Team:Hong Kong HKUST/Wetlab

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Latest revision as of 23:03, 27 September 2013

Wetlab

The wet lab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely: (1) cell viability and fatty acid quantification, (2) fatty acid sensing mechanism, (3) mitochondrial leader sequence, and (4) construction of glyoxylate shunt. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively and useful data were collected. These can be retrieved from Notebook and Protocols pages for details of experimental flows. The BioBrick, researcher, environmental and university safety issues can be found in Safety page. In terms of project process, some experiments could not be completed due to time constrains and they are outlined in Future Work page.

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 <br><br><br><div id="slide"><center><h3 class="title">Wetlab</h3></center><p id="yo"</div>
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-The wetlab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely: fatty acid sensing and cell transfection, promoter construction and characterization, mitochondrial leader sequence (MLS) construction, and the implementation of glyoxylate shunt genes <i>aceA</i> and <i>aceB</i>. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively and useful data was collected. These can be retrieved from the 'Data' page. However, the time allowance is not enough for us to finish all of what we wanted to do. The remainder is listed under 'Future Work'. In addition, protocols used in the lab and issues concerning lab safety are included under this section.
+The wet lab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely: (1) cell viability and fatty acid quantification, (2) fatty acid sensing mechanism, (3) mitochondrial leader sequence, and (4) construction of glyoxylate shunt. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively and useful data were collected. These can be retrieved from <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/notebook">Notebook</a> and <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/protocols">Protocols</a> pages for details of experimental flows. The BioBrick, researcher, environmental and university safety issues can be found in <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/safety">Safety</a> page. In terms of project process, some experiments could not be completed due to time constrains and they are outlined in <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/future">Future Work</a> page.
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 <div id="interview"><h3>Notebook</h3><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/notebook"><img src="https://static.igem.org/mediawiki/2013/c/cc/Note.png" style="padding-left:0px;margin-top:-8px;width:80%;"></a></center></div>
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 <div id="article"><h3>Safety</h3><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/safety"><img src="https://static.igem.org/mediawiki/2013/7/73/Safe.png" style="padding-left:0px;padding-top:-3px;width:75%;"></a></center></div></div>
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