Team:DTU-Denmark/Notebook/6 July 2013

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{{:Team:DTU-Denmark/Templates/StartPage|6 July 2013}}
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=208 lab=
=208 lab=
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<hr/>
== Main purposes today ==
== Main purposes today ==
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Extract periplasmic contents from TAT21, SEC2, TAT31a, TAT22 transformants.
Extract periplasmic contents from TAT21, SEC2, TAT31a, TAT22 transformants.
==Who was in the lab==
==Who was in the lab==
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Kristian, Helen
Kristian, Helen
==Procedure==
==Procedure==
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Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html  link]). Used smaller amount though.
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Followed Openwetware procedure for periplasmic extraction.
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Then, viewed the periplasmic and cytoplasmic cell contents under UV light.
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Then, viewed the periplasmic and cytoplasmic cell contents under UV light.  
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== Conclusion from today ==
== Conclusion from today ==
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The Hello World project was a success -- the periplasm contains GFP and the cytoplasm contains RFP.
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The Hello World project was a success -- the periplasm contains GFP and the cytoplasm contains RFP.
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[[File:DTU-Denmark_HelloWorld_Sucrose_A+D+C_2.jpg|600px]]
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Navigate to the [[Team:DTU-Denmark/Notebook/5_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/7_July_2013|Next]] Entry
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[[File:DTU-Denmark_HelloWorld_Sucrose_A+D+C_2.jpg]]
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{{:Team:DTU-Denmark/Templates/EndPage}}

Latest revision as of 20:39, 16 September 2013

6 July 2013

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Contents

208 lab


Main purposes today


Extract periplasmic contents from TAT21, SEC2, TAT31a, TAT22 transformants.

Who was in the lab


Kristian, Helen

Procedure


Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html link]). Used smaller amount though.

Then, viewed the periplasmic and cytoplasmic cell contents under UV light.

Conclusion from today


The Hello World project was a success -- the periplasm contains GFP and the cytoplasm contains RFP.

DTU-Denmark HelloWorld Sucrose A+D+C 2.jpg


Navigate to the Previous or the Next Entry