Latest revision as of 20:39, 16 September 2013

6 July 2013

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Extract periplasmic contents from TAT21, SEC2, TAT31a, TAT22 transformants.

Kristian, Helen

Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html link]). Used smaller amount though.

Then, viewed the periplasmic and cytoplasmic cell contents under UV light.

The Hello World project was a success -- the periplasm contains GFP and the cytoplasm contains RFP.

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+{{:Team:DTU-Denmark/Templates/StartPage|6 July 2013}}
+Navigate to the [[Team:DTU-Denmark/Notebook/5_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/7_July_2013|Next]] Entry
 =208 lab=
+<hr/>
 == Main purposes today ==
+<hr/>
 Extract periplasmic contents from TAT21, SEC2, TAT31a, TAT22 transformants.
 ==Who was in the lab==
+<hr/>
 Kristian, Helen
 ==Procedure==
+<hr/>
+Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html  link]). Used smaller amount though.
-Followed Openwetware procedure for periplasmic extraction.
+Then, viewed the periplasmic and cytoplasmic cell contents under UV light.
-Then, viewed the periplasmic and cytoplasmic cell contents under UV light.
 == Conclusion from today ==
+<hr/>
+The Hello World project was a success -- the periplasm contains GFP and the cytoplasm contains RFP.
-The Hello World project was a success -- the periplasm contains GFP and the cytoplasm contains RFP.
+[[File:DTU-Denmark_HelloWorld_Sucrose_A+D+C_2.jpg|600px]]
+Navigate to the [[Team:DTU-Denmark/Notebook/5_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/7_July_2013|Next]] Entry
-[[File:Example.jpg]]
+{{:Team:DTU-Denmark/Templates/EndPage}}