"

From 2013.igem.org

Revision as of 22:42, 27 September 2013 (view source) Mague (Talk | contribs) ← Older edit		Latest revision as of 02:57, 28 September 2013 (view source) Gabyqz (Talk | contribs)
(7 intermediate revisions not shown)
Line 9:		Line 9:
	[[File:Piezasgens.jpg|right|300px]]		[[File:Piezasgens.jpg|right|300px]]
		+	Plasmid extraction from synthetized parts
-		+	pUC57: 2710 bp
	*In the lane 1 is the ladder of 5000 bp		*In the lane 1 is the ladder of 5000 bp
-	*The sample of the lane 3 is the promoter 1	+	*The sample of the lane 3 is the promoter 1, inserted sequence: 103 bp
-	*The sample of the lane 5 is the promoter 2	+	*The sample of the lane 5 is the promoter 2, inserted sequence: 103 bp
-	*The sample of the lane 7 is the RBS	+	*The sample of the lane 7 is the RBS, inserted sequence: 76 bp
Line 35:		Line 36:
		+
		+
		+
		+
		+	----
Line 44:		Line 50:
-	Pieces clonning in pSB1C3	+	Parts clonning in pSB1C3: 2070 bp
-	In the lane 1 is the ladder of 3000 bp	+	*In the lane 1 is the ladder of 3000 bp
-	The sample of the lane 3 is the promoter 2	+	*The sample of the lane 3 is the promoter 2
-	The sample of the lane 4 is the promoter 1	+	*The sample of the lane 4 is the promoter 1
-	The sample of the lane 7 is the RBS	+	*The sample of the lane 7 is the RBS
Line 71:		Line 77:
		+	----
Line 79:		Line 86:
-	Plasmid with GFP	+	Ligation Promoters with GFP
-	In the lane 1 is the ladder of 5000 bp	+	*In the lane 1 is the ladder of 5000 bp
-	The sample of the lane 3 is the Plasmid GFP	+	*The sample of the lane 3 is the Plasmid GFP
-	The sample of the lane 5 is the Plasmid GFP	+	*The sample of the lane 5 is the Plasmid GFP
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+
		+	----
		+
		+	[[File:P1_expression.jpg|center|300px]]
		+
		+	GFP expression for L.Plantarum promoter cloned in E.coli
		+
		+	----
		+
		+	[[File:P2_expression.jpg|center|300px]]
		+
		+	GFP expression for L.Plantarum promoter 2 cloned in E.coli

---

Latest revision as of 02:57, 28 September 2013

---

Results

---

---

Plasmid extraction from synthetized parts

pUC57: 2710 bp

• In the lane 1 is the ladder of 5000 bp

• The sample of the lane 3 is the promoter 1, inserted sequence: 103 bp

• The sample of the lane 5 is the promoter 2, inserted sequence: 103 bp

• The sample of the lane 7 is the RBS, inserted sequence: 76 bp

---

Parts clonning in pSB1C3: 2070 bp

• In the lane 1 is the ladder of 3000 bp

• The sample of the lane 3 is the promoter 2

• The sample of the lane 4 is the promoter 1

• The sample of the lane 7 is the RBS

---

Ligation Promoters with GFP

• In the lane 1 is the ladder of 5000 bp

• The sample of the lane 3 is the Plasmid GFP

• The sample of the lane 5 is the Plasmid GFP

---

GFP expression for L.Plantarum promoter cloned in E.coli

---

GFP expression for L.Plantarum promoter 2 cloned in E.coli