Team:BIOSINT Mexico/Results
From 2013.igem.org
(Difference between revisions)
(4 intermediate revisions not shown) | |||
Line 9: | Line 9: | ||
[[File:Piezasgens.jpg|right|300px]] | [[File:Piezasgens.jpg|right|300px]] | ||
- | + | Plasmid extraction from synthetized parts | |
pUC57: 2710 bp | pUC57: 2710 bp | ||
Line 50: | Line 50: | ||
- | + | Parts clonning in pSB1C3: 2070 bp | |
- | In the lane 1 is the ladder of 3000 bp | + | *In the lane 1 is the ladder of 3000 bp |
- | The sample of the lane 3 is the promoter 2 | + | *The sample of the lane 3 is the promoter 2 |
- | The sample of the lane 4 is the promoter 1 | + | *The sample of the lane 4 is the promoter 1 |
- | + | ||
- | + | ||
+ | *The sample of the lane 7 is the RBS | ||
Line 87: | Line 86: | ||
- | + | Ligation Promoters with GFP | |
- | In the lane 1 is the ladder of 5000 bp | + | *In the lane 1 is the ladder of 5000 bp |
- | The sample of the lane 3 is the Plasmid GFP | + | *The sample of the lane 3 is the Plasmid GFP |
- | The sample of the lane 5 is the Plasmid GFP | + | *The sample of the lane 5 is the Plasmid GFP |
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | ---- | ||
+ | |||
+ | [[File:P1_expression.jpg|center|300px]] | ||
+ | |||
+ | GFP expression for L.Plantarum promoter cloned in E.coli | ||
+ | |||
+ | ---- | ||
+ | |||
+ | [[File:P2_expression.jpg|center|300px]] | ||
+ | |||
+ | GFP expression for L.Plantarum promoter 2 cloned in E.coli |
Latest revision as of 02:57, 28 September 2013
Results
Plasmid extraction from synthetized parts
pUC57: 2710 bp
- In the lane 1 is the ladder of 5000 bp
- The sample of the lane 3 is the promoter 1, inserted sequence: 103 bp
- The sample of the lane 5 is the promoter 2, inserted sequence: 103 bp
- The sample of the lane 7 is the RBS, inserted sequence: 76 bp
Parts clonning in pSB1C3: 2070 bp
- In the lane 1 is the ladder of 3000 bp
- The sample of the lane 3 is the promoter 2
- The sample of the lane 4 is the promoter 1
- The sample of the lane 7 is the RBS
Ligation Promoters with GFP
- In the lane 1 is the ladder of 5000 bp
- The sample of the lane 3 is the Plasmid GFP
- The sample of the lane 5 is the Plasmid GFP
GFP expression for L.Plantarum promoter cloned in E.coli
GFP expression for L.Plantarum promoter 2 cloned in E.coli