Team:IIT Madras

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{{:Team:IIT Madras/Templates/Header}}<html>
{{:Team:IIT Madras/Templates/Header}}<html>
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<div class="container marketing">
<div class="container marketing">
       <div id="myCarousel" class="carousel slide">
       <div id="myCarousel" class="carousel slide">
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       <ol class="carousel-indicators">
       <ol class="carousel-indicators">
         <li data-target="#myCarousel" data-slide-to="0" class="active"></li>
         <li data-target="#myCarousel" data-slide-to="0" class="active"></li>
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               <p>Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p>
               <p>Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p>
               <p><a class="btn btn-large btn-primary" href="#">More info</a></p>-->
               <p><a class="btn btn-large btn-primary" href="#">More info</a></p>-->
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<marquee behavior="scroll" direction="left" style="
<marquee behavior="scroll" direction="left" style="
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">COMBATING SHIGA TOXIN</marquee>
">COMBATING SHIGA TOXIN</marquee>
             </div>
             </div>
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       <a class="right carousel-control" href="#myCarousel" data-slide="next"><span class="glyphicon glyphicon-chevron-right"></span></a>
       <a class="right carousel-control" href="#myCarousel" data-slide="next"><span class="glyphicon glyphicon-chevron-right"></span></a>
     </div>
     </div>
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          <p><a class="btn btn-default" href="#">View details »</a></p>
 
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          <h2>Heading</h2>
 
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          <p>Duis mollis, est non commodo luctus, nisi erat porttitor ligula, eget lacinia odio sem nec elit. Cras mattis consectetur purus sit amet fermentum. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh.</p>
 
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          <img class="img-circle" src="data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAIwAAACMCAYAAACuwEE+AAAFC0lEQVR4Xu3XV0ukQRCF4TIgCAZUMIIJBQMKBvDWn64XillRVEyYLsScMevSDQ6mRc+O+8lY79ws61RNW6cevmmzjo6OnowXCXwxgSzAfDEpymICgAGClABgpLgoBgwGpAQAI8VFMWAwICUAGCkuigGDASkBwEhxUQwYDEgJAEaKi2LAYEBKADBSXBQDBgNSAoCR4qIYMBiQEgCMFBfFgMGAlABgpLgoBgwGpAQAI8VFMWAwICUAGCkuigGDASkBwEhxUQwYDEgJAEaKi2LAYEBKADBSXBQDBgNSAoCR4qIYMBiQEgCMFBfFgMGAlABgpLgoBgwGpAQAI8VFMWAwICUAGCkuigGDASkBwEhxUQwYDEgJAEaKi2LAYEBKADBSXBQDBgNSAoCR4qIYMBiQEgCMFBfFgMGAlABgpLgoBgwGpAQAI8VFMWAwICUAGCkuigGDASkBwEhxUQwYDEgJAEaKi2LAYEBKADBSXBQDBgNSAoCR4qIYMBiQEgCMFBfFGQ/m7u7OxsbGLD8/37q7u99tdHt725aWlqyjo8MqKiri+wcHB7aysmI3NzdWUlJira2tlpeX9yUNSZ/3pV8qwaKMBnN6emqLi4t2cXFhpaWl78CE9ycnJ+3x8TEFJiAZGhqyoqIiq6qqipgCpPb29k9jT/q8T3+hHyjIWDD39/c2MDBglZWVtr+/b8XFxa/AhCfB6OhofHKcnZ2lwOzs7Nj8/Hzq/1NTU/H9/v5+29zcjE+exsZGq62tjf0PDw/W19dnOTk5337eD+w77SMzFszT05NdXl5aQUGBDQ4OWmFh4SswMzMzFtCE5U9PT6eArK+v29ramvX09MSvo7m5Odvd3Y1gcnNzLQA6Pj6OT6zDw0Pr6uqysrIy+1/npb3BhD8gY8G8zOktmI2NDQswwp3m+vo6omhra4tfPVtbW6/AzM7O2t7eXgrM7e2tjYyMWPi3rq7Ompub363kO89LeN9pH/crwYyPj1u4b7x9tbS0WHZ2ti0sLFhnZ6eVl5fHO875+XkEE17hqRUu0eGr6KN7Uah5Cyad89LeYMIf8CvBnJycxIWHV8CwurpqDQ0NVlNTE382PDz84aU3XI7D8q+urqy6ujo+jZqamqy+vv7VWt6C+dfzEt71txz3K8G8TCbcQ17eYcJ7f/uzenl5OSIJf4KHp8/ExES8EPf29sZL9fProzvT83vKed+ywYQ/5FeASTgz18cBxvX69eEBo2fmugMwrtevDw8YPTPXHYBxvX59eMDombnuAIzr9evDA0bPzHUHYFyvXx8eMHpmrjsA43r9+vCA0TNz3QEY1+vXhweMnpnrDsC4Xr8+PGD0zFx3AMb1+vXhAaNn5roDMK7Xrw8PGD0z1x2Acb1+fXjA6Jm57gCM6/XrwwNGz8x1B2Bcr18fHjB6Zq47AON6/frwgNEzc90BGNfr14cHjJ6Z6w7AuF6/Pjxg9MxcdwDG9fr14QGjZ+a6AzCu168PDxg9M9cdgHG9fn14wOiZue4AjOv168MDRs/MdQdgXK9fHx4wemauOwDjev368IDRM3PdARjX69eHB4yemesOwLhevz48YPTMXHcAxvX69eEBo2fmugMwrtevDw8YPTPXHYBxvX59eMDombnuAIzr9evDA0bPzHUHYFyvXx8eMHpmrjsA43r9+vCA0TNz3QEY1+vXhweMnpnrDsC4Xr8+PGD0zFx3AMb1+vXh/wBs3s6mncQfmwAAAABJRU5ErkJggg==" data-src="holder.js/140x140" alt="140x140" style="width: 140px; height: 140px;">
 
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          <p>Donec sed odio dui. Cras justo odio, dapibus ac facilisis in, egestas eget quam. Vestibulum id ligula porta felis euismod semper. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus.</p>
 
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      <!-- START THE FEATURETTES -->
 
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           <h2 class="featurette-heading">First featurette heading. <span class="text-muted">It'll blow your mind.</span></h2>
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           Shiga toxin, a worldwide menace, has killed over 1 million people to date and continues to afflict almost 150 million people each year. Currently, there is no treatment for Shiga toxicosis and it leads to complications in the human system like hemolytic uremic syndrome (HUS) and renal failure. Here, we propose a two-fold, novel synthetic biology approach to combat the lethal effect of the toxin. We aim to neutralize the already produced toxin through a nine amino acid Gb3 mimic peptide. We have engineered the Gb3 mimic along with a cellular export signal (ompF) downstream of AHL(quorum sensing molecule) inducible promoter (pLuxR). We also plan to prevent further toxin production by inhibiting the biofilm formation of shigatoxigenic E.coli using indole-3-acetaldehyde (I3A). We expect to validate our approach through functional assays and in silico modelling. Our findings can potentially initiate a new perspective of tackling Shiga toxicosis using synthetic biology tools.
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<div class="col-md-3">
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<img src="https://static.igem.org/mediawiki/2013/6/61/Iitm13_team_pic.JPG" width="100%" style="
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    height: 100%;
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" data-src="holder.js/500x500/auto" alt="500x500">
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        </div>
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          <h2 class="featurette-heading">Oh yeah, it's that good. <span class="text-muted">See for yourself.</span></h2>
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          <p class="lead">Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod semper. Praesent commodo cursus magna, vel scelerisque nisl consectetur. Fusce dapibus, tellus ac cursus commodo.</p>
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        </div>
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      </div>
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      <hr class="featurette-divider">
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      <div class="row featurette">
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        <div class="col-md-7">
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          <h2 class="featurette-heading">And lastly, this one. <span class="text-muted">Checkmate.</span></h2>
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          <p class="lead">Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod semper. Praesent commodo cursus magna, vel scelerisque nisl consectetur. Fusce dapibus, tellus ac cursus commodo.</p>
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Latest revision as of 02:47, 28 September 2013

Indian Institute of Technology Madras

Shiga toxin, a worldwide menace, has killed over 1 million people to date and continues to afflict almost 150 million people each year. Currently, there is no treatment for Shiga toxicosis and it leads to complications in the human system like hemolytic uremic syndrome (HUS) and renal failure. Here, we propose a two-fold, novel synthetic biology approach to combat the lethal effect of the toxin. We aim to neutralize the already produced toxin through a nine amino acid Gb3 mimic peptide. We have engineered the Gb3 mimic along with a cellular export signal (ompF) downstream of AHL(quorum sensing molecule) inducible promoter (pLuxR). We also plan to prevent further toxin production by inhibiting the biofilm formation of shigatoxigenic E.coli using indole-3-acetaldehyde (I3A). We expect to validate our approach through functional assays and in silico modelling. Our findings can potentially initiate a new perspective of tackling Shiga toxicosis using synthetic biology tools.