"
Team:BIOSINT Mexico/Construction
From 2013.igem.org
(Difference between revisions)
(→Kill switch) |
|||
| (12 intermediate revisions not shown) | |||
| Line 4: | Line 4: | ||
---- | ---- | ||
<html><h1 class="myowntitle">Construction</h1> </html> | <html><h1 class="myowntitle">Construction</h1> </html> | ||
| - | |||
| - | |||
| - | === | + | ===Repressor switch=== |
---- | ---- | ||
| + | [[File:Repressorbio.jpg|center|300px]] | ||
| - | |||
| - | |||
| + | :Based on the system of iGEM team Slovenia 2012, we adapted the repressor system using TAL A/KRAB. The objective is to reproduce a simple circuit in a Lactobacillus chassis. | ||
| - | + | :The main goal of our xenobiotics biosensor is to detect organochlorides in different concentrations in order to activate a correct answer. | |
| - | + | ||
| - | + | ||
| - | + | :High concentration: When the concentration is high Gal4 DBD could add to UAS and activated linA, a protein wich degrades pesticides, and at the same time express a repressor protein which inhibits the activity of the correpressor that controled the kill switch system. | |
| - | + | ||
| + | :Low concentration: When the concentration decrease, there is not enought UAS activity in the linA system so the repressor activity stops and the peptides that kill the bacteria starts to be produced. | ||
===Circuit=== | ===Circuit=== | ||
---- | ---- | ||
[[File:Diapositiva1.JPG|center|700px]] | [[File:Diapositiva1.JPG|center|700px]] | ||
Latest revision as of 04:20, 28 September 2013
SmartPro
Smartpro
Construction
Repressor switch
- Based on the system of iGEM team Slovenia 2012, we adapted the repressor system using TAL A/KRAB. The objective is to reproduce a simple circuit in a Lactobacillus chassis.
- The main goal of our xenobiotics biosensor is to detect organochlorides in different concentrations in order to activate a correct answer.
- High concentration: When the concentration is high Gal4 DBD could add to UAS and activated linA, a protein wich degrades pesticides, and at the same time express a repressor protein which inhibits the activity of the correpressor that controled the kill switch system.
- Low concentration: When the concentration decrease, there is not enought UAS activity in the linA system so the repressor activity stops and the peptides that kill the bacteria starts to be produced.
Circuit
