div>
-
<div id="interview"><h3 class="kotak">Cell Viability & Fatty Acid Quantification</h3><p><b><i>Responsible for:</i></b><br>Measuring cell viability at different fatty acid concentration & measure fatty acid uptake rate</i><br><b><i>Parts submitted:</b> - </i></p></div>
+
<div id="article2" class="info1"><h3 class="kotak">Glyoxylate Shunt</h3><p><b><i>Responsible for:</b></i><br>Introduce glyoxylate enzymes responsible for the shunt<br><b><i>Parts submitted:</b></i><a href="http://parts.igem.org/Part:BBa_K1119002">BBa_K1119002</a>, <a href="http://parts.igem.org/Part:BBa_K1119003">BBa_K1119003</a>, <a href="http://parts.igem.org/Part:BBa_K1119006">BBa_K1119006</a>, <a href="http://parts.igem.org/Part:BBa_K1119008">BBa_K1119008</a>, <a href="http://parts.igem.org/Part:BBa_K1119010">BBa_K1119010</a> & <a href="http://parts.igem.org/Part:BBa_K1119006">BBa_K1119011</a></p></div>
-
<div id="presentation"><h3 class="kotak">Fatty Acid Sensing Mechanism</h3><p><b><i>Responsible for:</b></i><br>Introduce inducible system that allows tunable fatty acid uptake by sensing fatty acid concentration<br><b><i>Parts submitted:</b> - </i></p></div>
+
<div id="article" class="info1"><h3 class="kotak">Protein Trafficking</h3><p><b><i>Responsible for:</b></i><br>Target ACE proteins into mitochondria<br><b><i>Parts submitted:</b></i> <a href="http://parts.igem.org/Part:BBa_K1119000">BBa_K1119000</a>, <a href="http://parts.igem.org/Part:BBa_K1119001">BBa_K1119001</a> & <a href="http://parts.igem.org/Part:BBa_K1119009">BBa_K1119009</a></p></div>
-
<div id="article"><h3 class="kotak">Protein Trafficking</h3><p><b><i>Responsible for:</b></i><br>Target ACE proteins into mitochondria<br><b><i>Parts submitted:</b></i> <a href="http://parts.igem.org/Part:BBa_K1119000">BBa_K1119000</a>, <a href="http://parts.igem.org/Part:BBa_K1119001">BBa_K1119001</a> & <a href="http://parts.igem.org/Part:BBa_K1119009">BBa_K1119009</a></p></div>
+
<div id="presentation" class="info1"><h3 class="kotak">Fatty Acid Sensing Mechanism</h3><p><b><i>Responsible for:</b></i><br>Introduce inducible system that allows tunable fatty acid uptake by sensing fatty acid concentration<br><b><i>Parts submitted:</b> - </i></p></div>
-
<div id="article2"><h3 class="kotak">Glyoxylate Shunt</h3><p><b><i>Responsible for:</b></i><br>Introduce glyoxylate enzymes responsible for the shunt<br><b><i>Parts submitted:</b></i><a href="http://parts.igem.org/Part:BBa_K1119002">BBa_K1119002</a>, <a href="http://parts.igem.org/Part:BBa_K1119003">BBa_K1119003</a>, <a href="http://parts.igem.org/Part:BBa_K1119004">BBa_K1119004</a>, <a href="http://parts.igem.org/Part:BBa_K1119006">BBa_K1119006</a> & <a href="http://parts.igem.org/Part:BBa_K1119008">BBa_K1119008</a></p></div>
+
</body>
</body>
</html>
</html>

Latest revision as of 12:05, 28 October 2013




Project Outline

Our ultimate goal is to build a ‘smart’ glyoxylate shunt that burns extra fatty acid when there is energy surplus. To achieve this, we would have to first build the shunt itself in mammalian cells. The shunt comprises two bacterial glyoxylate enzymes, isocitrate lyase (AceA) and malate synthase (AceB). Tagged with a localization signal peptide, they could be directed to the mitochondria and act on the citric acid cycle, eventually increasing fatty acid uptake. The two enzymes would be initially driven by constitutive promoters (CMV and EF-1alpha promoters), placing the shunt in a constantly “ON” state and burning calories regardless of the energy profile. To improve this, we are developing fatty acid responsive promoters, which, when used to regulate the glyoxylate enzymes, should dispense energy only when it is in excess. Hover your mouse and click on the images below to learn more about each modules!

Glyoxylate Shunt

Responsible for:
Introduce glyoxylate enzymes responsible for the shunt
Parts submitted:BBa_K1119002, BBa_K1119003, BBa_K1119006, BBa_K1119008, BBa_K1119010 & BBa_K1119011

Protein Trafficking

Responsible for:
Target ACE proteins into mitochondria
Parts submitted: BBa_K1119000, BBa_K1119001 & BBa_K1119009

Fatty Acid Sensing Mechanism

Responsible for:
Introduce inducible system that allows tunable fatty acid uptake by sensing fatty acid concentration
Parts submitted: -

Retrieved from "http://2013.igem.org/Team:Hong_Kong_HKUST/modules"