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Experiment 4: Cell Viability & Fatty Acid Quantification
Measuring cell viability at different fatty acid concentration & measure fatty acid uptake rate
Team:Hong Kong HKUST/results
From 2013.igem.org
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<div id="presentation"><h3>Experiment 3: Fatty Acid Sensing Mechanism</h3><div id="pre-img"><br><img src="https://static.igem.org/mediawiki/2013/4/4f/Expt1.png" style="padding-left:5px;padding-top:5px;width:90%;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment/exp2"><img src="http://w1ggroup.com/devere/img/more_off.png" style="width:80%;height:40px;padding-top:10px;"></a></center></div><div id="pre-desc"><p id="pre-desc-text"><br>Introduce inducible system that allows tunable fatty acid uptake by sensing fatty acid concentration</p></div></div> | <div id="presentation"><h3>Experiment 3: Fatty Acid Sensing Mechanism</h3><div id="pre-img"><br><img src="https://static.igem.org/mediawiki/2013/4/4f/Expt1.png" style="padding-left:5px;padding-top:5px;width:90%;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment/exp2"><img src="http://w1ggroup.com/devere/img/more_off.png" style="width:80%;height:40px;padding-top:10px;"></a></center></div><div id="pre-desc"><p id="pre-desc-text"><br>Introduce inducible system that allows tunable fatty acid uptake by sensing fatty acid concentration</p></div></div> | ||
| - | <div id="article"><h3>Experiment 4: Cell Viability & Fatty Acid Quantification</h3><div id="pre-img"><img src="https://static.igem.org/mediawiki/2013/f/fa/Expt0.png" style="padding-left:2px;padding-top:5px;width:90%;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment/exp1"><img src="http://w1ggroup.com/devere/img/more_off.png" style="width:80%;height:40px;padding-top:10px;"></a></center></div><div id="pre-desc"><p id="pre-desc-text">Measuring cell viability at different fatty acid concentration & measure fatty acid uptake rate</p></div></div> | + | <div id="article"><h3>Experiment 4: Cell Viability & Fatty Acid Quantification</h3><div id="pre-img"><br><img src="https://static.igem.org/mediawiki/2013/f/fa/Expt0.png" style="padding-left:2px;padding-top:5px;width:90%;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment/exp1"><img src="http://w1ggroup.com/devere/img/more_off.png" style="width:80%;height:40px;padding-top:10px;"></a></center></div><div id="pre-desc"><br><p id="pre-desc-text">Measuring cell viability at different fatty acid concentration & measure fatty acid uptake rate</p></div></div> |
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<p id="yo">Our project was first proposed in mid-March and after a series of improvement and elaboration, we started our wet lab work from mid-June to late-September. After 3 months of memorable diligent work, we are proud to claim that we have achieved the results below: | <p id="yo">Our project was first proposed in mid-March and after a series of improvement and elaboration, we started our wet lab work from mid-June to late-September. After 3 months of memorable diligent work, we are proud to claim that we have achieved the results below: | ||
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<li>Successfully constructed two parts</a> carrying mitochondrial leader sequence (MLS) compatible with RFC 10 and RFC 25 assembly standards respectively, (<a href="http://parts.igem.org/Part:BBa_K1119000">BBa_K1119000</a>, <a href="http://parts.igem.org/Part:BBa_K1119001">BBa_K1119001</a>).</li> | <li>Successfully constructed two parts</a> carrying mitochondrial leader sequence (MLS) compatible with RFC 10 and RFC 25 assembly standards respectively, (<a href="http://parts.igem.org/Part:BBa_K1119000">BBa_K1119000</a>, <a href="http://parts.igem.org/Part:BBa_K1119001">BBa_K1119001</a>).</li> | ||
<li>Successfully constructed </a>parts containing the <i>aceA</i> and <i>aceB</i> elements of the glyoxylate shunt (<a href="http://parts.igem.org/Part:BBa_K1119002">BBa_K1119002</a>, <a href="http://parts.igem.org/Part:BBa_K1119003">BBa_K1119003</a>). </li> | <li>Successfully constructed </a>parts containing the <i>aceA</i> and <i>aceB</i> elements of the glyoxylate shunt (<a href="http://parts.igem.org/Part:BBa_K1119002">BBa_K1119002</a>, <a href="http://parts.igem.org/Part:BBa_K1119003">BBa_K1119003</a>). </li> | ||
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<li>Successfully constructed a part containing the constitutive CMV promoter (<a href="http://parts.igem.org/Part:BBa_K1119006">BBa_K1119006</a>).</li> | <li>Successfully constructed a part containing the constitutive CMV promoter (<a href="http://parts.igem.org/Part:BBa_K1119006">BBa_K1119006</a>).</li> | ||
<li>Successfully characterized the constitutive CMV promoter in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119008">BBa_K1119008</a>).</li> | <li>Successfully characterized the constitutive CMV promoter in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119008">BBa_K1119008</a>).</li> | ||
| + | <li>Successfully constructed a part containing the constitutive regulatory promoter of Human Elongation Factor 1-alpha (<a href="http://parts.igem.org/Part:BBa_K1119010">BBa_K1119010</a>).</li> | ||
| + | <li>Successfully characterized the constitutive EF-1alpha promoter in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119011">BBa_K1119011</a>).</li> | ||
<li>Successfully characterized the mitochondrial leader sequence (MLS) in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119009">BBa_K1119009</a>).</li> | <li>Successfully characterized the mitochondrial leader sequence (MLS) in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119009">BBa_K1119009</a>).</li> | ||
<li>Successfully tested HepG2 cell viability in different sodium palmitate concentrations. </li> | <li>Successfully tested HepG2 cell viability in different sodium palmitate concentrations. </li> | ||
Latest revision as of 12:53, 28 October 2013
Result
Experiment 1: Glyoxylate Shunt
Introduce glyoxylate enzymes responsible for the shunt
Experiment 2: Protein Trafficking
Target ACE proteins into mitochondria
Experiment 3: Fatty Acid Sensing Mechanism
Introduce inducible system that allows tunable fatty acid uptake by sensing fatty acid concentration
Our project was first proposed in mid-March and after a series of improvement and elaboration, we started our wet lab work from mid-June to late-September. After 3 months of memorable diligent work, we are proud to claim that we have achieved the results below:
- Successfully constructed two parts carrying mitochondrial leader sequence (MLS) compatible with RFC 10 and RFC 25 assembly standards respectively, (BBa_K1119000, BBa_K1119001).
- Successfully constructed parts containing the aceA and aceB elements of the glyoxylate shunt (BBa_K1119002, BBa_K1119003).
- Successfully constructed a part containing the constitutive CMV promoter (BBa_K1119006).
- Successfully characterized the constitutive CMV promoter in HEK293FT cells (BBa_K1119008).
- Successfully constructed a part containing the constitutive regulatory promoter of Human Elongation Factor 1-alpha (BBa_K1119010).
- Successfully characterized the constitutive EF-1alpha promoter in HEK293FT cells (BBa_K1119011).
- Successfully characterized the mitochondrial leader sequence (MLS) in HEK293FT cells (BBa_K1119009).
- Successfully tested HepG2 cell viability in different sodium palmitate concentrations.
