Team:BIOSINT Mexico/Results

From 2013.igem.org

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[[File:Piezasgens.jpg|right|300px]]
[[File:Piezasgens.jpg|right|300px]]
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Plasmid extraction from synthetized parts
pUC57: 2710 bp
pUC57: 2710 bp
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Pieces clonning in pSB1C3: 2070 bp
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Parts clonning in pSB1C3: 2070 bp
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In the lane 1 is the ladder of 3000 bp
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*In the lane 1 is the ladder of 3000 bp
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The sample of the lane 3 is the promoter 2
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*The sample of the lane 3 is the promoter 2
   
   
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The sample of the lane 4 is the promoter 1
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*The sample of the lane 4 is the promoter 1
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The sample of the lane 7 is the RBS
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*The sample of the lane 7 is the RBS
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Plasmid with GFP
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Ligation Promoters with GFP
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In the lane 1 is the ladder of 5000 bp
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*In the lane 1 is the ladder of 5000 bp
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The sample of the lane 3 is the Plasmid GFP
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*The sample of the lane 3 is the Plasmid GFP
   
   
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The sample of the lane 5 is the Plasmid GFP
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*The sample of the lane 5 is the Plasmid GFP
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[[File:P1_expression.jpg|center|300px]]
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GFP expression for L.Plantarum promoter cloned in E.coli
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[[File:P2_expression.jpg|center|300px]]
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GFP expression for L.Plantarum promoter 2 cloned in E.coli

Latest revision as of 02:57, 28 September 2013

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Results



Piezasgens.jpg

Plasmid extraction from synthetized parts

pUC57: 2710 bp

  • In the lane 1 is the ladder of 5000 bp
  • The sample of the lane 3 is the promoter 1, inserted sequence: 103 bp
  • The sample of the lane 5 is the promoter 2, inserted sequence: 103 bp
  • The sample of the lane 7 is the RBS, inserted sequence: 76 bp













Piezas PsB1C3.jpg



Parts clonning in pSB1C3: 2070 bp


  • In the lane 1 is the ladder of 3000 bp
  • The sample of the lane 3 is the promoter 2
  • The sample of the lane 4 is the promoter 1
  • The sample of the lane 7 is the RBS











Plasmid GFP.jpg



Ligation Promoters with GFP


  • In the lane 1 is the ladder of 5000 bp
  • The sample of the lane 3 is the Plasmid GFP
  • The sample of the lane 5 is the Plasmid GFP












P1 expression.jpg

GFP expression for L.Plantarum promoter cloned in E.coli


P2 expression.jpg

GFP expression for L.Plantarum promoter 2 cloned in E.coli