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Team:DTU-Denmark/Notebook/6 July 2013
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| + | {{:Team:DTU-Denmark/Templates/StartPage|6 July 2013}} | ||
| + | Navigate to the [[Team:DTU-Denmark/Notebook/5_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/7_July_2013|Next]] Entry | ||
=208 lab= | =208 lab= | ||
| - | + | <hr/> | |
== Main purposes today == | == Main purposes today == | ||
| - | + | <hr/> | |
| - | Extract periplasmic contents from | + | Extract periplasmic contents from TAT21, SEC2, TAT31a, TAT22 transformants. |
| - | + | ||
==Who was in the lab== | ==Who was in the lab== | ||
| - | + | <hr/> | |
Kristian, Helen | Kristian, Helen | ||
==Procedure== | ==Procedure== | ||
| + | <hr/> | ||
| + | Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html link]). Used smaller amount though. | ||
| - | + | Then, viewed the periplasmic and cytoplasmic cell contents under UV light. | |
| - | + | ||
| - | Then, viewed the periplasmic and cytoplasmic cell contents under UV light. | + | |
== Conclusion from today == | == Conclusion from today == | ||
| + | <hr/> | ||
| + | The Hello World project was a success -- the periplasm contains GFP and the cytoplasm contains RFP. | ||
| - | + | [[File:DTU-Denmark_HelloWorld_Sucrose_A+D+C_2.jpg|600px]] | |
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| + | Navigate to the [[Team:DTU-Denmark/Notebook/5_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/7_July_2013|Next]] Entry | ||
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| + | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Latest revision as of 20:39, 16 September 2013
6 July 2013
Contents |
208 lab
Main purposes today
Extract periplasmic contents from TAT21, SEC2, TAT31a, TAT22 transformants.
Who was in the lab
Kristian, Helen
Procedure
Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html link]). Used smaller amount though.
Then, viewed the periplasmic and cytoplasmic cell contents under UV light.
Conclusion from today
The Hello World project was a success -- the periplasm contains GFP and the cytoplasm contains RFP.
Navigate to the Previous or the Next Entry
