Team:BIOSINT Mexico/Results

From 2013.igem.org

(Difference between revisions)
 
Line 60: Line 60:
*The sample of the lane 7 is the RBS
*The sample of the lane 7 is the RBS
-
 
Line 95: Line 94:
   
   
*The sample of the lane 5 is the Plasmid GFP
*The sample of the lane 5 is the Plasmid GFP
 +
Line 117: Line 117:
[[File:P1_expression.jpg|center|300px]]
[[File:P1_expression.jpg|center|300px]]
-
 
GFP expression for L.Plantarum promoter cloned in E.coli
GFP expression for L.Plantarum promoter cloned in E.coli
 +
 +
----
 +
 +
[[File:P2_expression.jpg|center|300px]]
 +
 +
GFP expression for L.Plantarum promoter 2 cloned in E.coli

Latest revision as of 02:57, 28 September 2013

SmartPro

Smartpro

Igem LogotipoDefinitivo



Results



Piezasgens.jpg

Plasmid extraction from synthetized parts

pUC57: 2710 bp

  • In the lane 1 is the ladder of 5000 bp
  • The sample of the lane 3 is the promoter 1, inserted sequence: 103 bp
  • The sample of the lane 5 is the promoter 2, inserted sequence: 103 bp
  • The sample of the lane 7 is the RBS, inserted sequence: 76 bp













Piezas PsB1C3.jpg



Parts clonning in pSB1C3: 2070 bp


  • In the lane 1 is the ladder of 3000 bp
  • The sample of the lane 3 is the promoter 2
  • The sample of the lane 4 is the promoter 1
  • The sample of the lane 7 is the RBS











Plasmid GFP.jpg



Ligation Promoters with GFP


  • In the lane 1 is the ladder of 5000 bp
  • The sample of the lane 3 is the Plasmid GFP
  • The sample of the lane 5 is the Plasmid GFP












P1 expression.jpg

GFP expression for L.Plantarum promoter cloned in E.coli


P2 expression.jpg

GFP expression for L.Plantarum promoter 2 cloned in E.coli