Team:BIOSINT Mexico/Results
From 2013.igem.org
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- | [[File:Piezasgens.jpg| | + | [[File:Piezasgens.jpg|right|300px]] |
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+ | Plasmid extraction from synthetized parts | ||
+ | pUC57: 2710 bp | ||
*In the lane 1 is the ladder of 5000 bp | *In the lane 1 is the ladder of 5000 bp | ||
- | *The sample of the lane 3 is the promoter 1 | + | *The sample of the lane 3 is the promoter 1, inserted sequence: 103 bp |
- | *The sample of the lane 5 is the promoter 2 | + | *The sample of the lane 5 is the promoter 2, inserted sequence: 103 bp |
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+ | *The sample of the lane 7 is the RBS, inserted sequence: 76 bp | ||
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+ | ---- | ||
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+ | [[File:Piezas_PsB1C3.jpg|right|300px]] | ||
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+ | Parts clonning in pSB1C3: 2070 bp | ||
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+ | *In the lane 1 is the ladder of 3000 bp | ||
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+ | *The sample of the lane 3 is the promoter 2 | ||
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+ | *The sample of the lane 4 is the promoter 1 | ||
*The sample of the lane 7 is the RBS | *The sample of the lane 7 is the RBS | ||
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+ | ---- | ||
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+ | [[File:Plasmid_GFP.jpg|right|300px]] | ||
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+ | Ligation Promoters with GFP | ||
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+ | *In the lane 1 is the ladder of 5000 bp | ||
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+ | *The sample of the lane 3 is the Plasmid GFP | ||
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+ | *The sample of the lane 5 is the Plasmid GFP | ||
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+ | ---- | ||
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+ | [[File:P1_expression.jpg|center|300px]] | ||
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+ | GFP expression for L.Plantarum promoter cloned in E.coli | ||
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+ | ---- | ||
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+ | [[File:P2_expression.jpg|center|300px]] | ||
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+ | GFP expression for L.Plantarum promoter 2 cloned in E.coli |
Latest revision as of 02:57, 28 September 2013
Results
Plasmid extraction from synthetized parts
pUC57: 2710 bp
- In the lane 1 is the ladder of 5000 bp
- The sample of the lane 3 is the promoter 1, inserted sequence: 103 bp
- The sample of the lane 5 is the promoter 2, inserted sequence: 103 bp
- The sample of the lane 7 is the RBS, inserted sequence: 76 bp
Parts clonning in pSB1C3: 2070 bp
- In the lane 1 is the ladder of 3000 bp
- The sample of the lane 3 is the promoter 2
- The sample of the lane 4 is the promoter 1
- The sample of the lane 7 is the RBS
Ligation Promoters with GFP
- In the lane 1 is the ladder of 5000 bp
- The sample of the lane 3 is the Plasmid GFP
- The sample of the lane 5 is the Plasmid GFP
GFP expression for L.Plantarum promoter cloned in E.coli
GFP expression for L.Plantarum promoter 2 cloned in E.coli