Team:Paris Bettencourt/Backbones

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      <b> We upgraded the standard biobrick shipping vector pSB1C3 with a new set of 4 ori’s and antibiotic resistance cassettes so that they can easily be co-expressed.  The system of 4 backbones all have compatible origins and resistance cassettes that allows for </b>
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      <p><b> up to 4 back bones to be expressed in a single host bacteria. Additionally, we retained compatibility with Duet expression vectors which we commonly used in our project. </b></p>
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  <h2><a href="http://parts.igem.org/Part:BBa_K1137003">pSB9S1</a></h2>
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      Contains the mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3.  The origin of replication has been replaced with CloDF13 replicon with a copy number of 20 – 40 per cell.  The cat gene has been replaced with aadA conveying spectinomycin and streptomycin resistance.
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<h2><a href="http://parts.igem.org/Part:BBa_K1137005">pSB10K1</a></h2>
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      <p>contains mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3.  The origin of replication has been replaced with ColA replicon with a copy number of 20 – 40 per cell.  The cat gene has been replaced with kan cassette conveying kanamycin resistance.
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<h2><a href="http://parts.igem.org/Part:BBa_K1137004">pSB3C7</a></h2>
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      <p>contains mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3.  The origin of replication has been replaced with P15A replicon with a copy number of 10-12 per cell.  The cat gene has been maintained conveying chloramphenicol resistance.
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<h2><a href="http://parts.igem.org/Part:BBa_K1137006">pSB6A3</a></h2>
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      <p>contains mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3.  The origin of replication has been replaced with ColE1 replicon derived from pBR322 with a copy number of approximately 40 per cell.  The cat gene has been replaced with ampR cassette conveying ampicillin resistance.
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Latest revision as of 22:56, 4 October 2013

We upgraded the standard biobrick shipping vector pSB1C3 with a new set of 4 ori’s and antibiotic resistance cassettes so that they can easily be co-expressed. The system of 4 backbones all have compatible origins and resistance cassettes that allows for

up to 4 back bones to be expressed in a single host bacteria. Additionally, we retained compatibility with Duet expression vectors which we commonly used in our project.

pSB9S1

Contains the mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with CloDF13 replicon with a copy number of 20 – 40 per cell. The cat gene has been replaced with aadA conveying spectinomycin and streptomycin resistance.

pSB10K1

contains mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with ColA replicon with a copy number of 20 – 40 per cell. The cat gene has been replaced with kan cassette conveying kanamycin resistance.

pSB3C7

contains mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with P15A replicon with a copy number of 10-12 per cell. The cat gene has been maintained conveying chloramphenicol resistance.

pSB6A3

contains mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with ColE1 replicon derived from pBR322 with a copy number of approximately 40 per cell. The cat gene has been replaced with ampR cassette conveying ampicillin resistance.

Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
team2013@igem-paris.org
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